Subjects: Biology >> Botany >> Applied botany submitted time 2022-07-05 Cooperative journals: 《广西植物》
Abstract： Liquidambar formosana is one of the important native tree species in Guangxi, which has high timber, ornamental and medicinal value. This study designs and develops EST-SSR markers of L. formosana based on data from the transcriptome sequencing. The purpose is to provide an effective and reliable molecular marker tool for studies of population genetics of this species, it is of great significance to the protection and utilization of all kinds of material resources of the tree. Simple sequence repeats were selected from the transcriptome data in L. formosana, and then primers were developed, and screened out by PCR amplification and polyacrylamide gel electrophoresis for high polymorphism, and the efficiency was tested by using 30 individuals from of a wild L. formosana population. The results were as follows: (1) A total 23 777 SSRs were obtained by searching SSR unigenes from transcriptome data. The repeat type of the SSR loci in L. formosana was dominated by mononucleotide repeats (46.54%). The highest proportion of SSR loci (72.36%) was between 5 and 12 times. (2) A total of 262 pairs of SSR primers were developed, and among them, 139 pairs amplified the target bands with a success rate of 53.1%, and 18 pairs of them that can be used to steadily obtain clear bands were finally identified. (3) The polymorphism detection showed that all sites had a high degree of polymorphism. The number of alleles, effective alleles, Shannon diversity index, observed heterozygosity and fixed index of the L. formosana populations ranged in 2-4, 1.112 8-2.609 6, 0.208 9-1.112 7 and 0.275 9-1.000 0, the average values were 2.333 3, 1.957 4, 0.708 5 and 0.722 6, respectively. In conclusion, the dominant SSR repeat type and repeat motif in L. formosama are basically the same as those in other species, the developed EST-SSR markers can meet the needs of population genetic studies of L. formosana, which provides abundant primers for the study of genetic diversity of L. formosana.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-07-20 Cooperative journals: 《广西植物》
Abstract： Albizia odoratissima is a unique rare timber tree specie in the south of China, and it is of great significance to carry out group genetics research on its germplasm resources, which is of great significance to the protection and utilization of all kinds of material resources of the tree. This study designs and develops EST-SSR markers of Albizia odoratissima based on the transcriptome sequencing results. In addition, Albizia procera, Albizzia falcata, Acacia melanoxylon, Erythrophloeum fordii and other related species were selected for analysis of interspecific generality. The results showed that among the 243 pairs of developed primers, 171 pairs could successfully amplify the target bands, and the effective amplification rate in Albizia odoratissima Albizia procera, Albizzia falcataria, Acacia melanoxylon and Erythrophloeum fordii was 63.79%, 33.75%, 45.68%, 41.56% and 14.81% respectively, and the polymorphism ratio in them was 23.87%, 12.20%, 9.01%, 3.96% and 2.78% respectively. There were 18 pairs of primers that could be commonly used among Albizia odoratissima, A. procera, A. falcataria, Acacia melanoxylon and Erythrophloeum fordii. There were 37 SSR polymorphism markers for Albizia odoratissima were obtained, 10 polymorphism markers for Albizia procera and A. falcataria, 4 polymorphism markers for Acacia melanoxylon, and there was 1 polymorphism mark of Erythrophloeum fordii. The developed EST-SSR markers can meet the needs of population genetic studies of Albizia odoratissima, and have good transferability and practicability in Albizia procera and A. falcataria and other related tree species. In conclusion, EST-SSR markers will provide a reliable research tool for genetic diversity evaluation of germplasm resources, fingerprint construction of breeding materials, and population mating system analysis of Albizia odoratissima, A. procera, A. falcataria, Acacia melanoxylon and Erythrophloeum fordii.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-05-27 Cooperative journals: 《广西植物》
Abstract： Pinus taiwanensis var. damingshanensis is a kind of endemic Alpine pine of Guangxi and Guizhou, which has high economic and ecological value. Its natural population has not been fully protected and utilized for a long time, which is not conducive to long-term stable development of this species. In order to protect and exploit the natural genetic resources of Pinus taiwanensis var. damingshanensis, 12 SSR markers were used to study the genetic diversity of three natural populations of Pinus taiwanensis var. damingshanensis, and to analyze the genetic differentiation and gene flow among populations, so as to provide reference for the protection strategy of this species. The results were as follows: 37 alleles were detected by 12 pairs of primers, and the percentage of polymorphic loci was 100%. For every site, the average number of observed alleles (Na) was 3.08, and the average number of effective alleles (Ne) was 1.68. The number of effective alleles varied greatly among different loci. The average observed heterozygosity (Ho) was 0.35, the average expected heterozygosity (He) was 0.40, and the average polymorphism information content (PIC) was 0.31 for every site. The Shannon's diversity index of the three populations ranged from0.48 to 0.65, and the Nei's gene diversity ranged from0.27 to 0.39. Compared with other related species of pines, the genetic diversity was low. For each population, the average observed heterozygosity was 0.40, and the average expected heterozygosity was 0.33 ， the average number of effective alleles was 1.58. The genetic differentiation coefficient (Gst) among populations was 0.10. Most of the variation existed in the population and the genetic differentiation level among populations was low. The range of gene flow (Nm) was 2.74, indicating that gene exchange between populations was sufficient of Pinus taiwanensis var. damingshanensis. This study can provide an important reference for the protection of biodiversity, and lay a foundation for the scientific utilization of Pinus taiwanensis var. damingshanensis.
Subjects: Biology >> Botany >> Applied botany submitted time 2020-03-06 Cooperative journals: 《广西植物》
Abstract：探究不同松树种间杂交可育性，为松树种间杂交育种亲本选配提供依据，进而为松树的杂种优势研究积累材料。本研究利用随机区组设计，通过92 个松树种间杂交组合与20个半同胞对照，分析9 种松树种间杂交类型子代的平均球果产种数，平均球果产种量，百粒重，发芽率与成苗率5 项育性指标，综合球果、种子与苗期三方面的表现，利用模糊数学隶属函数法对不同松树种间杂交类型进行可育性综合评价。分析得出：加勒比松×湿地松（C×E）、湿地松×加勒比松（E×C）与湿地松×火炬松（E×T）三种杂交类型育性表现优于或接近相应的半同胞对照，马尾松×湿地松（M×E）、马尾松×火炬松（M×T）、马尾松×加勒比松（M×C）、火炬松×加勒比松（T×C）、火炬松×马尾松（T×M）与火炬松×湿地松（T×E）杂交类型育性表现远低于半同胞对照，表现出低育性甚至不育。综合5 项育性指标，亚组内杂交育性的整体表现明显优于亚组间杂交；湿地松与加勒比松为母本的三种杂交组合育性表现总体水平明显优于马尾松与火炬松为母本的另外六种杂交组合。9 种杂交类型中除了火炬松×湿地松（T×E）外，均获得了具有生活力的杂交子代，尤其马尾松与3 种国外松的杂交子代在相关研究中是首次获得，对松树种间杂交育种的研究具有重要意义。
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