Subjects: Biology >> Botany >> Applied botany submitted time 2021-04-29 Cooperative journals: 《广西植物》
Abstract： Traditionally, patchouli had been divided into three cultivations, including Pogostemon cablin cv. shipaiensis, P. cablin cv. gaoyaoensis and P. cablin cv. zhanjiangensis. In order to explore the effect of the interaction between the endophytic fungi and the patchouli on the formation mechanism of active components, a laboratory experiment was conducted for investigating the endophytic fungi of P. cablin cv. shipaiensis and P. cablin cv. zhanjiangensis with chemical composition difference. The strains were classified by traditional morphological methods firstly. Then the rDNA-ITS sequences of the strains were amplified by the common fungal primer ITS1/ITS4 to identify their taxonomic status and their diversity. The results showed that 3 070 strains were isolated from the stem and leaf tissues of patchouli in seedling, branching and adult stages by PDA and LBA culture media. The 1 624 strains of endophytic fungi from P. cablin cv. shipaiensis were isolated and 1 319 strains were identified, which belonged to 36 genera. There were seven endophytic fungi that were not found in P. cablin cv. zhanjiangensis, including Epichloe typhina, Colletotrichum gloeosporioides, Botryosphaeria sp., Rhizoctonia sp., Truncatella sp., Phytophthora sp. and Sclerophthora sp. For the first time, the endophytic fungi of Oomycete were isolated. There were 1 446 strains isolated from P. cablin cv. Zhanjiangensis，and 994 strains were identified, which were belonging to 33 genera. Among them, there were two endophytic fungi that were not found in P. cablin cv. shipaiensis, including Paecilomyces sp. and Cercospora sp. The dominant endophytic fungi were Alternaria sp. and Colletotrichum sp. in two kinds of cultivated patchouli. The relative separation frequencies of the Alternaria sp. and Colletotrichum sp. were 9.48% and 7.81% in P. cablin cv. shipaiensis, and were 10.16% and 8.65% in P. cablin cv. zhanjiangensis. The colonization rates of endophytic fungi gradually increased with the growth of patchouli from seedling to adult stages, which were Aug.(97.78%) > Jul.（72.50%）> May（55.28%）in P. cablin cv. shipaiensis, and Aug.(91.11%) > Jul.(63.06%) > May(46.67%) in P. cablin cv. zhanjiangensis. The average colonization rates were 75.19% and 66.95%. There were a positive correlation between the growth periods and the shannon index, which increased with the growth of patchouli. The average sorenson similarity index was 0.86. Obviously, the two cultivated patchouli had a large population of endophytic fungi in the stems and leaves, but the community structure was significantly different. These results lay a foundation for screening active strains and exploring the effect of endophytic fungi on the synthesis and accumulation of active components of patchouli.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-04-29 Cooperative journals: 《广西植物》
Abstract： Traditionally, patchouli had been divided into three cultivations, including Pogostemon cablin cv. shipaiensis, P. cablin cv. gaoyaoensis and P. cablin cv. zhanjiangensis. In order to explore the effect of the interaction between the endophytic fungi and the patchouli on the formation mechanism of active components, a laboratory experiment was conducted for investigating the endophytic fungi of P. cablin cv. shipaiensis and P. cablin cv. zhanjiangensis with chemical composition difference. The strains were classified by traditional morphological methods firstly. Then the rDNA-ITS sequences of the strains were amplified by the common fungal primer ITS1/ITS4 to identify their taxonomic status and their diversity. The results showed that 3 070 strains were isolated from the stem and leaf tissues of patchouli in seedling, branching and adult stages by PDA and LBA culture media. The 1 624 strains of endophytic fungi from P. cablin cv. shipaiensis were isolated and 1 319 strains were identified, which belonged to 36 genera. There were seven endophytic fungi that were not found in P. cablin cv. zhanjiangensis, including Epichloe typhina, Colletotrichum gloeosporioides, Botryosphaeria sp., Rhizoctonia sp., Truncatella sp., Phytophthora sp. and Sclerophthora sp. For the first time, the endophytic fungi of Oomycete were isolated. There were 1 446 strains isolated from P. cablin cv. Zhanjiangensis，and 994 strains were identified, which were belonging to 33 genera. Among them, there were two endophytic fungi that were not found in P. cablin cv. shipaiensis, including Paecilomyces sp. and Cercospora sp. The dominant endophytic fungi were Alternaria sp. and Colletotrichum sp. in two kinds of cultivated patchouli. The relative separation frequencies of the Alternaria sp. and Colletotrichum sp. were 9.48% and 7.81% in P. cablin cv. shipaiensis, and were 10.16% and 8.65% in P. cablin cv. zhanjiangensis. The colonization rates of endophytic fungi gradually increased with the growth of patchouli from seedling to adult stages, which were Aug.(97.78%) > Jul.（72.50%）> May（55.28%）in P. cablin cv. shipaiensis, and Aug.(91.11%) > Jul.(63.06%) > May(46.67%) in P. cablin cv. zhanjiangensis. The average colonization rates were 75.19% and 66.95%. There were a positive correlation between the growth periods and the shannon index, which increased with the growth of patchouli. The average sorenson similarity index was 0.86. Obviously, the two cultivated patchouli had a large population of endophytic fungi in the stems and leaves, but the community structure was significantly different. These results lay a foundation for screening active strains and exploring the effect of endophytic fungi on the synthesis and accumulation of active components of patchouli.
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