Subjects: Biology >> Botany >> Applied botany submitted time 2020-05-28 Cooperative journals: 《广西植物》
Abstract： With the aim of exploring the application direction and approach of wild lilies from Qin-Ba mountains in breeding and obtain breeding materials with specific genetic background, with the help of cut-style pollination and embryo rescue, 6 species from Qin-Ba mountains, 1 cultivar from Asiatic hybrids (AA), 3 cultivars from Oriental hybrids (OO) and 3 cultivars from OT hybrids (OT) were selected for interspecific hybridization, and 32 crossing combinations, with a total of 263 flowers, were taken, embryo and embryo sac were cultured isolated from expanded seedpots. Results showed that: (1) There was a significant difference between different recombination, the general fruit setting rate was 11.4%, 30 expanded seedpots were taken for embryo rescue, 38 embryo (sac) were rescued and finally 7 plants were obtained. (2) Six hybrid plants were obtained in expanded seedpots of 5 crossing combinations from 12 crossing combinations when crossing between different lily species. (3) In addition to ‘Elite’ × L. pumilum can directly harvest seeds, 1 hybrid plants was obtained in expanded seedpots of 9 crossing combinations from 20 crossing combinations when crossing cultivars as mothers and species as fathers. (4) Lilium Regale and L. leucanthum were successfully used in interspecific hybridization of TT ×AA and backcross of OO ×TT, and hybrid plants were obtained. (5) There was a significant difference between lily species when using as crossing materials, more hybrids were got when crossing using species from Trumpet group (section Leucolirion) and no hybrid were obtained when crossing using species from section Archelirion and Sinomartagon. But L. Brownii did not get offspring. Results revealed that it was easier to obtain crossing hybrids when crossing was made between different lily species, TT ×AA crossing using L. regale, L. leucanthum as father with Asiatic cultivars, TT ×AA crossing using L.duchartrei as father, and OT×TT crossing using OT cultivars as mother and L. regale, L. leucanthum as father were new ways to breed new lily cultivars; the way of using L. brownii still need to be explored.
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