Subjects: Biology >> Botany >> Applied botany submitted time 2024-03-26 Cooperative journals: 《广西植物》
Abstract： Andrographis paniculata is one of the most important “Southern Medicines” in China. It is used for clearing heat and detoxifying, cooling blood and reducing swelling. Its main active ingredient, andrographolide, functions in anti-cancer, anti-HIV, anti-inflammation and liver protection. It is difficult to be synthesized artificially, mainly relying on extraction from cultivated plant materials. However, the quality of cultivated medicinal materials is affected by various factors such as soil, climate, water and fertilizer management. On the other hand, A. paniculata has a long growth cycle, occupying land resources. The technology of plant in vitro culture has significant advantages in rapid propagation of seedling and accumulation of active ingredients, which is one of the important ways to achieve production of active ingredients rapidly and efficiently in A. paniculata. The in vitro regeneration technology system of A. paniculata is becoming more and more perfect, and the in vitro regeneration technology from explants to complete plants is becoming more and more mature, it has been applied in seedling propagation and ploidy breeding. At the same time, during callus culture, cell suspension culture, adventitic root culture and hairy root culture of A. paniculata, the accumulation of andrographolide and other active ingredients in the culture things could be greatly increased by optimizing the culture conditions and using appropriate inducers. This paper comprehensively and systematically reviewed the research advances in the technology of in vitro culture of A. paniculata and production of andrographolide from the aspects of tissue culture, cell culture, adventitious root culture, and hairy root culture. This paper aimed to provide reference for promoting the development and application of in vitro culture technology of A. paniculata, as well as for the study of in vitro production of andrographolide. It also put forward three aspects that need be focused on in future research on in vitro culture technology of A. paniculata and the production of andrographolides by this technology: (1) to mature and improve the tissues in vitro regeneration technology system of A. paniculata, and establish a comprehensive and systematic evaluation system; (2) to further increase the yield of andrographolide and other important active ingredients by optimizing the culture conditions and its combination with efficient inducers; and (3) to carry out researches in bioreactor culture of the production of andrographolide by cell suspension culture technology.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-02-07 Cooperative journals: 《广西植物》
Abstract： Andrographis paniculata is one of the most important “Southern Medicines” in China. It is used for clearing heat and detoxifying, cooling blood and reducing swelling. Its main active ingredient, andrographolide, functions in anti-cancer, anti-HIV, anti-inflammation and liver protection. It is difficult to be synthesized artificially, mainly relying on extraction from cultivated plant materials. However, the quality of cultivated medicinal materials is affected by various factors such as soil, climate, water and fertilizer management. On the other hand, A. paniculata has a long growth cycle, occupying land resources. The technology of plant in vitro culture has significant advantages in rapid propagation of seedling and accumulation of active ingredients, which is one of the important ways to achieve production of active ingredients rapidly and efficiently in A. paniculata. The in vitro regeneration technology system of A. paniculata is becoming more and more perfect, and the in vitro regeneration technology from explants to complete plants is becoming more and more mature, it has been applied in seedling propagation and ploidy breeding. At the same time, during callus culture, cell suspension culture, adventitic root culture and hairy root culture of A. paniculata, the accumulation of andrographolide and other active ingredients in the culture things could be greatly increased by optimizing the culture conditions and using appropriate inducers. This paper comprehensively and systematically reviewed the research advances in the technology of in vitro culture of A. paniculata and production of andrographolide from the aspects of tissue culture, cell culture, adventitious root culture, and hairy root culture. This paper aimed to provide reference for promoting the development and application of in vitro culture technology of A. paniculata, as well as for the study of in vitro production of andrographolide. It also put forward three aspects that need be focused on in future research on in vitro culture technology of A. paniculata and the production of andrographolides by this technology: (1) to mature and improve the tissues in vitro regeneration technology system of A. paniculata, and establish a comprehensive and systematic evaluation system; (2) to further increase the yield of andrographolide and other important active ingredients by optimizing the culture conditions and its combination with efficient inducers; and (3) to carry out researches in bioreactor culture of the production of andrographolide by cell suspension culture technology.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-05-27 Cooperative journals: 《广西植物》
Abstract： Andrographis paniculata, known as ‘Chuanxinlian’ in China, is an important southern medicine in China. Its main active ingredients, andrographolide components, has good pesticide effects on heat-clearing and anti-bacterial and anti-inflammatory. Modern medical research has demonstrated that they also have profound effects in anti-HIV, anti-thrombus, liver protection and so on. Because they are difficult to be synthesized artificially, to increase the biosynthesis of andrographolide components in A. paniculata by cultivation regulation and breeding methods is of great significance to improve the quality of this medicinal material. To provide reference for related research in these fields, this paper reviewed the relationship between accumulation of andrographolide components and plant development, the effects of cultivation measures and environmental factors on the accumulation of andrographolide components, and the research advances in biosynthesis of andrographolide components and its molecular regulatory mechanism in the last decade worldwide. It also put forward three aspects those should be focused on in future studies aiming at enhancing the biosynthesis and accumulation of andrographolides components in A. paniculata: (1) to further identify the biosynthetic pathway of andrographolide components and the functions of key genes, and to elucidate the mechanism of andrographolides accumulation at molecular level; (2) to deeply study the laws of growth and development, yield and quality formation, and their interrelations in A. paniculata with the combination of molecular biological approaches and crop cultivation theories; (3) to reveal the signal regulation network in the biosynthesis andrographolide components.
Subjects: Biology >> Zoology submitted time 2018-12-25 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究不同锌源（硫酸锌、蛋氨酸螯合锌和甘氨酸螯合锌）对湘东黑山羊生长性能及羊奶的成分、氨基酸和脂肪酸含量的影响。选取体重（38.1±9.7) kg、胎次相近的21只怀双羔妊娠湘东黑山羊（妊娠日龄在90~100 d），随机分为3组，即硫酸锌组（对照组）、蛋氨酸螯合锌组和甘氨酸螯合锌组。各组基础饲粮组成相同（锌含量22.00 mg/kg DM），并且各组锌添加量均为60 mg/kg DM。预试期为7 d，正试期为42 d。正试期3组母羊分别饲喂不同锌源饲粮，产后母羊饲喂无添加锌源的饲粮，母羊产后15 d采集奶样。结果表明：1）与对照组相比，蛋氨酸螯合锌组和甘氨酸螯合锌组母羊总增重和平均日增重显著增加（P＜0.05)；2）与对照组相比，蛋氨酸螯合锌组和甘氨酸螯合锌组羊奶体细胞数量、乳脂肪、乳蛋白、乳糖、尿素氮、去脂干物质含量差异不显著（P＞0.05），但蛋氨酸螯合锌组羊奶总干物质含量显著提高（P＜0.05)；3）与对照组相比，蛋氨酸螯合锌组和甘氨酸螯合锌组羊奶氨基酸含量差异不显著（P＞0.05）；4）与对照组相比，蛋氨酸螯合锌组羊奶正己酸、辛酸、月桂酸、十三酸、γ-亚麻酸含量显著提高（P＜0.05)，甘氨酸螯合锌组羊奶木蜡酸、顺-11-二十烯酸、γ-亚麻酸含量显著提高（P＜0.05)。综上，蛋氨酸螯合锌可增加羊奶中的总干物质含量，甘氨酸螯合锌可降低羊奶中单不饱和脂肪酸、多不饱和脂肪酸含量且都可提高妊娠母羊总增重和平均日增重，说明补饲蛋氨酸螯合锌和甘氨酸螯合锌均可提高妊娠母羊的生长性能并改变羊奶品质。
Subjects: Biology >> Zoology submitted time 2018-12-24 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究饲粮2-羟基-4-(甲硫基)丁酸异丙酯（HMBi）添加水平对山羊生长性能、营养物质表观消化率及血清生化指标和激素水平的影响，以确定山羊饲粮中HMBi的最佳添加水平。选择36只2.5月龄左右、平均体重为（13.33±1.71） kg的山羊，随机分为4组，每组3个重复，每个重复3只（公阉羊1只，母羊2只），各组山羊分别饲喂HMBi添加水平为0（对照组）、0.05%、0.10%、0.20%（干物质基础）的试验饲粮。预试期10 d，正试期40 d。结果显示：1）0.10%组山羊的平均日增重最高，为158.89 g/d，料重比最低，为4.82，经济效益最佳，且其平均日增重和毛收益均显著高于对照组（P＜0.05），其余各组的平均日增重分别为：0.20%组，141.11 g/d；0.05%组，140.56 g/d；对照组，119.44 g/d。2）干物质、中性洗涤纤维、酸性洗涤纤维和消化能表观消化率各组间均无显著差异（P＞0.05）。0.10%组的粗蛋白质表观消化率显著高于对照组（P＜0.05）。3）试验第1天，各组山羊血清尿素氮（UN）、葡萄糖（GLU）、甘油三酯（TG）、总胆固醇（TC）、游离脂肪酸（NEFA）、总氨基酸（TAA）含量及生长激素（GH）与类胰岛素生长因子-Ⅰ（IGF-Ⅰ）水平均无显著差异（P>0.05）。试验第31天，0.20%组山羊血清TG含量显著高于其他3组（P0.05）。由此说明，饲粮中添加HMBi有利于提高山羊对蛋白质的消化利用，促进山羊生长，且添加水平为0.10%（干物质基础）时经济效益最佳。
Subjects: Biology >> Zoology submitted time 2017-10-10 Cooperative journals: 《动物营养学报》
Abstract：反刍动物能够将人类不能利用的粗饲料转化为优质蛋白质—肉和奶，这对农业以及人类社会有着重要意义。但是这种转化效率与植物细胞壁的消化率有重要关系，目前植物性原料消化率较低，影响了转化效率。在反刍饲粮中补充外源酶制剂具有提高植物纤维消化的潜力，从而提高饲粮的利用效率。本文总结了外源酶制剂应用现状、作用模式以及发展方向，认为目前外源酶制剂在反刍动物应用上有积极的结果，也存在效果不明显的报道，这可能与酶谱、添加水平、添加方式、底物类型以及宿主的生产水平等方面有关。同时阐述了外源酶制剂是在饲喂前、瘤胃中、瘤胃后的不同环境中起到作用，但详细机制还需要继续深入探究。综上所述，尽管酶制剂应用还存在一些问题，以提高饲料消化率为目标的酶制剂仍将会是反刍动物添加剂发展的一个重要方向。
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