Subjects: Biology >> Botany >> Applied botany submitted time 2021-04-13 Cooperative journals: 《广西植物》
Abstract： To establish the method for determination of gallic acid and catechin in the roots of Rosa laevigata(R. laevigata), and analyze the difference in the contents of gallic acid and catechin in roots of R. laevigata from different habitats and different processed methods resources from Guangxi. The contents of gallic acid and catechin of R. laevigata was used as evaluation index, the samples were determined by HPLC, and the data were analyzed by the analysis of variance (ANOVA) and discrimination study carried out based on the hierarchical clustering analysis (HCA) with SPSS 23.0 software. The results were as follows: The contents of gallic acid and catechin in roots of R. laevigata from different habitats and processed methods were different, the content of catechin was higer than that of gallic acid among all sample, the contents of gallic acid and catechin in roots of R. laevigata from the southern habitats (except Guiping, Guigang) were generally higher than those from northern habitats, the contents of gallic acid and catechin were highest in vinegar-processed products. The conclusion displayed that the differences of the contents of gallic acid and catechin in in roots of R. laevigata were mainly the differences of producting habitats and processed methods, the HPLC method is simple and feasible, which could provide a scientific basis for the resources development, quality control and clinical application of R. laevigata.
Subjects: Biology >> Botany >> Applied botany submitted time 2020-09-15 Cooperative journals: 《广西植物》
Abstract：建立广西金樱根药材中22种金属元素的电感耦合等离子体质谱( ICP-MS) 分析方法，并对不同产地药材及其炮制品中金属元素含量进行比较分析，以评价其药材的质量和优选适当的炮制方法。采用 ICP-MS 法测定22种金属元素含量，并绘制其特征金属元素柱状图谱，采用系统聚类分析和主成分分析对金樱根药材不同产地、不同炮制品的45个样品进行判别研究。结果表明：广西金樱根不同产地药材及其炮制品的45个样品中金属元素含量存在一定差异，其中Al元素含量偏高，经聚类分析和主成分分析将产地分为三类，主成分分析特征元素为Al，Pb，Ba，Zn，As，Sr。该研究通过金属元素在不同产地金樱根及炮制品中变化规律，表明金樱根醋炙的炮制方法最优，且可为金樱根的临床安全应用及质量控制提供理论依据和方法学参考。
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