Subjects: Biology >> Botany >> Applied botany submitted time 2022-07-05 Cooperative journals: 《广西植物》
Abstract： Liquidambar formosana is one of the important native tree species in Guangxi, which has high timber, ornamental and medicinal value. This study designs and develops EST-SSR markers of L. formosana based on data from the transcriptome sequencing. The purpose is to provide an effective and reliable molecular marker tool for studies of population genetics of this species, it is of great significance to the protection and utilization of all kinds of material resources of the tree. Simple sequence repeats were selected from the transcriptome data in L. formosana, and then primers were developed, and screened out by PCR amplification and polyacrylamide gel electrophoresis for high polymorphism, and the efficiency was tested by using 30 individuals from of a wild L. formosana population. The results were as follows: (1) A total 23 777 SSRs were obtained by searching SSR unigenes from transcriptome data. The repeat type of the SSR loci in L. formosana was dominated by mononucleotide repeats (46.54%). The highest proportion of SSR loci (72.36%) was between 5 and 12 times. (2) A total of 262 pairs of SSR primers were developed, and among them, 139 pairs amplified the target bands with a success rate of 53.1%, and 18 pairs of them that can be used to steadily obtain clear bands were finally identified. (3) The polymorphism detection showed that all sites had a high degree of polymorphism. The number of alleles, effective alleles, Shannon diversity index, observed heterozygosity and fixed index of the L. formosana populations ranged in 2-4, 1.112 8-2.609 6, 0.208 9-1.112 7 and 0.275 9-1.000 0, the average values were 2.333 3, 1.957 4, 0.708 5 and 0.722 6, respectively. In conclusion, the dominant SSR repeat type and repeat motif in L. formosama are basically the same as those in other species, the developed EST-SSR markers can meet the needs of population genetic studies of L. formosana, which provides abundant primers for the study of genetic diversity of L. formosana.
Subjects: Biology >> Botany >> Applied botany submitted time 2018-10-26 Cooperative journals: 《广西植物》
Abstract：海南龙血树是国产血竭的主要基源植物，其血竭主要化学成分为类黄酮化合物。目前对血竭的研究集中在化学成分及药理活性分析，对血竭形成的分子机制并不了解。WD40转录因子在类黄酮的积累中具有重要的调控作用。本研究根据海南龙血树转录组数据，利用RT-PCR技术在海南龙血树中克隆了一个WD40基因DcWD40-1，该基因包含一个1 353 bp的开放阅读框，编码450个氨基酸，蛋白质分子量50.77 kD，理论等电点5.71。生物信息学分析显示，DcWD40-1属于WD40蛋白家族成员，具有5个保守的WD40结构域，和其他植物WD40蛋白同源性高，保守性强。利用Genome Walking方法分离了1 503 bp的DcWD40-1启动子序列，该区域具有典型真核生物启动子结构特征，并含有多个应答激素和胁迫的响应元件。表达分析显示，血竭诱导剂能够诱导DcWD40-1的表达，DcWD40-1的变化与血竭形成及类黄酮积累正相关。此外，DcWD40-1也能对茉莉酸甲酯、细胞分裂素、油菜素内酯和UV-B处理做出积极响应。本结果为进一步研究DcWD40-1在类黄酮生物合成中的潜在功能和作用机制奠定基础。
Subjects: Biology >> Zoology submitted time 2017-10-10 Cooperative journals: 《动物营养学报》
Abstract：本文旨在比较分析高、低脂系肉鸡肌肉品质的差异。以东北农业大学培育的高、低脂系第20世代肉鸡为试验动物，采用常规肉品质检测方法，测定肌肉的物理性状、化学性状、组织学特性以及风味物质，并比较这些性状在两系间的差异。结果表明：1）物理性状方面，高脂系肉鸡胸肌的蒸煮损失和滴水损失显著或极显著高于低脂系肉鸡（P<0.05或P<0.01），而胸肌的肉色亮度值（45 min）和黄度值（45 min和24 h）、pH24 h、失水率和剪切力显著或极显著低于低脂系肉鸡（P<0.05或P<0.01）；高脂系肉鸡腿肌的pH24 h、滴水损失、蒸煮损失和剪切力极显著高于低脂系肉鸡（P<0.01），而腿肌的肉色亮度值（45 min）和黄度值（45 min和24 h）、pH45 min和失水率显著或极显著低于低脂系肉鸡（P<0.05或P<0.01）。2）化学性状方面，高脂系肉鸡胸肌和腿肌的水分和粗蛋白质含量显著或极显著高于低脂系肉鸡（P<0.05或P<0.01），而粗脂肪含量极显著低于低脂系肉鸡（P<0.01）。3）组织学特性方面，高脂系肉鸡胸肌的肌纤维直径显著高于低脂系肉鸡（P<0.05），腿肌的肌纤维密度极显著高于低脂系肉鸡（P<0.01）。4）风味物质方面，高脂系肉鸡胸肌的甜鲜味氨基酸、必需氨基酸和总氨基酸含量显著或极显著高于低脂系肉鸡（P<0.05或P<0.01）；高脂系肉鸡胸肌和腿肌的多不饱和脂肪酸和必需脂肪酸含量极显著低于低脂系肉鸡（P<0.01）。总体而言，高、低脂系肉鸡肌肉的理化性状、组织学特性和风味物质含量差异显著，说明长期对腹脂性状的双向选择会在一定程度上影响肉鸡的肌肉品质。
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