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Your conditions: 2018-7
  • 全球免疫细胞治疗药物开发现状与趋势

    Subjects: Biology >> Bioengineering submitted time 2018-07-31 Cooperative journals: 《中国生物工程杂志》

    Abstract:目的:从产品开发角度分析免疫细胞治疗类药物的发展现状和未来趋势。方法:检索科睿唯安(Clarivate Analytics)的Cortellis数据库的数据,利用定量分析法和对比分析法对检索结果进行分析。结果:目前已有2种免疫细胞治疗类药物上市,1种免疫细胞治疗类药物处于预注册阶段,4种药物处于临床III期,同时大量处于临床II/I期药物显示未来市场上将有更多免疫细胞治疗类药物。产品交易方面,目前在免疫细胞治疗类药物的商业交易也趋向频繁。目前已发生的交易中,本文列举分析了交易金额前十的交易,其中药物开发及商业化许可是最主要的交易模式。结论:目前免疫细胞治疗类药物市场尚处于起步阶段,但随着未来技术的不断发展改进,相信未来有更多的药物进入商用市场,为癌症及其他疾病的治疗提供新的契机。

  • 黔中石漠化区衰老退化与正常生长顶坛花椒根区土壤质量特征

    Subjects: Biology >> Botany >> Applied botany submitted time 2018-07-23 Cooperative journals: 《广西植物》

    Abstract: As the aging and degradation of Zanthoxylum planispimum var. dintanensis has affected the rocky desertification control, and in order to discuss the reason of aging and degradation of Zanthoxylum planispimum var. dintanensis, and realize the stabilization of forest stand and sustainable management, this paper took aging degraded plants with typical symbol of yellow blooms and normally grown plants as the research objects and adopted soil agrochemistry and environmental mineralogy methods to analyze mineral element content in root-zone soil of different plants, evaluate integrated soil quality and to reveal the mineral element mechanism of aging and degradation of Zanthoxylum planispimum var. dintanensis. The results shows that (1) C, P, K, S, Se, Sr, Mo and oxide in root-zone soil of normally grown plants were generally higher than the content in aging degraded plants, while total nitrogen, rapidly available phosphorus, available nitrogen, Cu, Pb, Zn and Cr had no significant difference, and other elements had no obvious change rule. It indicates that mineral elements are one of the causes of the aging and degradation of Zanthoxylum planispimum var. dintanensis. Available contents of elements also had an impact on plant growth, which indicates that we should improve availability of nutrients when managing the Zanthoxylum planispimum var. dintanensis. From the perspective of plant nutrition demand, all major, medium and micro elements had a dominant effect on soil quality. In the management of soil fertility, we should not only focus on a large number of elements. (2) Root-zone soil quality indexes manifested that the highest plant was No. 3 normally grown plant and the lowest plant was No. 1 aging degraded plant, suggesting that soil quality influences the aging and degradation of Zanthoxylum planispimum var. dintanensis. But the soil quality is not the only cause of aging degradation of Zanthoxylum planispimum var. dintanensis, and more comparative studies of soil microbes and plant hormones should be conducted. (3) When managing the Zanthoxylum planispimum var. dintanensis in forest stand, cultivate good soil structure, make sure the comprehensiveness and proportionality of soil nutrient, and pay attention to deficit effect caused by low mineral elements, and it is necessary to make sure the appropriate nutrient concentration and ratio to increase the absorption capacity of the plant.

  • 植物生态化学计量内稳性特征

    Subjects: Biology >> Botany >> Applied botany submitted time 2018-07-23 Cooperative journals: 《广西植物》

    Abstract: Stoichiometric homeostasis is one of the core concepts of ecological stoichiometry. It refers to the ability of an organism to maintain a given elemental composition in the body despite variation in the elemental composition of its environment or diet. It reflects the net outcome of many underlying physiological and biochemical adjustments as organisms respond to their surroundings. The homeostatic index (H) of plant can be estimated by measuring the nitrogen and phosphorus contents and N:P ratio in plant and soil. In general, the homeostasis of plants is weaker than that of animals, with a larger variety of homeostasis. Stoichiometric homeostasis is an important mechanism in maintaining ecosystem structure, function, and stability. The homoeostatic species tend to have high and stable biomass; and ecosystems dominated by more homoeostatic species have higher productivity and greater stability. Therefore, the homeostasis is considered to be an important index to measure species competitiveness. Studies of stoichiometric homeostasis of plant will help us to understand the adaptation strategies and ecological adaptability of plants to the environment, as well as the relationship between plant ecological stoichiometry homeostasis and ecosystem functions. However, there are few studies on the internal stability of plant ecological stoichiometry. Previous research showed that the stoichiometric homeostasis characteristics of different plant species or functional groups were different. There were differences in stoichiometric homeostasis among different growth stages, different organs of the same species, as well as different nutrient elements. This paper reviews the concept of stoichiometric homeostasis, the estimation of plant homeostatic index (H), the stoichiometric homeostasis characteristics of different plant species or functional groups, different organs and growth stages and different elements, as well as linking plant stoichiometric homoeostasis with ecosystem structure, functioning and stability. Based on the obtained achievements and the current study, we put forward some perspectives of plant stoichiometric homoeostasis for future research to be conducted with an aim to promote this discipline of research in China.

  • ‘宁杞 8 号’体细胞胚胎发生体系建立

    Subjects: Biology >> Botany >> Applied botany submitted time 2018-07-23 Cooperative journals: 《广西植物》

    Abstract: In order to establish a high-frequency regeneration system for Lycium barbarum. The leaf of Lycium barbarum ‘Ningqi 8’ was selected as materials, to explore the crucial effects of hormone combinations and additives on the induction of somatic embryogenesis, germination and regeneration. The results showed that By orthogonal analysis of 6-BA, 2,4-D and IAA, the optimal hormone combination of ‘Ningqi 8’ somatic embryos was 6-BA 1.0 mg・L-1+2,4-D 0.3 mg・L-1+IAA 0.4 mg・L-1, the highest induction rate could get to 88.67%. Range analysis showed that 6-BA had the most significant effect on somatic embryogenesis. Using suitable proportion of hormone combination was possible to induce somatic embryogenesis of ‘Ningqi 8’, which had a large number of normal morphology. In the culture of somatic embryos, the high concentration of 6-BA was easy to cause vitrification, which was not conducive to the growth and proliferation of ‘Ningqi 8’ somatic embryos. The proliferation rate of somatic embryos increased with the increase of hormone concentration, but the vitrification rate increased too. Then the most efficient proliferation medium for ‘Ningqi 8’ was 6-BA 0.4 mg・L-1+NAA 0.6 mg・L-1, and the highest germination rate of ‘Ningqi 8’ somatic embryo was obtained when IBA 0.3 mg・L-1+GA3 0.4 mg・L-1+ sucrose 10g・L-1 was added. The highest germination rate could get to 89.17%, and the degree of influence on the germination of ‘Ningqi 8’ was IBA > sucrose > GA3. Under the condition of adding GA3 and low concentration sucrose could promote the mature somatic embryos germination. Activated carbon could effectively improve the regeneration rate of ‘Ningqi 8’ somatic embryo, and simultaneously promote the development of the root of germination embryo. The IBA 0.1 mg・L-1+KT 0.4 mg・L-1+ activated carbon 1g・L-1 was found to be suitable medium for regeneration in which ‘Ningqi 8’ demonstrated a highest multiplying capacity, and the regeneration rate could get to 91.67%. The research provides a new avenue for the improvement of Lycium barbarum varieties and breeding of new varieties.

  • New progress on the correlation of Chinese terrestrial Permo-Triassic strata

    Subjects: Biology >> Other Disciplines of Biology submitted time 2018-07-18 Cooperative journals: 《古脊椎动物学报》

    Abstract: This paper reviews recent works on the age of Chinese terrestrial Permo-Triassic strata, mainly based on the isotopic dating and the tetrapod correlation. For the application of U-Pb dating methods for the stage-level division of Permo-Triassic strata, the best method is the Thermal Ionization Mass Spectrometer (TIMS), while the Secondary Ion Mass Spectroscopy (SIMS) can be used for many cases; however, the most popular Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICPMS), which has nearly 4% error (>8 Myr), is of little use (Li et al., 2015).

  • New ootype prismatoolithids from the Late Cretaceous, Laiyang Basin and its significance

    Subjects: Biology >> Other Disciplines of Biology submitted time 2018-07-18 Cooperative journals: 《古脊椎动物学报》

    Abstract: Here we discribe a new ootypes of prismatoolithids found from the Late Cretaceous Jiangjunding Formation in the Laiyang Basin, Shandong Province. On the basis of characters such as elongate ovoids, smooth eggshell surface, relatively thinner eggshell thickness, prismatic eggshell units, slender pores in radial section of eggshell and little pores with round or irregular shapes in tangeital section of eggshell, we erect one new oogenus and one new oospecies: Laiyangoolithus lixiangensis oogen. et oosp. nov. The discovery of L. lixiangensis not only enriches the diversity and composition of the Laiyang Dinosaur Egg Fauna, but the paleogeographic distribution of primatoolithids as well. In addition, it provides more paleontological materials for the study of the diversity and paleogeographic distribution of troodontids in China.

  • 钙库调控的钙通道Orai1体外研究方法的建立

    Subjects: Biology >> Bioengineering submitted time 2018-07-15 Cooperative journals: 《中国生物工程杂志》

    Abstract:目的:建立钙通道Orai1的体外研究方法。方法:利用脂质体重组技术,将体外纯化的Orai1蛋白重组到脂质体膜上,并利用蔗糖密度梯度离心来检测其重组效率及Orai1蛋白在脂质体膜上的结构,并利用钙染料Fura-2检测脂质体内钙离子的释放。结果:成功制备了脂质体及体外纯化了GST-Orai1融合蛋白,蔗糖密度梯度离心结果证明GST-Orai1蛋白成功重组到脂质体上,以及Orai1蛋白以多聚体的形式定位在脂质体膜上。钙离子释放实验证明脂质体内钙离子包装完好,可用于后续Orai1钙通道的功能研究。结论:利用脂质体重组技术建立了一种新的Orai1的研究方法,能够更直接有效地研究其功能及其活化机制。

  • CRISPR/Cas系统作为抗菌药的现状及展望

    Subjects: Biology >> Bioengineering submitted time 2018-07-15 Cooperative journals: 《中国生物工程杂志》

    Abstract:抗生素长期滥用导致了人体内菌群失调及细菌耐药性的产生,因此需要寻找新型、靶向抗菌方法来治疗耐药细菌的感染。近年来,CRISPR/Cas系统的深入研究为设计特异性靶向耐药基因,定向清除耐药细菌的药物提供了新的思路。在此,本文介绍了CRISPR/Cas系统作为新型抗菌方法,通过靶向切割抗性质粒或细菌基因组以实现对耐药基因或病原菌的特异性清除,并对CRISPR抗菌药的不同类型核酸酶的选择,以及CRISPR递送系统的运载工具进行了评价。

  • 利用CRISPR/Cas9技术构建AEG-1基因敲除U251细胞系并探讨其转移行为的特点

    Subjects: Biology >> Bioengineering submitted time 2018-07-15 Cooperative journals: 《中国生物工程杂志》

    Abstract:星形胶质细胞上调基因-1(astrocyte elevated gene-1,AEG-1)在多种肿瘤中过表达,参与肿瘤的形成、转移等过程。本实验利用CRISPR/Cas9技术敲除AEG-1基因并研究其在胶质瘤细胞转移过程中的作用。首先设计构建sgRNA/Cas9二合一表达载体并转染到人胶质瘤U251细胞中,通过TA克隆测序鉴定sgRNA的活性;然后筛选建立稳定的AEG-1敲除U251细胞系,并利用Western blot实验检测AEG-1的敲除效率;最后利用Transwell小室、划痕实验评价AEG-1敲除后对肿瘤细胞迁移能力的影响。结果显示,成功构建靶向敲除AEG-1基因的sgRNA/Cas9二合一表达载体,所构建的载体与实验设计相一致,通过TA克隆测序鉴定sgRNA有活性;成功建立稳定的AEG-1敲除U251细胞系,Western blot实验结果表明敲除效率高达98%;Transwell小室实验、划痕实验结果表明AEG-1敲除U251细胞系的转移能力明显降低。

  • 脐带间充质干细胞的发现研究及规模化生产

    Subjects: Biology >> Bioengineering submitted time 2018-07-15 Cooperative journals: 《中国生物工程杂志》

    Abstract:脐带间充质干细胞来源于人体脐带组织,具有自我复制更新和多向分化潜能,是一种具有许多优势的干细胞类型,可用于治疗老年性退变、帕金森、血管缺血、小儿自闭症、系统性红斑狼疮、强直性脊柱炎、多发性神经硬化症、糖尿病、各种创伤、移植物抗宿主病等常规治疗方法难以治愈的疾病,并且治疗效果明确。本文介绍了脐带间充质干细胞的研究发现历程,临床应用价值,分离制备和检测鉴定方法,生物学特性以及规模化生产技术。需要强调的是,脐带间充质干细胞具有比其他一些干细胞(如脐带造血干细胞、骨髓造血干细胞、骨髓间充质干细胞等)没有的优势,材料来源广泛,细胞增殖能力强,没有伦理问题。脐带间充质干细胞的生产和应用应遵循政府或机构颁布的相关法律法规和标准规范。

  • S100A9参与乙型肝炎病毒X蛋白介导的HepG2细胞增殖与迁移

    Subjects: Biology >> Bioengineering submitted time 2018-07-11 Cooperative journals: 《中国生物工程杂志》

    Abstract:目的:探讨S100A9在乙型肝炎病毒X(HBx)介导的HepG2细胞增殖及迁移中的作用。方法:用表达HBx蛋白的重组腺病毒AdHBx感染HepG2细胞后,用CCK-8实验检测细胞增殖能力及划痕愈合实验检测细胞迁移能力;在HepG2/AdHBx细胞中转染S100A9-siRNA及其对照siRNA后,检测HepG2细胞增殖及迁移能力;在HepG2/AdHBx和对照组HepG2/AdGFP细胞中,采用Real- time PCR及Western Blot检测S100A9基因及蛋白的表达情况;在HepG2/AdHBx细胞中,加入不同剂量的NF-κB抑制剂BAY11-7082后,检测各组中S100A9的基因及蛋白表达情况。结果:HBx促进HepG2细胞的增殖与迁移;S100A9-siRNA抑制S100A9的表达后,HBx促进HepG2细胞的增殖与迁移的作用降低,HBx介导的HepG2细胞的增殖与迁移部分依赖于S100A9;S100A9基因及蛋白表达在HepG2/AdHBx中较对照组HepG2/AdGFP显著升高,HBx可致S100A9表达增加;抑制NF-κB转录活性后,AdHBx+BAY11-7082组S100A9基因及蛋白表达较对照组显著降低,阻断NF-κB转录活性可部分抑制HBx调控的S100A9表达。结论:HBx可调控S100A9的表达且与NF-κB活化有关, S100A9参与 HBx介导的HepG2细胞的增殖与迁移。

  • 地高辛标记Ucp2 基因RNA探针的制备和应用

    Subjects: Biology >> Bioengineering submitted time 2018-07-11 Cooperative journals: 《中国生物工程杂志》

    Abstract:目的:为了制备用于检测小鼠胚胎早期Ucp2基因表达的地高辛标记的特异性RNA探针。方法:提取小鼠胚胎脑组织总RNA,设计引物,通过RT -PCR方法获取Ucp2基因片段,将其克隆到pGEM -T载体。分别利用Sp6、T7和Ucp2特异性引物,PCR扩增获得转录模板,通过Sp6及T7 RNA聚合酶,获得地高辛标记的正义、反义Ucp2 RNA原位杂交探针。检测标记探针的效价后,通过全胚胎原位杂交分析制备探针的特异性和杂交效果。结果:成功获得Ucp2基因正义、反义探针,反义探针能高效灵敏检测到Ucp2基因在小鼠胚胎Ed9.5、Ed10.5神经系统呈现高表达,而正义探针未能检测到表达信号。结论:成功制备了特异高效的地高辛标记Ucp2 RNA 原位杂交探针,为进一步研究Ucp2基因在小鼠胚胎组织中的表达,尤其在神经组织的定位奠定基础。

  • 华氏巨球蛋白血症相关突变MYD88 L265P新型检测体系的建立

    Subjects: Biology >> Bioengineering submitted time 2018-07-06 Cooperative journals: 《中国生物工程杂志》

    Abstract:华氏巨球蛋白血症(Waldenström’s macroglobulinemia,WM)是一种罕见的,不可治愈的淋巴浆细胞淋巴瘤(lymphoplasmacytic lymphoma,LPL)。MYD88 L265P突变在华氏巨球蛋白血症患者中检出率很高(>90%),可以用于疾病的鉴别和诊断,因此,开发一种高灵敏度的检测方法对这个突变进行检测具有较大的临床价值。通过将ARMS技术与Clamping PCR技术相结合,建立的新型MYD88 L265P突变富集检测体系可以满足这一需求。优化后,该检测体系检出限为102拷贝,灵敏度为0.1%,对19份临床样品的双盲试验中,检测结果准确率达到100%。所建立的方法具有灵敏、准确的优势,适用于华氏巨球蛋白血症的早期诊断,有较为广阔的应用前景。

  • 环介导等温扩增技术检测食品安全的研究进展

    Subjects: Biology >> Bioengineering submitted time 2018-07-01 Cooperative journals: 《中国生物工程杂志》

    Abstract: 食品安全一直是全世界公众健康的关注焦点。环介导等温扩增(Loop-mediated isothermal amplification,LAMP)技术是一种特异性强、灵敏度高、快速简便的等温核酸扩增技术,近年来在核酸检测领域有着广泛的研究和应用。将LAMP技术应用到食品安全检测领域,可以快速准确的监控一些食品安全问题对人类健康所构成的危害。因此针对LAMP技术在食品安全检测方面的优势进行分析,并结合LAMP技术与新兴诊断技术平台的联合运用进行了展望。

  • 铜绿假单胞菌Ⅵ型分泌系统的研究进展

    Subjects: Biology >> Bioengineering submitted time 2018-07-01 Cooperative journals: 《中国生物工程杂志》

    Abstract: 铜绿假单胞菌是一种能引起多部位急慢性感染且难以用抗生素控制的机会致病菌,近年来已成为院内感染的主要致病菌之一。大量研究表明,细菌将毒力因子精准输送至宿主细胞是其致病的关键,分泌系统在这一过程中扮演重要作用,其中近期发现的Ⅵ型分泌系统(Type VIsecretion system,T6SS)在铜绿假单胞菌与宿主间的相互作用和促进生物膜的形成等机制中发挥重要作用,已引起国内外学者高度关注。着重对铜绿假单胞菌T6SS的结构组成、效应功能和调节机制等相关研究进行简要综述,旨在为铜绿假单胞菌感染患者的治疗提供新策略。