Abstract:
Liquidambar formosana is one of the important native tree species in Guangxi, which has high timber,
ornamental and medicinal value. This study designs and develops EST-SSR markers of L. formosana based on
data from the transcriptome sequencing. The purpose is to provide an effective and reliable molecular marker tool
for studies of population genetics of this species, it is of great significance to the protection and utilization of all
kinds of material resources of the tree. Simple sequence repeats were selected from the transcriptome data in L.
formosana, and then primers were developed, and screened out by PCR amplification and polyacrylamide gel
electrophoresis for high polymorphism, and the efficiency was tested by using 30 individuals from of a wild L.
formosana population. The results were as follows: (1) A total 23 777 SSRs were obtained by searching SSR
unigenes from transcriptome data. The repeat type of the SSR loci in L. formosana was dominated by
mononucleotide repeats (46.54%). The highest proportion of SSR loci (72.36%) was between 5 and 12 times. (2)
A total of 262 pairs of SSR primers were developed, and among them, 139 pairs amplified the target bands with a success rate of 53.1%, and 18 pairs of them that can be used to steadily obtain clear bands were finally identified.
(3) The polymorphism detection showed that all sites had a high degree of polymorphism. The number of alleles,
effective alleles, Shannon diversity index, observed heterozygosity and fixed index of the L. formosana
populations ranged in 2-4, 1.112 8-2.609 6, 0.208 9-1.112 7 and 0.275 9-1.000 0, the average values were 2.333
3, 1.957 4, 0.708 5 and 0.722 6, respectively. In conclusion, the dominant SSR repeat type and repeat motif in L.
formosama are basically the same as those in other species, the developed EST-SSR markers can meet the needs
of population genetic studies of L. formosana, which provides abundant primers for the study of genetic diversity
of L. formosana.
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