Subjects: Biology >> Botany >> Applied botany submitted time 2019-08-26 Cooperative journals: 《广西植物》
Abstract： Tapping-enhanced rubber biosynthesis is closely related to the activation of jasmonic acid signaling in laticifer cells of rubber tree. The expression level of genes related to jasmonic acid signaling pathway and rubber biosynthesis were both positively correlated with the dry rubber yield. However, the exact relationship between the expression of gengs involved in jasmonic acid signaling and rubber biosynthesis is yet to be elucidated. In the present study, qPCR was used to analyze the expression of 9 jasmonic acid signaling genes, HbCOI1, HbJAZ1, HbJAZ2, HbJAZ3, HbMYC1, HbMYC2, HbMYC3, HbMYC4, HbMYC5 and 6 rubber biosynthesis genes, HbHRT2, HbSRPP, HbREF, HbHMGR1, HbHRT1, HbGAPDH, in laticifer cells of 5 Wichham germplasms and 5 1981’IRRDB germplasms following tapping them with S/2D d/3 tapping system, Hb18S was used as internal reference gene. The correlation between these 15 genes was analyzed by Pearson correlation coefficient. The bivariate correlation coefficient (r12) and partial correlation coefficient (r12.3) of 105 gene pairs were obtained with the mean ± standard deviation were 0.486 ± 0.220 and 0.304 ± 0.211, respectively. Among them, 63 gene pairs (60%) with r12 in the same direction as r12.3 and 42 genes pairs (40%) in the opposite direction; In the degree of correlation, 23 gene pairs (21.905%) with | r12 | less than | r12.3 |, and 82 gene pairs (78.095%) with | r12 | greater than | r12.3 |. There 76 gene pairs (72.38%) bivariate correlation coefficient (r12) were significant correlation at P＜0.05 level and 59 gene pairs (56.19%) at P＜0.01 level. In contrast, there less partial correlation coefficient (r12.3) were significant correlation at P＜0.05 level (21pairs, 20%) and P＜0.01 level (16 pairs, 15.24%). These results suggest that the expression of genes involved in these two pathways were related to each other. And provides a further evidence for the assumption that “expression correlation of functional related genes”, which is widely adopted in gene expression profile analysis. It can be used for excavating, screening and predicting unknown genes related to rubber biosynthesis, as well as provides a theoretical basis for studying the molecular regulation mechanism of rubber yield formation in Hevea brasiliensis Muell. Arg.
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