Subjects: Biology >> Botany >> Applied botany submitted time 2022-03-18 Cooperative journals: 《广西植物》
Abstract： In order to explore the relationship between the growth of Paris polyphylla var. chinensis and the microbial community structure changes of the rhizosphere soil, the Illumina high-throughput sequencing technique was used to sequence the 16S rRNA gene of bacteria and 18S sequences of fungal at four different growth years, including 3 years, 5 years, 7 years and 9 years. The results were as follows: (1) Proteobacteria, Acidobacteria, Actinobacteria and Chloroflexi were dominant bacteria in the rhizosphere soil of P. polyphylla in different growth years. The dominant fungi phylas were Ascomycota, Basidiomycota and Mucoromycota. (2) The bacterial species in the rhizosphere of Paris polyphylla in different growth years were more abundant than fungi, and the degree of diversification was higher. The lowest point of bacterial diversity, which decreased, increased, and then decreased again with the increase of growth years showed a pattern of “И”, was at the 5th year and the highest point was at the 7th year. The fungal diversity showed a pattern of “ Λ ” that first increased and then decreased with the increase of growth years, and the highest point was at the 7th year. The abundance of bacterial communities also showed a pattern of “Λ” that first increased and then decreased with the increase of growth years, and the highest point was also at the 7th year. The abundance of fungi did not change much as the year growing. (3) The UPGMA cluster analysis showed that the microbial community structure of the rhizosphere soil had evolved significantly. The period when the bacterial community evolved more drastically was in the 7th year after planting, and the fungal community was in the 5th. (4) Spearman correlation analysis found that total nitrogen and available potassium were the main factors affecting the composition of rhizosphere soil bacteria, and Total potassium was the main factor affecting the composition of fungi. All the above results indicate that the composition and structure of the soil microbial community in the rhizosphere of wild P. polyphylla at different growth and development stages are different, and the 5-7th year is a critical period for significant changes in the diverdity of the soil microbial community.
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