Subjects: Biology >> Botany >> Applied botany submitted time 2020-08-02 Cooperative journals: 《广西植物》
Abstract：为了解高山杜鹃的光合能力在不同季节对环境因子适应的动态变化，以被广泛引种栽培的马缨杜鹃（Rhododendron delavayi）为材料，露天种植在2 加仑塑料盆中，利用气体交换技术和叶绿素荧光技术测定了不同生长时期马缨杜鹃光合特性的动态变化，探讨不同生长阶段影响马缨杜鹃光合作用的主要生理生态因子。结果表明：在云南昆明地区，马缨杜鹃的生长与其光合变化重叠，光合特性的季节变化可准确反映其生长周期的变化。在5 月—7 月的生长初期，马缨杜鹃的光合速率（Pn）快速增加，气孔导度（Gs）是此阶段影响马缨杜鹃光合速率的决定性因素，空气相对湿度（RH）则通过影响Gs 和蒸腾速率（Tr）来对Pn 产生较大的间接效应，可通过增加RH 来激发光合潜能以促进生长；在7 月—10 月的生长盛期，RH高，Gs 大，Pn 较高且稳定，但受昆明地区独特气候的影响，该时期内较低的气温（Temp）是影响该时期光合生理的重要因子，此阶段可通过增加Temp 来提高Pn 以促进马缨杜鹃的快速生长；在10 月之后的生长后期，Temp 和RH 快速下降，气孔关闭，光合系统Ⅱ的光能转化效率（Fv/Fm）和Pn 逐渐降低，Gs 和Fv/Fm是该阶段影响光合作用的主要因子。研究结果可为马缨杜鹃的科学栽培及推广应用提供重要参考。
Subjects: Biology >> Botany >> Applied botany submitted time 2020-08-02 Cooperative journals: 《广西植物》
Abstract：为解决木本切花植物帝萝花‘璀璨明珠’繁殖效率低的问题，以其幼嫩枝芽为外植体，研究不同基本培养基对长势的影响、不同激素种类和浓度对增殖和生根的效果，揭示了‘璀璨明珠’离体繁殖的生长特点，建立了高效的帝萝花‘璀璨明珠’组培快繁技术体系。结果表明：‘璀璨明珠’幼嫩枝芽消毒的方法是0.1%的升汞溶液浸泡12 min，污染率为21.5 %；外植体在WPM+ZT1 mg﹒L-1+NAA0.1 mg﹒L-1培养基上，侧芽萌发率为73%。增殖的最佳培养基为MS + BA0.4 mg﹒L-1 + NAA0.05 mg﹒L-1，增殖系数为6.63，增殖方式为侧芽增殖和植株基部丛生芽增殖; 生根的适宜培养基为MS + IBA 0.75 mg﹒L-1+ NAA 1 mg﹒L-1，生根率70%。生根瓶苗移栽于珍珠岩和细草炭体积比0.5﹕1的基质中，光照强度10 000～12 000 lx，空气湿度70%～80%下培养，60 d 后成活率可达72%。研究结果为帝萝花组培种苗的商业化生产提供了技术支撑，促进了该高档木本切花的推广、种植和产业化。
Subjects: Biology >> Botany >> Applied botany submitted time 2018-10-26 Cooperative journals: 《广西植物》
Abstract： Rhododendron is a world famous ornamental garden plant and has significant economic and ornamental value. Light has an important impact on the growth and development of Rhododendron. However, the studies on the demand and adaptability of Rhododendron are scarce. In order to understand the demand and adaptability of Rhododendron to light, 3-year-old plants of Rhododendron ‘Furnivall′s Daughter’ were used as materials and the effects of shading on leaf anatomical structure and photosynthesis were studied. The results showed that: the impact of light intensity on the stomata density of Rhododendron ‘Furnivall′s Daughter’ is not obvious, and the stomatal density ranged from 299.70 to 327.22 mm-2 . However, the stomatal size and the area of stomata apparatus were significantly influenced by light intensity. The plants of 100% and 30% full light intensity had the minimum and maximum stomatal size, respectively. As the light intensity decreased, the thickness of leaf, palisade tissue, sponge tissue, as well as the thickness of adaxial epidermis and abaxial epidermis decreased to improve the light use efficiency of leaves. The plasticity analysis showed that the plasticity index of mesophyll-related parameters such as leaf thickness, abaxial epidermis thickness, and the thickness of palisade and spongeb tissue were higher, while that of stomatal-related parameters such as stomatal density, stomatal length and width were lower, which indicated that mesophyll tissue plays a more important role in the process of adaption to different light environment. The analysis of light response curves and photosynthetic parameters showed that the plants of 100% full light intensity were inhibited and damaged by strong light, and the plants showed the lowest light saturation point (LSP), net photosynthetic rate (Pn), photosynthetic rate at light saturation point (Pmax), stomatal conductance (Gs) and transpiration rate (Tr). After shading treatments, the Pn, Pmax, Gs, Tr and light use efficiency (LUE) were improved. Compared with other shading treatments, the plants of 30% full light intensity not only had the lowest light compensation point (LCP), dark respiration rate (Rd) but also had the highest LSP, Pn, Pmax, Gs, Tr and LUE. The above results suggest that the optimum light intensity of Rhododendron ‘Furnivall′s Daughter’ in Kunming is about 30% full light intensity. In the cultivation and application of Rhododendron, we should take some shading measures to meet the optimum light conditions for its growth
Subjects: Biology >> Bioengineering submitted time 2018-02-11 Cooperative journals: 《中国生物工程杂志》
Abstract：目的 制备鼠抗树鼩CD3ε单克隆抗体，并对其生物学特性进行鉴定。方法 以GST-CD3ε蛋白为免疫原免疫BALB/c小鼠，利用杂交瘤技术融合免疫后的BALB/c小鼠脾细胞和小鼠骨髓瘤SP2/0细胞，通过间接ELISA方法检测，筛选出多株能分泌抗树鼩CD3ε的杂交瘤细胞株，经过3次亚克隆筛选后，制备小鼠腹水单克隆抗体，并纯化得到鼠抗树鼩CD3ε单克隆抗体，通过腹水单克隆抗体效价测定、单克隆抗体亲和力测定、单克隆抗体抗原表位分析、 Western blot和FACS分析其生物学特性。结果 筛选到5株能稳定分泌抗树鼩CD3ε单克隆抗体的杂交瘤细胞株，命名为78I、87I、92D1、75II和35C8，腹水单克隆抗体效价分别为1:106、1:106、1:104、1:106、1:103，亲和力解离常数（Kd）分别为1.8×10-5、2.9×10-5、4.9×10-5、7.3×10-5、3.6×10-5。5株单克隆抗体抗原表位分析显示78I、87I和75II识别同一抗原表位，而92D1和35C8识别另一抗原表位。经Western blot检测，HRP标记后的92D1能识别GST-CD3ε蛋白和树鼩PBMC，并对大鼠、小鼠和猴的PBMC有抗体交叉反应。经FACS检测，PE-Cy5.5标记后的92D1能特异性识别树鼩PBMC。结论 成功制备出鼠抗树鼩CD3ε的单克隆抗体，为进一步应用于树鼩免疫检测奠定基础。
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