Subjects: Biology >> Biochemistry submitted time 2024-05-25
Abstract： Taking compound nutrient supplements is a common means of health management. No studies have investigated the overall effects of complex nutrient supplements on healthy individuals from the perspective of the urine proteome. In this study, we compared and analyzed the urine proteome of healthy individuals after taking compound nutrient supplements for 2 weeks, 4 weeks and before taking them, and found that the urine proteome changed more significantly after taking compound nutrient supplements for 2 weeks, and the differential proteins and their enriched pathways may be associated with nutrient supplementation, such as erythropoietin receptor (fold change of 449.5 after taking compound nutrient supplements for 2 weeks). The results of this study provide new clues about the health effects of complex nutrient supplements from the perspective of the urine proteome and help to optimize guidelines and recommendations for the use of complex nutrient supplements.
Peer Review Status:
Awaiting Review
Subjects: Biology >> Botany >> Applied botany submitted time 2024-04-17 Cooperative journals: 《广西植物》
Abstract： To explore the effect of exogenous polyamines (PAs) on embryogenic callus (EC) proliferation and somatic embryogenesis of litchi, the morphology, structure, endogenous PA content and related enzyme activities were systematically investigated using the ‘Feizixiao’ ECs as materials subcultured on the medium supplemented with various PAs. The results were as follows: (1) The exogenous putrescine (Put), spermidine (Spd), and spermine (Spm) treatment significantly increased the EC proliferation rate and reduced the amount of induced somatic embryos and regenerated plantlets. The proliferated embryogenic cells after exogenous PA treatments were more consistent in size and stained deeply and evenly. Furthermore, multicellular proembryos in EC were reduced, and fully differentiated early cotyledon embryos could be seen. (2) All the exogenous PA treatments significantly increased the endogenous PA content in EC. Among them, Put treatment had the highest content of each endogenous PA component and total PA. When the EC proliferated on the medium containing exogenous PAs was transferred to the medium without exogenous PAs (M3) for proliferating, the Put content in the EC was still significantly higher than the control, however, the endogenous Spd and Spm were significantly decreased. (3) Exogenous Put treatment significantly increased the activities of ornithine decarboxylase (ODC), arginine decarboxylase (ADC), and diamine oxidase (DAO) in EC, while exogenous Spd and Spm treatments significantly reduced the activities of ADC and DAO in EC, and exogenous Spd significantly increased PAO activity. When transferred to the M3 medium, the ADC and DAO activities of newly proliferated EC were significantly lower than those of EC cultured with exogenous PAs, but there was no significant difference in ODC and PAO activities. In summary, the exogenous PAs can affect endogenous PA content by regulating the activity of enzymes related to polyamine metabolism, thereby affecting EC proliferation and somatic embryo induction in litchi. These results would provide a basis for further study on the mechanism of PAs regulating litchi regeneration in vitro.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-04-17 Cooperative journals: 《广西植物》
Abstract： Expansin (EXP) plays an important role in plant response to environmental stress by regulating cell wall relaxation. To explore the role of EXP genes in soybean response to abiotic stress, two soybean EXP genes (GmEXPB5 and GmEXPB7) and their protein sequences were analyzed by bioinformatics, and their expression levels were detected by real-time fluorescent quantitative PCR (qRT-PCR). The results were as follows: (1) The GmEXPB5 and GmEXPB7 were located on chromosomes 10 and 12 of soybean, and encoded proteins containing 272 and 267 amino acids, respectively. The molecular weight of GmEXPB5 protein was 29.07 kD and the theoretical isoelectric point was 7.51. The molecular weight of GmEXPB7 protein was 29.09 kD and the theoretical isoelectric point was 8.66. Both GmEXPB5 and GmEXPB7 were stable hydrophilic proteins localized in the cell wall. Both GmEXPB5 and GmEXPB7 proteins contained a signal peptide sequence and a conserved DPBB_1 domain. (2) GmEXPB5 was closely related to CaEXPB15 of chickpea, and GmEXPB7 was closely related to EXPB3 of chickpea, red bean and cowpea. (3) GmEXPB5 and GmEXPB7 were expressed in soybean roots, stems and leaves, and their expression levels in roots and leaves were significantly higher than those in stems. (4) GmEXPB5 and GmEXPB7 could respond to salt, drought and cold stresses in soybean seedlings. (5) The promoter region of GmEXPB5 contained two types of stress-related cis-elements (ABRE and ARE). The promoter region of GmEXPB7 contained five types of stress-related cis-elements (ABRE、ARE、CGTCA-motif、TC-rich repeats and MBS). These results indicated that GmEXPB5 and GmEXPB7 could participate in the response of soybean to abiotic stress.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-04-17 Cooperative journals: 《广西植物》
Abstract： To investigate and clarify the aroma components and contents of different varieties of Guizhou rabbit-eye blueberries, this study used headspace solid phase microextraction and gas chromatography-mass spectrometry (HS-SPME/GC-MS) methods to analyze the aroma component content of the main rabbit-eye blueberry grown in Guizhou, such as ‘Coastal’, ‘Britewell’, ‘Climax’, ‘Tifblue’ and ‘Powderblue’. The results were as follows: (1) A total of 46 aroma components in 7 categories were detected in the 5 varieties, including 8 types of alcohols, 13 types of terpens, 9 types of benzenes, 3 types of aldehydes, 5 types of esters, 4 types of alkanes, and 4 types of others. (2) ‘Climax’ has the most aroma components with 33, while ‘Powderblue’ has the least with only 24; among the aroma components of each variety, aldehydes account for the largest proportion, followed by benzenes, with ‘Britewell’ having the highest content of aldehydes (59.32%) and ‘Powderblue’ the highest content of benzenes (42.58%). (3) Among the five varieties, ‘Coastal’ has the highest total content of aroma components, at 172 872.20 ng‧g-1, followed by ‘Britewell’ at 162 200.87 ng‧g-1; ‘Coastal’ and ‘Powderblue’ have lower total aroma component contents, at 91 284.45 ng‧g-1 and 97 511.10 ng‧g-1, respectively. The significant differences in aroma components and contents among these five rabbit-eye blueberry varieties provide an important basis for the selection of blueberry varieties and the choice of raw materials.
Subjects: Biology >> Botany >> Applied botany submitted time 2024-05-15 Cooperative journals: 《广西植物》
Abstract： Based on the 25 ha karst forest big plot in Gulinqing, Yunnan, through sample-plot investigation, specimen collection and identification, floristic analysis, Jaccard similarity coefficient and PCA, we explored its floristic characteristics and the status of the Gulinqing big plot in Yunnnan and the geographical relationships among Gulinqing in Yunnan, Bubeng in Yunnan, and Nonggang in Guangxi, China. The results were as follows: (1) There were about 78 families, 238 genera, and 406 species（including infraspecies taxa and excluding woody vines）of woody plants with DBH≥ 1cm. (2) In the plot, the families with two–five species and with one species were dominant and accounted for 37.18% and 34.62% of the total number of families, respectively, while the genera with two–four species and with one species were also dominant and accounted for 30.25% and 65.13% of the total number of genera, respectively. (3) Through analysis of the areal-types of families and genera, it was found that it had been divided into 9 areal-types and 7 variations at the family level, and the proportion of families was the highest at Pantropic, accounting for 50.77%, and the ratio of tropical flora to temperate flora (R/T) was 4.42; there were 11 areal-types at the genera level, mainly composed of Tropical Asian, Pantropic, Old World Tropics, 33.47%, 18.22%, and 13.98% respectively, and with R/T of the genera was 10.25. (4) The composition of Gulinqing, Bubeng and Nonggang big plots showed that Gulinqing and Bubeng had the highest similarity in family and genera level at Jaccard similarity coefficient (0.674 and 0.395, respectively), and Bubeng and Nonggang with the smallest Jaccard similarity coefficient (0.575 and 0.297, respectively). (5) PCA principal component analysis of the floristic spectrum had similar result of the Jaccard similarity coefficient above in the level of genera, but different one at the level of families, e.g. a higher geographical connection between Gulinqing and Nonggang. In summary, this plot has a high diversity of families and genera, significant tropical characteristics, ancient floristic characteristics, obvious transitional status and extensive flora connections. However, there is an inconsistent geographical connection among Gulinqing, Bubeng and Nonggang from the composition and floristic spectrum.
Subjects: Biology >> Biochemistry submitted time 2024-04-24
Abstract： How to differentiate the diagnosis of depression and bipolar disorder has always been an important problem that needs to be solved urgently in clinical practice. In this study, from the perspective of urine proteomics, urine samples of similar age were collected from two hospitals to investigate the candidate biomarkers for differentiating the diagnosis of depression and bipolar disorder using both group analysis and one-to-many analysis. The experimental results of the paired group analysis showed that 108 differential proteins were identified in the depressed group compared to the bipolar group under strict screening conditions with screening criteria of FC ≥ 2 or ≤ 0.5 and a two-tailed unpaired t-test of P < 0.01, with an average of 3.7 randomly generated differential proteins, and a confidence level of 96.6 % for the correlation between these proteins and the disease difference. In the one-to-many analysis, 24 differential proteins were co-identified by the samples of 13 depressed patients, 16 of which showed a completely consistent trend of expression changes in all depressed patients studied, and 6 of which were associated with immunoglobulins; 41 differential proteins were co-identified by the samples of 12 depressed patients out of 13, and 19 of which showed a completely consistent trend of expression change in the These results reflect the strong consistency of differential proteins between the two groups of patients. 12 or more samples from depressed patients were enriched for differential proteins related to multiple biological processes and signaling pathways associated with the immune system, which is consistent with previous studies: immune mechanisms may be one of the pathogenetic mechanisms of major depression and that drugs with major immune targets can improve depressive symptoms. In the future, it may be possible to observe the immune status of patients with depression to provide direction and basis for the precise treatment of depression. The results of this paper show that urine proteomics can differentiate between depression and bipolar disorder, suggest possible mechanisms and potential targets for the treatment of depression and bipolar disorder, and provide a tool for future differential diagnosis and precision treatment of the diseases.
Peer Review Status:Awaiting Review
Subjects: Biology >> Molecular Biology submitted time 2024-05-24
Abstract： Differential and even opposing functions of two major antioxidant transcription factors Nrf1 and Nrf2 (encoded by Nfe2l1 and Nfe2l2, respectively) are determined by distinctions in their tempospatial positioning, topological repartitioning, proteolytic processing, and biochemical modification, as well as in their shared evolutionary origin. As a matter of fact, the allelopathic potentials of Nrf1 and Nrf2 (both resembling two entangled ‘Yin-Yang’ quanta that comply with a dialectic law of the unity of opposites) are fulfilled to coordinately control redox physiological homeostasis so as to be maintained within the presetting thresholds. By putative exponential curves of redox stress and intrinsic anti-redox capability, there is inferable to exist a set point at approaching zero with the ‘Golden Mean’ for the healthy survival (i.e., dubbed the ‘zero theory’). A bulk of the hitherto accumulating evidence demonstrates that the set point of redox homeostasis is dictated selectively by multi-hierarchical threshold settings, in which the living fossil-like Nrf1 acts as a robust indispensable determinon, whereas Nrf2 serves as a versatile chameleon-like master regulon, in governing the redox homeodynamic ranges. This is attributable to the facts that Nrf2 has exerted certain ‘double-edged sword’ effects on life process, whereas Nrf1 executes its essential physiobiological functions, along with unique pathophysiological phenotypes, by integrating its ‘three-in-one’ roles elicited as a specific triplet of direct sensor, transducer and effector within multi-hierarchical stress responsive signaling to redox metabolism and target gene reprogramming. Here, we also critically reviewed redox regulation of physio-pathological functions from the eco-evo-devo perspectives, through those coding rules (redox code, stress-coping code, and topogenetic code). The evolving concepts on stress and redox stress were also further revisited by scientific principles of physics and chemistry, apart from two novel concepts of ‘oncoprotists’ and ‘reverse central dogma’ being introduced in this interdisciplinary and synthetic review.
Peer Review Status:Awaiting Review
Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》
Abstract： In order to adjust the structure of inefficient Pinus massoniana forest and explore the dynamic changes for DOC during the mixed decomposition process of P. massoniana and native broad-leaved tree species. Our research focuses on the leaf litter of P. massoniana, Cinnamomum camphora and Toona sinensi, and combine them into the 15 treatments (3 single tree treatments + 12 mixed treatments) according to different tree species combinations and mass ratios, and then conducted field decomposition experiments to further explore the optimal combination for DOC release. The results showed that: (1) DOC content of Pinus massoniana and most of mixed treatments (except for PT64) significantly increased during the early stages of decomposition (0-6 months), leading to carbon enrichment. The DOC content of leaf litter decreased with the extension of decomposition time, and also exhibit carbon enrichment in small degree during the middle to late stages of decomposition (12-18 months) or the late stages of decomposition (18-24 months). Mixed leaf litter showed that the higher the proportion of broad leaves, the lower the DOC content in the later stage. (2) The antagonistic effect of DOC release rate in the early stage of decomposition (0-6 months) was strong (58.33%), and its synergistic effect gradually strengthened (91.67%) after 6-18 months. But the synergistic effect of leaf litter weakened (66.67%) during the late stage of decomposition (18-24 months). Among all mixed treatments, PT64 exhibited synergistic effects throughout the entire decomposition period, followed by PT73, PCT622 and PCT613(3/4). (3) Partial Least-Squares Regression (PLS) showed that N content, P content, lignin content, condensed tannin content, C/N, C/N, lignin/N, and lignin/P were important factors affecting the release of DOC from litter in this study area. Overall, the DOC release from mixed P. massoniana and broad-leaved litter was affected by tree species, mixing ratio and decomposition time. Among all of the mixtures, PT64, PT73, PCT622 and PCT613 showed strong synergistic effects for DOC release.
Subjects: Biology >> Biochemistry Subjects: Biology >> Molecular Biology submitted time 2024-01-02
Abstract： Objective: Comparison of urine proteome between obese people and normal people.
Methods: Urine samples from obese and normal people were collected and identified by non-label quantitative proteomics using high performance liquid chromatography tandem mass spectrometry (LC-MS/MS). The difference proteins of urine proteome between obese and normal people were screened for protein function and biological pathway analysis. The urine proteome of obese individuals was compared with that of normal people, and the common differential proteins were counted to analyze the protein function and biological pathways. Reported biomarkers of obesity were searched in the urine proteome of obese individuals.
Results: 38 different proteins can be identified in the urine proteome of obese people compared with normal people, some of which have been reported to be related to metabolism and obesity, and the biological processes of differential proteins are also related to metabolism and other processes. 8 common differential proteins in the urine proteome of obese individuals and normal people, among which some proteins have been reported to be related to metabolism and obesity, and the biological processes of differential proteins are also related to metabolism and other processes. Among the differential proteins in the urine proteome of obese individuals compared with the normal people, the reported obesity biomarkers can be matched.
Conclusions: The urine proteome can distinguish the obese people, and the differential proteins in the urine proteome have key proteins that are known to be related to obesity and metabolism, and the biological processes of differential proteins also related biological processes such as nutrition and metabolism. Urine proteome has the potential to explore the pathogenesis of obesity and provide personalized treatment.
Peer Review Status:Awaiting Review
Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》
Abstract： The Quercus subsect. Campylolepides contains three species: Q. acutissima Carruth., Q. variabilis Blume and Q. chenii Nakai, which is the East Asian clade of Quercus section Cerris. Species formation and phylogeography of whole subsection or species within the subsection have been studied in detail. It was also found that the Q. section Cerris had an ancient gene introgression with the Q. section Ilex, which led to the plastid capture. However, the specific evolutionary history of plastids in Q. subsect. Campylolepides and Q. section Ilex remains unclear. Our study performed low-coverage whole genome sequencing on 15 samples from Q. section Ilex and integrated previously published data from Q. subsect. Campylolepides and its relatives, resulting in a total of 325 resequencing data, of which 276 individuals were from three Q. subsect. Campylolepides species and 19 populations. We assembled 325 plastids to perform phylogeographic analysis. The results were as follows: (1) There are shared haplotypes among three species of Q. subsect. Campylolepides, but the plastid haplotypes of the entire Q. Subsect.Campylolepides form a monophyletic branch nested within the Q. section Ilex species which are distributed from central China to Liangshan, Sichuan. (2) A relict haplotype of Q. subsection. Campylolepides from the Liaodong Peninsula clustered with Q. pseudosetulosa Q.S.Li & T.Y.Tu, a specie of the Q. section Ilex. (3) Both plastid capture events occurred in the middle Miocene, after which no plastid capture occurred between Quercus Subsect. Campylolepides and Quercus section Ilex. It is hypothesized that Q. subsect. Campylolepides and Q. section Ilex have formed almost complete reproductive isolation.
Subjects: Biology >> Biophysics Subjects: Chemistry >> Physical Chemistry Subjects: Biology >> Biophysics submitted time 2024-04-16
Abstract： Transcription factor (TF) are proteins that regulates the transcription of genetic information from DNA to messenger RNA by binding to a specific DNA sequence. Nucleic acid-protein interactions are crucial in regulating transcription in biological systems. This work presents a quick and convenient method for constructing tight-binding models and offers physical insights into the electronic structure properties of transcription factor complexes and DNA motifs. The tight binding Hamiltonian parameters are generated using the random forest regression algorithm, which reproduces the given ab-initio level calculations with reasonable accuracy. We present a library of residue-level parameters derived from extensive electronic structure calculations over various possible combinations of nucleobases and amino acid side chains from high-quality DNA-protein complex structures. As an example, our approach can reasonably generate the subtle electronic structure details for the orthologous transcription factors human AP-1 and Epstein-Barr virus Zta within a few seconds on a laptop. This method potentially enhances our understanding of the electronic structure variations of gene-protein interaction complexes, even those involving dozens of proteins and genes. We hope this study offers a powerful tool for analyzing transcription regulation mechanisms at an electronic structural level.
Peer Review Status:Awaiting Review
Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》
Abstract： Primulina pingguoensis H.S. Ma & B. Pan, a new species of Gesneriaceae from Guangxi Zhuang Autonomous Region, China, is illustrated and described here. The new species morphologically resembles Primulina carinata Y.G. Wei, F. Wen & H.Z. Lü, but it differs from the latter by lobes narrowly lanceolate-linear，length-width ratio >2 (rounded-ovate，length-width ratio <1.5), 8–10 purple stripes from corolla throat to the bottom of corolla tube, without honey guides (vs. brown stripes, 2 yellow honey guides inside, leaf blade elliptic to broadly ovate, 6.5–9.5 × 4.5–6.5 cm (vs. broadly elliptic to ovate, 4.0–5.0 × 3.0–4.0 cm), leaf blade base slightly cuneate (vs. rounded), corolla tube tubular, ventrally carinate, (vs. narrowly funnelform, strongly carinate, forming a clear keel), etc. The conservation status of P. pingguoensis is considered as ‘Critically Endangered’ (CR) according to the IUCN red list categories and criteria.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-26 Cooperative journals: 《广西植物》
LIANG Huizi
YANG Jisheng
QIN Yi
PAN Weihu
XIAO Yufei
HUANG Ronglin
WANG Renjie
JIANG Yi
LIU Xiongsheng
Abstract： Hopea chinensis is distributed in China and its seeds are typical stubbornness. In order to investigate the germination adaptability of seeds to temperature and moisture, we carried out an experiment with three different constant temperature (15 ℃, 20 ℃ and 25 ℃) controlled by artificial climate incubators, and six polyethylene glycol (PEG-6000) mass percentage concentrations (0, 5%, 10%, 15%, 25% and 35%) were used to simulate drought stress. The germination characteristics of these seeds were carefully examined. The results were as follows: (1) Temperature exerted a significant influence on seed germination. Regardless of the level of drought stress applied, higher temperatures positively affected the germination rate, germination energy, germination index, radicle length, seeding length and vitality index of seeds, higher temperatures led to shorter germination delay and increased fluctuations in the germination period. (2) Drought stress had a significant impact on seed germination. Under identical temperature conditions, increasing levels of drought stress resulted in reduced germination rate, germination energy, germination index, germination period, radicle length, seeding length and vitality index of seeds. Moreover, higher levels of drought stress led to longer in germination delay of seeds. (3) The interaction between temperature and drought stress had a significant influenced on seed germination. Under drought stress, seed germination exhibited different responses under different temperature conditions. Specifically, under conditions where a PEG-6000 concentration of 35% was applied as drought stress treatment, the germination rates at temperatures of 20 °C and 25 °C were significantly higher than those observed at 15 °C (8.89% and 15.55% compared to lower rates at 15 °C). However, it was important to note that when subjected to both 35% PEG-6000 and a temperature of 15 °C, the seeds no longer germinate. In summary, our results indicate that the suitable temperatures for seed germination are identified as being around 20 °C and 25 °C. It is determined that the most favorable temperature for early seedling growth is 25 °C. As the drought stress intensifies, the inhibitory effect on seed germination become stronger. These findings suggest that temperature plays a positive role in promoting seed germination, while drought stress can significantly hinder this process. Additionally, moderate warming can help alleviate the inhibitory effect of drought stress on seed germination.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》
Abstract： To explore the responses of physiological and biochemical and mechanism of molecular regulatory for grape dormancy breaking with cyanamide, Vitis vinifera×V. labrusca ‘Shuijing’ was used as experimental materials in this study to determine the changes in SOD, POD, CAT activity, MDA and H2O2 content, and oxygen free radical production rate, and RT-PCR technology was used to clone the full-length cDNA sequences of two FT (Flowering location T) genes (VvFT1 and VvFT2) and one CBF (C-repeat binding factor) gene (VvCBF) from its buds, then their physicochemical properties, phylogenetic evolution, conserved motifs and domains, and differences expression levels in grape buds after cyanamide treatment were analyzed. The results were as follows: (1) The analysis of physiological and biochemical indicators results showed that SOD, POD and CAT activities, MDA and H2O2 contents, and the rate of oxygen free radical production in grape buds were significantly increased after treated with cyanamide. (2) The full-lengths cDNA sequences of VvFT1 and VvFT2 genes were 525 bp from Vitis vinifera×V. labrusca ‘Shuijing’, encoding 174 aa, and the full-lengths cDNA sequences of VvCBF gene was 714 bp, encoding 237 aa. (3) The homology analysis results showed that VvFT1 of Vitis vinifera×V. labrusca ‘Shuijing’ had the highest amino acid homology with Litchi chinensis (LcFT: AEU08960.1) and Dimocarpus longan (DlFT2: ALA55998.1), VvFT2 had the highest amino acid homology with LcFT (AEU08961.1) and DlFT2 (AHF27444.1). The phylogenetic analysis result showed that VvFT1, VvFT2, LcFT (AEU08960.1; AEU08961.1) and DlFT2 (ALA55998.1; AHF27444.1) clustered into a branch, with the most closes genetic relationship; VvCBF had the highest amino acid homology with Prunus ledebouriana (PlCBF: AEB69782.1), and the phylogenetic analysis showed that VvCBF and PlCBF clustered into a branch, with the most closest genetic relationship. (4) qRT-PCR analysis showed that VvFT1 and VvFT2 expression levels were significantly increased in buds after treated with cyanamide, while VvCBF expression levels was significantly decreased. In summary, this study comprehensively analyzed the phylogenetic evolution of VvFT1, VvFT2, and VvCBF genes, as well as the expression patterns of these genes and physiological and biochemical indicators in grape buds after treated with cyanamide, laying a theoretical foundation for the molecular and physiological mechanisms of grape dormancy breaking with cyanamide.
Subjects: Physics >> Nuclear Physics Subjects: Biology >> Radiobiology submitted time 2024-05-06
Abstract： Manned spaceflight and nuclear technology applications are running on a highway in China today. The radiation and nuclear safety will continue to be a major national demand in a long term. Thus, the continuous observation of new radiation protection molecular targets and related drugs is of great value to us. Our previous study has found that the circulating Insulin-like Growth Factor Binding Protein 3 (IGFBP-3) showed a significant increase after total body exposure of mice to ionizing radiation. However, the function of IGFBP-3 and the effects of it level change on radiation induced damages are still unclear. In this study, we set up the Igfbp3 gene overexpression and knock-down cell models in mouse Kupffer (MKC) cells. The CCK-8 assay, EdU assay, clone formation assay and microsphere phagocytosis experiment were performed for investing the proliferation activity, DNA replication activity and phagocytic ability of different cell models after carbon-ion irradiation. Moreover，mice were tail vein injected with recombinant IGFBP-3 protein at 2 hours before 5 Gy carbon-ion irradiation, and the survival curves of mice were drawn. The results showed that overexpression of IGFBP-3 protein significantly alleviated the radiation-induced decrease of the DNA replication activity, cell viability, clone formation rate, and phagocytic ability of MKC cells. On the contrary, the knock-down of IGFBP-3 protein expression reduced the above results. Injection of IGFBP-3 protein before carbon-ion exposure significantly delayed the time of death in mice. Our results indicate at the cellular and animal levels that IGFBP-3 protein has the potential to reduce radiation-induced damages and serve as a target for radiation protection. Through enhancing the radiation resistance and phagocytic ability of Kupffer cells in mice to reduce the risk of infection after radiation exposure might be the underlying mechanism of the effects of IGFBP-3 on radiation protection.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-07-13 Cooperative journals: 《广西植物》
Abstract： Parakmeria omeiensis an evergreen tree belonged to Parakmeria of Magnoliaceae, is listed as a critically endangered plant under the first-grade State protection. Aimed to clarify the abortion period of male sterility in bisexual flowers of P. omeiensis and its physiological and biochemical characteristics, sterile stamens and fertile stamens were test materials, the anther development was observed by paraffin section, the contents of soluble sugar, soluble protein, proline were determined, and catalase ( CAT) and peroxidase ( POD) activity were analyzed. The results were as follows: (1)Significant difference were detected in meiosis stage in anther development between sterile stamens and fertile stamens, the tapetum layer of sterile stamens was dense and undeveloped, the microspore mother cells didn’t develop into the tetrad and subsequently disintegrated, so the pollen sac was empty. The tapetum layer and the microspore mother cells of fertile stamens developed normally, most pollen sac were broken and pollen grain were export. (2)The contents of soluble sugar, soluble protein, proline of sterile stamens were significantly lower than fertile stamens during the stage of meiosis, microspore monuclear and pollen mature. (3) POD activity of sterile stamens increased overall and was significantly higher than fertile stamens. CAT activity of sterile stamenst decreased as a whole and was significantly lower than fertile stamens. We conclude that the microspore abortion occurs at the stage of meiosis, the decrease in metabolism of material and energy causes the abortion of male in bisexual flowers, the tapetum layer don’t develop further and it doesn’t provide nutrients to the microspore mother cells. CAT and POD activity are abnormalwhich results in the failure of timely scavenging free radicals in cells, and meiosis of microspore mother cells is blocked, so tetrads fail to be formed.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》
Abstract： To explore the effects of nitrogen, phosphorus and potassium on the physiology of Pyrrosia petiolosa as well as the synthesis and accumulation of chlorogenic acid, tissue culture seedlings of P. petiolosa were used as materials, and three concentration gradients of low nutrient ( no fertilization : N0, P0, K0 ), normal fertilization ( N : 0.2 g·kg-1, P : 0.15g·kg-1, K : 0.15 g·kg-1 ) and high nutrient ( N1 : 0.4 g·kg-1, P1 : 0.3 g·kg-1, K1 : 0.3 g·kg-1) were set up. There were seven treatments set up: NPK、N0PK、N1PK、NP0K、NP1K、NPK0 and NPK1, and the resistance physiological indexes, chlorogenic acid content and key enzyme activities of P. petiolosa under different treatments were determined. The results were as follows: (1) Nitrogen, phosphorus and potassium fertilizers had a significant effect on the resistance physiology of P. petiolosa. The activity of superoxide dismutase(SOD) increased significantly under high nitrogen and low potassium treatments, but catalase (CAT) activity rose significantly under low and high treatments of the three nutrients. (2) The content of chlorogenic acid in P. petiolosa was significantly affected by different levels of nitrogen, phosphorus, and potassium. The chlorogenic acid level in normal fertilization was the highest, reaching 12.92 mg·g-1, while the chlorogenic acid content in high potassium fertilization was the lowest, 7.79 mg·g-1. Potassium fertilizer had the most significant effect on chlorogenic acid content. The content of chlorogenic acid was positively correlated with the activities of quinate o-hydroxycinnamoyl transferase (HQT) and coumaroyl coenzyme ligase (4CL), and negatively correlated with the activity of shikimate o-hydroxycinnamoyl transferase (HCT). HQT, 4CL and HCT were the key factors leading to the difference of chlorogenic acid content. The results of this study can provide a theoretical basis for the artificial cultivation of P. petiolosa.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》
Abstract： To explore the feasibility of constructing a tobacco mutant library using the CRISPR/Cas9 gene editing technology, the study designed sgRNAs of 100 aroma related genes in tobacco, constructed a plasmid library composed of 100 corresponding CRISPR/Cas9 editing vectors, and analyzed the co-transformation rate, target editing efficiency, and off-target editing rate of transgenic offspring. The results were as follows: (1) After co-transformation of 100 sgRNAs mediated by Agrobacterium tumefaciens, 77 of them were detected in 172 positive transformation strains, with a co-transformation efficiency of 77%; (2) Among 77 transgenic offspring carrying sgRNA, 69 sgRNAs edited the target genes, with an editing efficiency of 89.6%; (3) Sequencing detection revealed that only one sgRNA produced off-target editing at a non-target site, indicating a very low probability of off-target editing of CRISPR/Cas9 in tobacco. In summary, it is feasible to construct a mutant library by the co-transformation of a CRISPR/Cas9 vector library to edit a large number of candidate target genes in tobacco. This method has the characteristics of the high co-transformation efficiency, the high editing efficiency, and the low probability of off target editing.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》
Abstract： In order to grasp the spatial temporal variation characteristics and driving mechanism of vegetation ecological quality in Guangxi, based on multi-source data such as meteorology, terrain, soil and remote sensing, and using the ecological quality index (EQI) as an evaluation indicator, linear trend analysis, correlation analysis and geographical detector method were used to analyze the spatiotemporal changes and driving mechanisms from 2000 to 2020. The results were as follows: (1) Since 2000, the vegetation ecological quality index of Guangxi has significantly increased, and the regional vegetation ecology has improved significantly.The development of vegetation ecological quality has experienced stages of slow growth, rapid growth and significant improvement. In terms of space, the vegetation ecological quality index in Guangxi shows a characteristic of high in four directions and low in the middle, with the high-value areas gradually expanding from the east to the west and north.(2) The influencing factors of spatial and temporal evolution of vegetation ecological quality in Guangxi were significantly different.The overall change of vegetation ecological quality at different altitudes showed a trend of ‘increase-decrease-stability-fluctuation’. The vegetation ecological quality in different soil types is high in loam soil and low in sandy soil. The ecological quality of forest and shrub-grass is high, and the ecological quality of farmland vegetation is low. There is a significant positive correlation between vegetation ecological quality and climate driving factors, which is affected by both temperature and precipitation.The area mainly driven by temperature (T) is the widest, followed by the area mainly driven by precipitation (P). The areas driven strongly ([T+P]+) and weakly ([T+P]-) by temperature and precipitation are smaller.(3) The driving force of changes in vegetation ecological quality change of Guangxi was affected by terrain, soil, vegetation, climate, natural disasters and human activities. The order of explanatory power of natural influencing factors was vegetation > terrain > soil > climate, in which the vegetation net primary productivity and vegetation coverage were the most important factors affecting the spatiotemporal differentiation of vegetation ecological quality. The effects of natural factors on vegetation ecological quality in Guangxi was interactive, showing a nonlinear enhancement and two-factor enhancement relationship, and the interaction between terrain and vegetation, soil and vegetation, and climate and vegetation were the most obvious. Natural disasters and human activities aggravated the impact of vegetation ecological quality change. Among them, meteorological disasters such as drought and low temperature damage inhibited the improvement of vegetation ecological quality, while human activities such as forestry ecological engineering promoted the improvement of vegetation ecological quality. The research results provided scientific theoretical basis and technical support for making reasonable measures of vegetation ecological protection and restoration in Guangxi.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》
LUO Yahuang
MA Liangliang
GAO Lianming
WANG Xinjie
ZHAO Wei
YANG Xingliang
MA Shaobin
SHI Xiaochun
LIU Jie
Abstract： A better understanding of the spatiotemporal dynamics of species composition and biodiversity provides significant insights into the distribution patterns of biodiversity and the prediction of biodiversity fate in a global change context. However, how species and phylogenetic diversity change along elevational gradient with temporal scale in mountain ecosystems is largely unknown. In this study, we used census data (2004, 2008 and 2013) of an elevational transect (elevational range 960-2 878 m) covering eastern and western slopes of Gaoligong Mountains, SW China, to investigate the spatiotemporal dynamics of forest arbor layer (DBH≥5 cm) species diversity and phylogenetic diversity. The results were as follows: (1) Species diversity was humpshaped along the elevational gradient, while the phylogenetic diversity increased with increasing elevation. Clustering phylogenetic structure was observed at low elevations, and overdispersed or random structure was detected at high elevations. (2) Overall, temporal species and phylogenetic beta diversity analysis showed no significant difference among these three investigation time points. However, we found a higher clustering of phylogenetic structure along the temporal scale. (3) Spatial beta diversity showed that there was a significant loss of species at low elevations (960-1 381 m) on the eastern slope, where the vegetation has been disturbed or completely converted into farmland. The main lost species including Terminalia chebula, Quercus acutissima,Pistacia weinmanniifolia, Hovenia acerba and Ilex wattii. In contrast, the change in the western slope is mild at low elevations, only observing species abundance increasing, which include Cyclobalanopsis oxyodon, Symplocos chinensis, and Taiwania cryptomerioides. Therefore, we speculate that the change in species and phylogenetic diversity of the arbor layer of subtropical evergreen broadleaf forest may be related to succession process and climatic change in middle and high elevations, while the communities at low elevations were seriously impacted by human activities in the Gaoligong Mountains. The results will deepen the understanding of the mechanisms of plant community dynamics in the Gaoligong Mountains and provide new insights to guide precise conservation strategies in the region.
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