Subjects: Biology >> Botany >> Applied botany submitted time 2024-04-17 Cooperative journals: 《广西植物》
Abstract： Expansin (EXP) plays an important role in plant response to environmental stress by regulating cell wall relaxation. To explore the role of EXP genes in soybean response to abiotic stress, two soybean EXP genes (GmEXPB5 and GmEXPB7) and their protein sequences were analyzed by bioinformatics, and their expression levels were detected by real-time fluorescent quantitative PCR (qRT-PCR). The results were as follows: (1) The GmEXPB5 and GmEXPB7 were located on chromosomes 10 and 12 of soybean, and encoded proteins containing 272 and 267 amino acids, respectively. The molecular weight of GmEXPB5 protein was 29.07 kD and the theoretical isoelectric point was 7.51. The molecular weight of GmEXPB7 protein was 29.09 kD and the theoretical isoelectric point was 8.66. Both GmEXPB5 and GmEXPB7 were stable hydrophilic proteins localized in the cell wall. Both GmEXPB5 and GmEXPB7 proteins contained a signal peptide sequence and a conserved DPBB_1 domain. (2) GmEXPB5 was closely related to CaEXPB15 of chickpea, and GmEXPB7 was closely related to EXPB3 of chickpea, red bean and cowpea. (3) GmEXPB5 and GmEXPB7 were expressed in soybean roots, stems and leaves, and their expression levels in roots and leaves were significantly higher than those in stems. (4) GmEXPB5 and GmEXPB7 could respond to salt, drought and cold stresses in soybean seedlings. (5) The promoter region of GmEXPB5 contained two types of stress-related cis-elements (ABRE and ARE). The promoter region of GmEXPB7 contained five types of stress-related cis-elements (ABRE、ARE、CGTCA-motif、TC-rich repeats and MBS). These results indicated that GmEXPB5 and GmEXPB7 could participate in the response of soybean to abiotic stress.
Subjects: Biology >> Zoology submitted time 2018-12-25 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究围产期母猪饲粮中添加壳寡糖（COS）对母猪、仔猪免疫功能和母猪肠道微生物的影响。选用体重、背膘厚度、胎次和预产期接近的40头健康大约克妊娠母猪，随机分为2个组，每组20个重复，每个重复1头母猪。对照组饲喂基础饲粮，试验组饲喂在基础饲粮中添加30 mg/kg COS的试验饲粮。试验期为母猪妊娠第90天至分娩后第7天。结果显示：1）围产期母猪饲粮中添加COS对母猪产程、总产仔数、产活仔数、仔猪初生窝重、仔猪平均初生重均无显著影响(P＞0.05)。2）围产期母猪饲粮中添加COS显著提高了母猪血清中免疫球蛋白G(IgG)、免疫球蛋白A(IgA)和白细胞介素-6（IL-6）含量（P＜0.05），对血清中免疫球蛋白M(IgM)、白细胞介素-2（IL-2）和肿瘤坏死因子-α（TNF-α）含量无显著影响(P＞0.05)。3）围产期母猪饲粮中添加COS显著提高了母猪初乳中IgG、IL-2和IL-6含量（P＜0.05），对IgA、IgM和TNF-α含量无显著影响(P＞0.05)。4）围产期母猪饲粮中添加COS显著提高了初生仔猪血清中IL-2和IL-6含量（P＜0.05），并显著降低血清中IgA的含量（P＜0.05）。5）围产期母猪饲粮中添加COS显著降低了母猪粪便中沙门氏菌的数量（P＜0.05），并有降低粪便中大肠杆菌数量的趋势（P＜0.10）。由此可见，围产期母猪饲粮中添加COS能够改善母猪肠道健康，一定程度上提高产活仔数，显著增强母猪及初生仔猪的免疫功能。
Subjects: Biology >> Zoology submitted time 2017-10-23 Cooperative journals: 《动物营养学报》
Abstract：本文旨在研究颈静脉灌注精氨酸对泌乳中期奶牛血清生化和免疫指标的影响。采用3×3复式拉丁方试验设计，选择6头体重、胎次、泌乳期、泌乳量和体况基本一致的荷斯坦奶牛为试验动物，随机分为3组（每组2头），分别为酪蛋白组（对照组）、精氨酸组[灌注37.66 g/(L·d)]、丙氨酸组[与精氨酸组等氮，灌注77.24 g/(L·d)]；每个试验期为22 d（7 d灌注+15 d间隔），在每个试验灌注期的最后1 d晨饲前采血，测定血清生化和免疫指标。结果表明：精氨酸组的奶牛血清中总蛋白、免疫球蛋白G(IgG)、肿瘤坏死因子α（TNF-α）浓度要显著高于其他2组（P0.05）。综上，灌注精氨酸能够通过提高血清总蛋白、IgG及IL-1、TNF-α的浓度，从而一定程度上增强泌乳奶牛机体的免疫力和胎儿的被动免疫力。
Subjects: Biology >> Zoology submitted time 2017-10-10 Cooperative journals: 《动物营养学报》
Abstract：本文旨在研究颈静脉灌注精氨酸对泌乳中期奶牛泌乳性能和牛乳酪蛋白合成的影响。将6头体重、胎次、泌乳期、泌乳量和体况基本一致的荷斯坦奶牛随机分为3组（每组2头）：酪蛋白模式组（对照组）、精氨酸灌注组、丙氨酸等氮组（与精氨酸灌注组等氮），采用3×3复拉丁方试验设计，每期22 d（7 d灌注期+15 d间隔期），测定其泌乳性能、酪蛋白含量以及酪蛋白基因的表达情况。结果表明：1）灌注的第5天精氨酸灌注组的乳蛋白及乳中非脂固形物含量均显著高于酪蛋白模式组（P0.05）；κ-酪蛋白含量则以精氨酸灌注组为最高，显著高于其他2组（P<0.05）。3）精氨酸灌注组αs1-酪蛋白基因（CSN1S1）、αs2-酪蛋白基因（CSN1S2）的表达量显著高于其他2组（P<0.05）。综上，灌注精氨酸提高了乳蛋白中α-酪蛋白和κ-酪蛋白含量，以及CSN1S1、CSN1S2在奶牛乳腺组织的表达量，有利于牛乳中乳蛋白率和乳品质的提高。
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