摘要: Hemocyanins present in invertebrate hemolymph are multifunctional proteins, responsible for oxygen transport and contributing to innate immunity through phenoloxidase-like activity. In arthropods, hemocyanin has been identified as a source of broad-spectrum antimicrobial peptides during infection. Conversely, no hemocyanin-derived antimicrobial peptides have been reported for molluscs. The present study describes a putative antimicrobial region, termed haliotisin, located within the linking sequence between the alpha-helical domain and beta-sheet domain of abalone (Haliotis tuberculata) hemocyanin functional unit E. A series of synthetic peptides based on overlapping fragments of the haliotisin region were tested for their bactericidal potential. Incubating Gram-positive and Gram-negative bacteria in the presence of certain haliotisin peptides, notably peptides 3-4-5 (DTFDYKKFGYRYDSLELEGRS ISRIDELIQQRQEKDRTFAGFLLKGFGTSAS) led to reductions in microbial growth. Furthermore, transmission electron micrographs of haliotisin-treated bacteria revealed damages to the microbial cell wall. Data discussed here provides the first evidence to suggest that molluscan hemocyanin may act as a source of anti-infective peptides. (C) 2014 Elsevier Ltd. All rights reserved.
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期刊:
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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分类:
生物学
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生物物理学
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免疫学
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引用:
ChinaXiv:201605.01299
(或此版本
ChinaXiv:201605.01299V1)
DOI:10.12074/201605.01299V1
CSTR:32003.36.ChinaXiv.201605.01299.V1
- 推荐引用方式:
Zhuang, Jun,Wu, Zujian,Xie, Lianhui,Zhuang, Jun,Wu, Zujian,Xie, Lianhui,Coates, Christopher J.,Zhu, Hongtao,Zhu, Ping,Zhu, Hongtao.(2016).Identification of candidate antimicrobial peptides derived from abalone hemocyanin.中国生物工程预印本出版平台.[ChinaXiv:201605.01299]
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