Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》
Abstract： Effects of different nitrogen fertilizer application rates on fruit quality of pitaya under the condition of calcareous soil in karst area were discussed in order to promote the growth, development and quality improvement of pitaya. The ‘Taiwan Dahong’ pitaya in 3-year-old were used as the experiment material. Based on 0.216 kg of phosphorus (P) and 0.324kg of potassium (K), four nitrogen application levels (CK, T1, T2, T3) were setted. 21 fruit quality indexes were determined, and the quality differences of pitaya with different nitrogen application levels (CK,T1,T2,T3) were compared. At the same time, 11 appearance quality indexes were comprehensicely analyzed by principal component analysis. The results were as follows: (1) Compared with the control, nitrogen application could increase the soluble sugar content and soluble solids of fruit content, reduce the protein content and dietary fiber content, and the high nitrogen treatment (T3) was significantly different from other treatments. With the increase of nitrogen application rate, the titratable acid content and vitamin C content of fruit increased first and then decreased, while the solid-acid ratio decreased first and then increased with the increase of nitrogen application rate, and the high nitrogen treatment(T3) was significantly higher than other treatments. High nitrogen level significantly increased the fruit vertical and horizontal diameter, fruit shape index and single fruit weight. (2) Compared with the control, nitrogen application treatment reduced the N content of pitaya fruit and increased the P content, and the N and P contents of each treatment were significantly different. The K content of fruit increased with the increase of nitrogen application rate, and T3 treatment was significantly higher than other treatments. Nitrogen application increased fruit B content and decreased fruit Cu content. The contents of Mn, Fe, Ca and Mg in different treatments were ranked as T3 > T1 > CK > T2. The content of Zn decreased first and then increased with the increase of nitrogen application rate. (3) The principal component comprehensive evaluation score of the high nitrogen level treatment was the highest, indicating that when selecting brown calcareous soil in Guilin karst area to grow pitaya, supplemented by a certain amount of P and K fertilizer, high nitrogen treatment was more conducive to improving the quality of pitaya. In actual production, it is necessary to make appropriate adjustments according to the soil fertility status of the plantation.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-12-19 Cooperative journals: 《广西植物》
Abstract： Different varieties have different resistances. In order to further explore the resistance differences in different varieties of pitaya fruit and provide a reference for further study on breeding of pitaya fruit resistance. Our study using Illumina HiSeq 2000 sequencing platform to sequencing the transcriptome of ‘Putongbairou’ （BR） and ‘Ecuador Yellow’ （EY）. Functional classification and enrichment analysis of differentially expressed genes （DEGs） were performed by reference to GO Ontology, KEGG and others databases. The results were as follows: (1) There were 14 248 DEGs between BR and EY, of which 5 446 genes were up-regulated and 8 802 genes were down-regulated. (2) GO functional analysis showed that these DEGs are mainly involved in enzyme catalytic activity, cell components, metabolic processes, etc. Among them, there were 349 differential genes involved in oxidoreductase activity. (3) KEGG pathway analysis showed that most of the DEGs were enriched in metabolism, biosynthesis, etc. , and 12 key genes such as CYP86 and CER1 involving in Cutin, suberine and wax biosynthesis. We found the expression of DEGs involved in oxidoreductase activity were higher in BR than that in EY, which significantly enriched, indicating that may be differences in growth and cell metabolism between BR and EY. DEGs involved in the biosynthesis of Cutin, suberine and wax biosynthesis were up-regulated in BR. Such genes have higher expression in BR, and significantly enriched, suggesting that BR may be superior drought and disease resistance than EY.
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