Subjects: Biology >> Botany >> Applied botany submitted time 2023-10-22 Cooperative journals: 《广西植物》
Abstract：为研究檀香NDH 脱氢酶基因的功能和调控机制，以檀香心材为材料，利用RACE 技术克隆SaNDH6 基因的全长序列，利用实时荧光定量PCR（RT-qPCR）技术分析其组织 和激素处理后的表达模式，在拟南芥原生质体观测其亚细胞定位，利用PlantCARE 分析 SaNDH6 起始密码子ATG 上游2 kb 的启动子序列，同时运用PlantRegMap 预测可能与其结 合的转录因子。结果表明：（1）SaNDH6 编码303 个氨基酸，为疏水蛋白，亚细胞定位于 叶绿体。（2）进化树分析表明，檀香SaNDH6 与木本植物NDH6 进化关系较近。（3）PlantCARE 分析发现，SaNDH6 启动子中除含有ACE、AE-box、Box 4、G-Box 和GT1-motif 等大量光 响应元件外，同时还有茉莉酸甲酯（MeJA）反应元件CGTCA-motif 和TGACG-motif，赤霉 素（GA）响应元件P-box，以及防御和胁迫响应元件TC-rich repeats 等。（4）通过PlantRegMap 分析发现，有76 个转录因子可能与SaNDH6 启动子结合，其中ERF 家族最多，达到了40 个。（5）SaNDH6 在檀香的根、心材、叶片和愈伤组织中均有表达，其中在叶片中的表达 量较高；用110-4 molL-1 的MeJA 和GA3 分别处理檀香愈伤组织后，SaNDH6 的表达均显 著升高。综上结果表明，檀香SaNDH6 为核基因编码的蛋白，受光和激素等诱导表达，可 能参与檀香逆境胁迫反应的过程。
Subjects: Biology >> Botany >> Applied botany submitted time 2021-12-19 Cooperative journals: 《广西植物》
Abstract： The purpose of this study was to clone the RT genes of Ty1-copia-like retrotransposons, and to provide sequences basis for isolating the full-length sequences of Ty1-copia-like retrotransposons and studying their function in genus Arachis. Degenerate primers were designed according to the conserved region of RT genes, the genomic DNA of wild peanut species “Arachis duranensis” with AA genome was amplified by PCR using the degenerated primers. The amplified targeted bands were recovered, cloned and sequenced, and then sequences were analyzed through bioinformatics strategy. The results were as follows: (1) The amplified targeted bands were all about 260 bp in size. Forty-one and twenty-seven RT genes sequences were cloned from the two wild peanut species respectively. The length of sixty-eight sequences varied from 256 bp to 270 bp. The proportion of AT, AT/GC and the similarity between nucleotide sequences ranged from 55.86% to 68.42%, 1.27% to 2.17%, and 49.8% to 99.2% respectively, showing a higher heterogeneity. (2) The sixty-eight sequences were divided into six families, family I and family IV were the main components. (3) Nineteen of sixty-eight sequences had nonsense mutations, and Arachis duranensis (PI219823) had a higher nonsense mutations rate than Arachis duranensis (PI262133). (4) The similarity between amino acid sequences ranged from 4.7% to 100%, showing high heterogeneity. (5) The tertiary structures of proteins representing sequences in each family were basically similar in overall configuration, but there were great differences in the number of helix structures, folding structures, turns and hydrogen bonds. (6) The conserved motifs among sequences were generally consistent, but there were also some variations, showing a certain degree of heterogeneity. The phylogenetic tree divided sixty-eight sequences into ten classes. Most of sequences are clustered in A and B classes. (7) Some of RT genes sequences from two wild peanut species with AA genome were closely related to RT genes sequences from other plant species, which indicated that there might be transposon horizontal transmission between them. This study laid a foundation for the development and application of new molecular markers based on Ty1-copia-like retrotransposons in genus Arachis.
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