Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》
Abstract： The secondary laticifer is the position for synthesis and storage of natural rubber (NR), which is differentiated from the vascular cambium of bark in stem of rubber trees (Hevea brasiliensis). The quantity of secondary laticifer is depend on the frequency of the secondary laticifer differentiation from cambia, which is the main index of yield breeding of rubber tree. In previous studies, we found trichostatin A (TSA), an inhibitor of histone deacetylase (HDA), can also induce laticifer differentiation, and the histone deacetylase gene (HbHDA6) is a participator in laticifer differentiation. Because of the molecular mechanism of secondary laticifer differentiation regulated by histone acetylation has not been clarified. So, we construct a yeast two-hybrid cDNA library used the vascular cambium tissues treatment by coronatine (COR), and screening the yeast two-hybrid library by HbHDA6 gene as the bait, for determining the proteins interacting with HbHDA6. The results were as follows: (1) The homogenized yeast two-hybrid cDNA library of vascular cambium is successful and constructed by the technology of gateway. The capacity of the primary library is 6.3 × 106, the total number of clones is 1.2 × 107, and the capacity of secondary library is 7.7 × 106, the total number of clones is 1.5 × 107, the recombination rate of the libraries is 100%. (2) The average length of inserted fragments is 1.1 kb and 1.2 kb in primary and secondary library, respectively. The bait vector of pGBKT7-HbHDA6 is constructed and confirmed no self-activation activity. (3) The cDNA library is screened with the pGBKT7-HbHDA6 bait vector, found 22 proteins interacting with HbHDA6, including CLP1, ERF3, ERF4, HSP82, LARP6a, APT5, PP2A, APT5, FBA6, etc. The results provide a theoretical basis for analyzing the molecular regulatory network of the secondary laticifer differentiation of rubber tree, and provide candidate genes for the rubber production potential of genetically modified and a new clue for the genetic improvement and breeding of high-performance NR.
Subjects: Biology >> Botany >> Applied botany submitted time 2020-03-24 Cooperative journals: 《广西植物》
Abstract：以药食两用植物绞股蓝为材料，研究了植物激素乙烯对绞股蓝皂苷生物合成关键酶基因表达及皂苷含量的影响。实验采用荧光定量PCR技术，检测了乙烯利处理后绞股蓝不同器官中皂苷生物合成关键酶基因GpFPS，GpSS和GpSE的表达水平；采用分光光度法及HPLC技术，测定了乙烯利处理对绞股蓝总皂苷和皂苷单体Rb1、Rb3和Rd含量的影响。结果表明：外施乙烯利能够不同程度地上调GpFPS，GpSS和GpSE基因的表达水平，且3个基因的表达模式在不同器官间不同，而在同一器官中相似。在乙烯利处理后3 d，所测各器官中的总皂苷含量与对照相比均有所上升，其中根、成熟叶和幼叶达到显著水平，但3个皂苷单体在不同器官中的增加或降低并不一致，以Rb3含量最高。该结果为探索利用植物激素调控绞股蓝皂苷次生代谢提供了参考。
Subjects: Biology >> Botany >> Applied botany submitted time 2019-08-26 Cooperative journals: 《广西植物》
Abstract： Protein is one of the basic components of life system and the executor of most biological functions. Protein abundance is closely related to its biological function, and its abundance is strictly and precisely regulated by each link in the process of gene expression. Among them, it is known that, there is a strong correlation between protein abundance and its corresponding mRNA abundance, about 40% of the difference in protein abundance can be explained by mRNA abundance. Jasmonic acid signaling pathway regulates the biosynthesis of natural rubber in Hevea brasiliensis, but the difference of expression abundance among related genes is yet to be elucidated. In the present study, the expression abundance differences of 15 rubber biosynthesis regulatory genes HbCOI1, HbJAZ1, HbJAZ2, HbJAZ3, HbMYC1, HbMYC2, HbMYC3, HbMYC4, HbMYC5, HbGAPDH, HbHMGR1, HbSRPP, HbREF, HbHRT1, HbHRT2, and 2 common internal reference genes Hb18S, HbACTIN1 in 10 rubber tree germplasms latex following tapping them with S/2D d/3 tapping system were compared. The expression abundance of HbACTIN1 in each sample is set to 1, and the expression abundance of other genes in the sample is calculated according to the standard. The results showed that the transcriptional abundance of different genes in the same individual was significantly different, and the abundance order of the same gene set was different in different individuals. The transcription abundance of the same gene was significantly different in different individuals, the maximum abundance of the 16 genes were 9.43, 6.04, 10.02, 12.29, 18.82, 9.22, 38.46, 112.83, 121.36, 15.34, 19.09, 13.54, 10.05, 19.80, 24.83, 11.82 times of the lowest abundance, respectively. Correspondingly, the coefficient of variation were 73.05, 55.19, 69.09, 67.37, 66.59, 53.87, 83.25, 122.02, 166.34, 59.89, 70.59, 75.67, 74.20, 68.34, 84.23, 78.59 percent. Overall, at the population level, the transcription abundance of the 16 genes from high to low were 18S＞SRPP＞HMGR1＞REF＞MYC2/HRT1＞COI1＞MYC1/MYC4＞GAPDH/JAZ1/MYC5＞JAZ2＞HRT2/MYC3/JAZ3, correspondingly, the average abundance were 28382.26, 43.64, 11.39, 7.16, 5.47, 5.10, 1.07, 0.75, 0.74, 0.45, 0.42, 0.33, 0.12, 0.06, 0.06, 0.04 times than that of HbACTIN1 respectively. It is worth noting that, the abundance of 18s is undoubtedly the highest; and in mRNA, SRPP is the largest, JAZ1 is greater than that of JAZ2 and JAZ3, MYC2 is greater than that of MYC1, MYC3, MYC4 and MYC5, HRT1 is greater than HRT2 at both the individual and population levels. The results showed that, the abundance of structural genes and functional genes is higher than that of regulatory genes. In the analysis of gene relative expression, the target gene and the internal reference gene are usually homogenized, thus masking the real abundance difference between different genes, therefore, in the gene expression analysis, we should pay attention not only to the relative expression of genes, but also to the abundance difference between genes, which is helpful to understanding the function of genes in a more comprehensive way.
Subjects: Biology >> Botany >> Applied botany submitted time 2018-12-19 Cooperative journals: 《广西植物》
Abstract：割胶促进橡胶树合成天然橡胶与激活乳管细胞的茉莉酸信号途径密切相关。茉莉酸信号途径关键环节的基因表达水平与干胶产量的相关性尚不清楚。本文采用qPCR技术，分析了割胶条件下茉莉酸信号途径关键环节的9个相关基因在5个橡胶树魏克汉种质和5个1981'IRRDB种质乳管细胞中的表达。结果表明，绝大多数魏克汉种质的株次干胶量显著高于1981'IRRDB种质。在9个基因中，除了HbMYC4和HbMYCS，其余7个基因在绝大多数橡胶树魏克汉种质中的表达量均显著高于1981'IRRDB种质，尤其是HbMYC3基因表达的差异性好，与干胶产量相关性高，有望作为橡胶树产量育种的一个的分子标记。
Hits
Hits
Downloads
Comment