Subjects: Biology >> Botany >> Applied botany submitted time 2021-04-29 Cooperative journals: 《广西植物》
Abstract： Solanum diphyllum is a kind of small shrub from Solanaceae, little is known about its Cd tolerance ability in the existed researches so far. In order to investigate the physiological responses, growth responses and Cd tolerance characteristics of S. diphyllum seedlings, we conducted a hydroponic experiment with 160 μM Cd stress to determine the indexes of growth (taproot, height, stem diameter, leaf number, biomass, and root to shoot ratio), tolerance ability, Cd content, Cd accumulation characteristics, SOD activity, osmotic adjustment substances (MDA, free proline and soluble sugar), and photosynthetic pigment (Chlorophyll a, Chlorophyll b and carotenoid) in different stress time (0, 0.5, 1, 3, 7, 19 d). The results showed that: compared with controls, Cd had no significant effect on the growth of S. diphyllum seedlings when stress time is 0-3 d, but obvious Cd toxicity was aggravated with the prolonging of Cd stress time (3-19 d). During the period of experiment, Cd stress reduced the total biomass of S. diphyllum seedlings by 13.28%-62.40%, root suffered the most serious biomass damage among different plant tissues. The maximum Cd accumulation of the whole plant was 60.14 μg·plant-1, for root and shoot the maximum Cd accumulation were 43.49 μg·plant-1 and 16.65 μg·plant-1 respectively, Cd accumulation of shoots accounted for 15.46%-35.24% of the whole plant. MDA content of S. diphyllum seedlings increased with increasing stress time, the maximum increase of MDA content in roots was 5.25 times than that of control. SOD activity, the content of free proline and soluble sugars, as well as photosynthetic pigments content showed a trend of increased in the beginning and then decreased. Tolerance index (TI) showed a different tendency, it decreased with increasing time, the minimum value of S. diphyllum seedlings during the whole period of experiment was 0.64. The above results showed that S. diphyllum seedlings have a certain degree of tolerance to high concentration of Cd stress (160 μM), which suggests it is a potential Cd tolerance plant. The osmotic adjustment and root retention might be the main methods of S. diphyllum seedlings to alleviated toxicity to plant growth caused by Cd. This research provides a theoretical basis for finding suitable phytoremediation materials.
Subjects: Biology >> Zoology submitted time 2017-10-23 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究过瘤胃保护甜菜碱（RPB）和过瘤保护胃胆碱（RPC）对1～3月龄湖羊生长性能、消化性能、屠宰性能和脂肪沉积的影响。试验选用30只体重相近的1月龄左右的湖羊公羔，随机分成3组，分别为对照组、RPB组和RPC组，每组10只。对照组饲喂基础饲料，RPB组和RPC组则饲喂在基础饲粮干物质（DM）基础上分别添加2.9 g/kg RPB和2.5 g/kg RPC的饲粮。预试期21 d，正试期54 d。结果表明：1）饲粮中添加RPB和RPC对1～3月龄湖羊的平均日增重、平均日采食量和料重比均无显著影响（P>0.05），但RPB组、RPC组的平均日增重较对照组分别增高了19.97%和27.75%。2）饲粮中添加RPB和RPC对1～3月龄湖羊的粗蛋白质（CP）、中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）、DM和有机物（OM）的表观消化率均无显著影响（P>0.05）。3）饲粮中添加RPB和RPC均可以显著降低1～3月龄湖羊的背膘厚（P0.05），但RPB组和RPC组眼肌面积较对照组分别增加了10.86%和3.16%，IMF含量较对照组分别增加了17.27%和36.36%，肾周脂肪重较对照组分别降低了20.60%和22.67%。综合以上试验结果得出，饲粮中添加2.9 g/kg RPB或2.5 g/kg RPC均可以改善1~3月龄湖羊的脂肪沉积部位和胴体品质，并且从消化性能、生长性能和屠宰性能来看，在满足维持需要的饲粮营养水平下，2种添加剂的作用相当。
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