Subjects: Biology >> Botany >> Applied botany submitted time 2022-03-18 Cooperative journals: 《广西植物》
Abstract： In order to explore the relationship between the growth of Paris polyphylla var. chinensis and the microbial community structure changes of the rhizosphere soil, the Illumina high-throughput sequencing technique was used to sequence the 16S rRNA gene of bacteria and 18S sequences of fungal at four different growth years, including 3 years, 5 years, 7 years and 9 years. The results were as follows: (1) Proteobacteria, Acidobacteria, Actinobacteria and Chloroflexi were dominant bacteria in the rhizosphere soil of P. polyphylla in different growth years. The dominant fungi phylas were Ascomycota, Basidiomycota and Mucoromycota. (2) The bacterial species in the rhizosphere of Paris polyphylla in different growth years were more abundant than fungi, and the degree of diversification was higher. The lowest point of bacterial diversity, which decreased, increased, and then decreased again with the increase of growth years showed a pattern of “И”, was at the 5th year and the highest point was at the 7th year. The fungal diversity showed a pattern of “ Λ ” that first increased and then decreased with the increase of growth years, and the highest point was at the 7th year. The abundance of bacterial communities also showed a pattern of “Λ” that first increased and then decreased with the increase of growth years, and the highest point was also at the 7th year. The abundance of fungi did not change much as the year growing. (3) The UPGMA cluster analysis showed that the microbial community structure of the rhizosphere soil had evolved significantly. The period when the bacterial community evolved more drastically was in the 7th year after planting, and the fungal community was in the 5th. (4) Spearman correlation analysis found that total nitrogen and available potassium were the main factors affecting the composition of rhizosphere soil bacteria, and Total potassium was the main factor affecting the composition of fungi. All the above results indicate that the composition and structure of the soil microbial community in the rhizosphere of wild P. polyphylla at different growth and development stages are different, and the 5-7th year is a critical period for significant changes in the diverdity of the soil microbial community.
Subjects: Biology >> Botany >> Applied botany submitted time 2018-12-04 Cooperative journals: 《广西植物》
Abstract：青钱柳是中国特有的胡桃科青钱柳属单种属速生乔木，有重要地医用价值，被誉为医学界的第三棵树，但其种苗繁育难大、效率低，因此，以青钱柳幼嫩茎段为外植体，研究其种苗快速繁殖技术，为其优良无性系的规模化繁育奠定基础。研究结果表明，青钱柳最佳采样时间为 4—6 月，最佳外植体为轻微木质化茎段；最佳的外植体表面消毒方法为用 0.1% HgCl2浸泡 5～7 min，消毒成功率 54.1%，无菌外植体存活率 88.7%；初代芽诱导培养基为MS+6-BA 2.0 mg∙L-1+IBA 0.2 mg∙L-1+蔗糖 30.0 g∙L-1，芽诱导率80.5%，培养 21 d 初代芽苗平均高度 3.0 cm；最佳继代增殖培养基为 MS+6-BA 0.5 mg∙L-1+IBA 0.05mg∙L-1+TIBA 0.02 mg∙L-1+蔗糖 30.0 g∙L-1，培养 35 d，增殖系数 7.0 / 35 d，平均苗高 4.5 cm，芽苗健壮且无玻璃化；生根前的壮苗培养基为 MS+6-BA 0.5 mg∙L-1+IBA 0.05 mg∙L-1+蔗糖 30.0 g∙L-1，培养 35 d，长出的芽苗高大健壮，平均苗高 6.0 cm；生根培养基为 1/2WPM+IBA 1.5 mg∙L-1+5-NGS 4.5 mg∙L-1+蔗糖 20.0g∙L-1，培养 40 d，最高生根率 83.3%；生根苗较适合的移栽基质为泥碳土，较好的移栽季节为 3—5 月和10—11 月，移栽后在遮光度 70%的大棚中培养 40 天，移栽成活率 54.3%~65.6%。
Subjects: Biology >> Botany >> Applied botany submitted time 2018-10-26 Cooperative journals: 《广西植物》
Abstract：以广西野生金线莲无菌播种离体茎段为材料，采用单因素对比试验，研究植物激素（NAA、IBA、6-BA、GA3、KT、ZT、TDZ、2-IP）以及接种方式（竖直接种和水平接种）对壮苗生根培养的影响。结果表明，与对照相比，生长素有利于壮苗生根，NAA 的效果优于 IBA；细胞分裂素对壮苗生根的效果依次为 6-BA>TDZ>KT=ZT>2-IP>CK，其中 6-BA 诱导平均株高8.4 cm，3.6 条根，茎粗为 2.84 mm，植株生长健壮，诱导效果最好；赤霉素 GA3诱导出的植株高且直，但植株细弱，且抑制根系生长，不利于壮苗生根培养；在激素组合 6-BA 0.5 mg·L-1、NAA1.0 mg·L-1 处理中，组培苗生长健壮且根数量较多，效果最佳；水平接种能诱导出更多的根系，且便于接种操作，可以节省接种时间。因此，确定广西金线莲最适宜的壮苗生根培养基配方为 1/2MS+6-BA 0.5 mg·L-1+NAA 1.0 mg·L-1+香蕉泥 100 g·L-1+AC（活性炭）1.0 g·L-1+蔗糖 20 g·L-1，最佳接种方式为水平接种。
Subjects: Biology >> Botany >> Applied botany submitted time 2018-09-07 Cooperative journals: 《广西植物》
Abstract： TissuecultureandrapidpropagationtechniqueofCalotropisgiganteaL.wasstudied byusingstemsegmentsasexplantsinthispaper.Theresultsshowedthattheoptimalsterilization 32.3%. The suitable primary induction medium was MS+ sucrose 30g稬-1+ ager 3.5 g稬-1. And after 20 d culture, 3-4 cm high regenerative buds were formed. Pre-multiplication culture test showed that MS+ 6-BA1.5 mg稬-1+ NAA0.05 mg稬-1+AgNO31.0 g稬-1+ sucrose 30 g稬-1+ ager 3.5 g稬-1 was a suitable multiplication medium, proliferation coefficient was 4.6/25 d. However, during the subsequent cultivation process, using this method the seedlings were easy to vitrify. With the change of generation, the degree of vitrification has been increasing. So inhibition of vitrification was the key to success.Based on the above multiplication culture test, withAgNO3 as a vitrification inhibitor, the suitable multiplication culture medium of MS+ 6-BA 1.5 mg稬-1+ NAA 0.05 mg稬-1+ AgNO3 1.0 g稬-1+ sucrose 30 g稬-1+ ager 3.5 g稬-1 was verified. With this method, 25 d later, seedling height was 5-8 cm, proliferation coefficient was higher than 5.8/25 d and vitrification rate of multiple shoots was lower than 10%.The optimal medium for rooting was 1/2MS+ NAA1.0 mg稬-1+ sucrose 20 g稬-1 + ager 3.6 g稬-1, 14 d later, the rooting rate was 98%. The rooting seedlings were transplanted in the 70% shading greenhouse.And 30 d later,they were about 20 cm high, with 85% survival rate. This method can be used for the large-scale production ofexcellentclonalseedlingsofCalotropisgiganteaL.
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