摘要: Lysine formylation is a newly discovered post-translational modification (PTM) in histones and other nuclear proteins; it has a well-recognized but poorly defined role in chromatin conformation modulation and gene expression. To date, there is no general method to site-specifically incorporate N-epsilon-formyllysine at a defined site of a protein. Here we report the highly efficient genetic incorporation of the unnatural amino acid N-epsilon-formyllysine into proteins produced in Escherichia coli and mammalian cells, by using an orthogonal N-epsilon-formyllysine tRNAsynthetase/tRNA(CUA) pair. This technique can be applied to study the role of lysine formylation in epigenetic regulation.
-
期刊:
CHEMBIOCHEM
-
分类:
生物学
>>
生物物理学
>>
生物物理、生物化学与分子生物学
-
引用:
ChinaXiv:201605.01346
(或此版本
ChinaXiv:201605.01346V1)
DOI:10.12074/201605.01346V1
CSTR:32003.36.ChinaXiv.201605.01346.V1
- 推荐引用方式:
Wang, Tianyuan,Yin, Xuebin,Zhou, Qing,Li, Fahui,Wang, Jiangyun,Yu, Yang,Yin, Xuebin.(2016).Genetic Incorporation of N-epsilon-Formyllysine, a New Histone Post-translational Modification.中国生物工程预印本出版平台.[ChinaXiv:201605.01346]
(点此复制)