摘要: Integrin alpha v beta 6 is widely upregulated in variant malignant cancers but is undetectable in normal organs, making it a promising target for cancer diagnostic imaging and therapy. Using streptavidin-biotin chemistry, we synthesized an integrin alpha v beta 6-targeted near-infrared phthalocyanine dye-labeled agent, termed Dye-SA-B-HK, and investigated whether it could be used for cancer imaging, optical imaging-guided surgery, and phototherapy in pancreatic cancer mouse models. Dye-SA-B-HK specifically bound to integrin alpha v beta 6 in vitro and in vivo with high receptor binding affinity. Using small-animal optical imaging, we detected subcutaneous and orthotopic BxPC-3 human pancreatic cancer xenografts in vivo. Upon optical image-guidance, the orthotopically growing pancreatic cancer lesions could be successfully removed by surgery. Using light irradiation, Dye-SA-B-HK manifested remarkable antitumor effects both in vitro and in vivo. F-18-FDG positron emission tomography (PET) imaging and ex vivo fluorescence staining validated the observed decrease in proliferation of treated tumors by Dye-DA-B-HK phototherapy. Tissue microarray results revealed overexpression of integrin alpha v beta 6 in over 95% cases of human pancreatic cancer, indicating that theranostic application of Dye-DA-B-HK has clear translational potential. Overall, the results of this study demonstrated that integrin alpha v beta 6-specific Dye-SA-B-HK is a promising theranostic agent for the management of pancreatic cancer. (C) 2015 Elsevier Ltd. All rights reserved.
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期刊:
BIOMATERIALS
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分类:
生物学
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生物物理学
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影像医学与生物医学工程
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引用:
ChinaXiv:201605.01329
(或此版本
ChinaXiv:201605.01329V1)
DOI:10.12074/201605.01329V1
CSTR:32003.36.ChinaXiv.201605.01329.V1
- 推荐引用方式:
Gao, Duo,Gao, Liquan,Zhang, Chenran,Liu, Hao,Jia, Bing,Wang, Fan,Liu, Zhaofei,Gao, Duo,Gao, Liquan,Zhang, Chenran,Liu, Hao,Jia, Bing,Wang, Fan,Liu, Zhaofei,Zhu, Zhaohui,Wang, Fan.(2016).A near-infrared phthalocyanine dye-labeled agent for integrin alpha v beta 6-targeted theranostics of pancreatic cancer.中国生物工程预印本出版平台.[ChinaXiv:201605.01329]
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