摘要: Autophagosome formation is promoted by the PI3 kinase complex and negatively regulated by myotubularin phosphatases, indicating that regulation of local phosphatidylinositol 3-phosphate (PtdIns3P) levels is important for this early phase of autophagy. Here, we show that the Caenorhabditis elegans myotubularin phosphatase MTM-3 catalyzes PtdIns3P turnover late in autophagy. MTM-3 acts downstream of the ATG-2/EPG-6 complex and upstream of EPG-5 to promote autophagosome maturation into autolysosomes. MTM-3 is recruited to autophagosomes by PtdIns3P, and loss of MTM-3 causes increased autophagic association of ATG-18 in a PtdIns3P-dependent manner. Our data reveal critical roles of PtdIns3P turnover in autophagosome maturation and/or autolysosome formation.
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期刊:
EMBO REPORTS
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分类:
生物学
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生物物理学
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生物物理、生物化学与分子生物学
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引用:
ChinaXiv:201605.01803
(或此版本
ChinaXiv:201605.01803V1)
DOI:10.12074/201605.01803V1
CSTR:32003.36.ChinaXiv.201605.01803.V1
- 推荐引用方式:
Wu, Yanwei,Wu, Yanwei,Cheng, Shiya,Zou, Wei,Wang, Xiaochen,Zhao, Hongyu,Zhang, Hong,Yoshina, Sawako,Mitani, Shohei,Yoshina, Sawako,Mitani, Shohei.(2016).PI3P phosphatase activity is required for autophagosome maturation and autolysosome formation.中国生物工程预印本出版平台.[ChinaXiv:201605.01803]
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