摘要: The traditional herb Plumula Nelumbinis is widely used in the world because it has many biological activities, such as anti-inflammation, antioxidant, antihypertension, and butyrylcholinesterase inhibition. However, the action of Plumula Nelumbinis on airway smooth muscle (ASM) relaxation has not been investigated. A chloroform extract of Plumula Nelumbinis (CEPN) was prepared, which completely inhibited precontraction induced by high K+ in a concentration-dependent manner in mouse tracheal rings, but it had no effect on resting tension. CEPN also blocked voltage-dependent L-type Ca2+ channel-(VDCC-) mediated currents. In addition, ACh-induced precontraction was also completely blocked by CEPN and partially inhibited by nifedipine or pyrazole 3. Besides, CEPN partially reduced ACh-activated nonselective cation channel (NSCC) currents. Taken together, our data demonstrate that CEPN blocked VDCC and NSCC to inhibit Ca2+ influx, resulting in relaxation of precontracted ASM. This finding indicates that CEPN would be a candidate of new potent bronchodilators.
-
期刊:
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
-
分类:
生物学
>>
生物物理学
>>
整合生物学
-
引用:
ChinaXiv:201605.01496
(或此版本
ChinaXiv:201605.01496V1)
DOI:10.12074/201605.01496V1
CSTR:32003.36.ChinaXiv.201605.01496.V1
- 推荐引用方式:
Tan, Li,Chen, Weiwei,Wei, Ming-Yu,Shen, Jinhua,Yu, Meng-Fei,Liu, Qing-Hua,Tan, Li,Chen, Weiwei,Wei, Ming-Yu,Shen, Jinhua,Yu, Meng-Fei,Liu, Qing-Hua,Yang, Guangzhong,Guo, Donglin,Guo, Donglin,Qin, Gangjian,Ji, Guangju.(2016).Relaxant Action of Plumula Nelumbinis Extract on Mouse Airway Smooth Muscle.中国生物工程预印本出版平台.[ChinaXiv:201605.01496]
(点此复制)