Subjects: Biology >> Botany >> Applied botany submitted time 2018-12-04 Cooperative journals: 《广西植物》
Abstract：青钱柳是中国特有的胡桃科青钱柳属单种属速生乔木，有重要地医用价值，被誉为医学界的第三棵树，但其种苗繁育难大、效率低，因此，以青钱柳幼嫩茎段为外植体，研究其种苗快速繁殖技术，为其优良无性系的规模化繁育奠定基础。研究结果表明，青钱柳最佳采样时间为 4—6 月，最佳外植体为轻微木质化茎段；最佳的外植体表面消毒方法为用 0.1% HgCl2浸泡 5～7 min，消毒成功率 54.1%，无菌外植体存活率 88.7%；初代芽诱导培养基为MS+6-BA 2.0 mg∙L-1+IBA 0.2 mg∙L-1+蔗糖 30.0 g∙L-1，芽诱导率80.5%，培养 21 d 初代芽苗平均高度 3.0 cm；最佳继代增殖培养基为 MS+6-BA 0.5 mg∙L-1+IBA 0.05mg∙L-1+TIBA 0.02 mg∙L-1+蔗糖 30.0 g∙L-1，培养 35 d，增殖系数 7.0 / 35 d，平均苗高 4.5 cm，芽苗健壮且无玻璃化；生根前的壮苗培养基为 MS+6-BA 0.5 mg∙L-1+IBA 0.05 mg∙L-1+蔗糖 30.0 g∙L-1，培养 35 d，长出的芽苗高大健壮，平均苗高 6.0 cm；生根培养基为 1/2WPM+IBA 1.5 mg∙L-1+5-NGS 4.5 mg∙L-1+蔗糖 20.0g∙L-1，培养 40 d，最高生根率 83.3%；生根苗较适合的移栽基质为泥碳土，较好的移栽季节为 3—5 月和10—11 月，移栽后在遮光度 70%的大棚中培养 40 天，移栽成活率 54.3%~65.6%。
Subjects: Biology >> Botany >> Applied botany submitted time 2018-09-07 Cooperative journals: 《广西植物》
Abstract： TissuecultureandrapidpropagationtechniqueofCalotropisgiganteaL.wasstudied byusingstemsegmentsasexplantsinthispaper.Theresultsshowedthattheoptimalsterilization 32.3%. The suitable primary induction medium was MS+ sucrose 30g稬-1+ ager 3.5 g稬-1. And after 20 d culture, 3-4 cm high regenerative buds were formed. Pre-multiplication culture test showed that MS+ 6-BA1.5 mg稬-1+ NAA0.05 mg稬-1+AgNO31.0 g稬-1+ sucrose 30 g稬-1+ ager 3.5 g稬-1 was a suitable multiplication medium, proliferation coefficient was 4.6/25 d. However, during the subsequent cultivation process, using this method the seedlings were easy to vitrify. With the change of generation, the degree of vitrification has been increasing. So inhibition of vitrification was the key to success.Based on the above multiplication culture test, withAgNO3 as a vitrification inhibitor, the suitable multiplication culture medium of MS+ 6-BA 1.5 mg稬-1+ NAA 0.05 mg稬-1+ AgNO3 1.0 g稬-1+ sucrose 30 g稬-1+ ager 3.5 g稬-1 was verified. With this method, 25 d later, seedling height was 5-8 cm, proliferation coefficient was higher than 5.8/25 d and vitrification rate of multiple shoots was lower than 10%.The optimal medium for rooting was 1/2MS+ NAA1.0 mg稬-1+ sucrose 20 g稬-1 + ager 3.6 g稬-1, 14 d later, the rooting rate was 98%. The rooting seedlings were transplanted in the 70% shading greenhouse.And 30 d later,they were about 20 cm high, with 85% survival rate. This method can be used for the large-scale production ofexcellentclonalseedlingsofCalotropisgiganteaL.
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