Subjects: Biology >> Botany >> Applied botany submitted time 2021-11-04 Cooperative journals: 《广西植物》
Abstract： Stephania kwangsiensis, a vine plant of the Stephaniaceae family, is mainly spread in Guangxi, China. The roots of S. kwangsiensis, “Jin Bu Huan” in Chinese, is often used for sedative, insecticidal, anti-inflammatory, anti-tumor, antiviral, anti-bacterial, as well as for the treatment of Alzheimer’s disease. In order to make full use of the title plant resources, clarify its chemical components, and find the relevant active compounds, the methanol extracts of non-medicinal parts of this plant was isolated using various chromatographic methods such as positive phase Sephadex LH-20, positive phase silica gel, positive phase polyamide, and MCI gel CHP 20P. The structures of the isolated components were determined by MS, 1H NMR, 13C NMR, and other spectroscopic data analysis, as well as comparison with relevant literatures. The activities of the isolated compounds from S. kwangsiensis against bacteria (Escherichia coli, Staphylococcus aureus, Bacillus subtilis) and fungi (Fusarium oxysporum) were evaluated using filter paper method. The results were as follows: (1) 10 compounds obtained from the non-medicinal parts of this plant were identified as vomifoliol (1), grasshopper ketone (2), isocorydine(3),2-O-β-D-glucopyranosyl-D-arabinitol(4),(9S,12S,13S)-trihydroxyoctadeca-(10E,15Z)-dienoic acid (5), magnoflorine (6), corchoionoside C (7), tormentic acid (8), corydine (9), and crebanine (10). Among of them, compounds 1, 2, 4, 5, 6, 7 and 8 were isolated from this plant for the first time. Compounds 1, 2, 4, 5, 7 and 8 were firstly obtained from this genus plants. And compound 4 was found from natural products for the first time. (2) At concentration of 6 mg·mL-1, Compounds 1, 2 and 5 show weak antibacterial activities against Escherichia coli; Compound 2 exhibits weak antibacterial activities against Staphylococcus aureus; Compounds 3 and 8 display weak antibacterial activities against Bacillus subtilis. The non-medicinal parts of Stephania kwangsiensis contains large numbers of active components and have the potential of development and utilization.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-05-27 Cooperative journals: 《广西植物》
Abstract： This paper investigates the modulating effect of total tomato saponin(TTS) on uric acid. In this articles, we used hypoxanthine and potassium oxyzincate or uric acid and potassium oxyzincate to establish hyperuric acid model mice, and investigated the effects of TTS on uric acid excretion, blood uric acid, urea nitrogen, creatinine, xanthine oxidase and organ index in normal mice and hyperuricemic mice. The experimental results were as follows: TTS did not affect the blood uric acid level in normal mice, and the blood uric acid values in the normal group and the low, medium and high dose groups of TTS were（170.4±36.7） ,（178.3±69.7）,（175.5±42.1）,（185.3±72.6 ）μmol·L-1, respectively. TTS could reduce blood uric acid level and xanthine oxidase activity in hypoxanthine and potassium oxyzinate combination-induced hyperuricemic mice, and the blood uric acid values in normal group, model group and TTS high dose group were:（140.4±36.7）,（378.3±69.7）,（278.3±62.6）μmol·L-1, respectively. The xanthine oxidase values in the normal, model and TTS low, medium and high dose groups were（1.2±0.3）,（1.8±0.2）,（1.6±0.2）,（1.5±0.3）,（1.3±0.4）U·g-1liver, respectively. TTS reduced blood uric acid levels and decreased xanthine oxidase activity in mice with hyperuricemia induced by the combination of uric acid and potassium oxyzinate, and the blood uric acid values in normal group, model group and TTS high dose group were:（98.8±21.8）,（455.6±78.8）,（333.7±68.7）μmol·L-1, respectively. The xanthine oxidase values in the normal, model and TTS high dose groups were: （2.1±0.3）,（2.5±0.2）,（2.3±0.2）U·g-1liver, respectively. The above results suggest that TTS does not affect blood uric acid levels in normal mice, but can reduce blood uric acid levels in mice in a hyperuric acid model, and the mechanism may be related to the reduction of xanthine oxidase activity.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-04-29 Cooperative journals: 《广西植物》
Abstract： Flavonoid-C- glycosides, as one of the effective components in Premna fulva, have the effect of antigen-induced arthritis. The enrichment and purification process of five flavonoid-C-glycosides as in P. fulva stems is preferred. Through static adsorption and desorption experiments on 13 kinds of macroporous resins with five flavonoid-C- glycosides in P. fulva stems as the index,a suitable macroporous resin was selected, and the results were tested by the HPLC, and then the orthogonal process was used to optimize the enrichment and purification conditions. The results showed that XAD-16N macroporous resin has a good adsorption and desorption effect on the five flavonoid -C-glycosides in P. fulva stems. Through single factor screening and orthogonal process optimization, the best enrichment and purification process is finally determined as follows: the loading concentration is 0.5 times the original extract diluted, the loading flow rate is 2 BV穐-1, and the eluent concentration is 60 % ethanol. The content of five flavonoid-C-glycosides increased from 1.73 % (in the original plant) to 14.5 %, an increase of 7.38 times. The results showed that the selected extraction process is stable and feasible, and the selected macroporous resin has a good separation and purification effect, which provides a reference for the drug development of P. fulva stems.
Subjects: Biology >> Botany >> Applied botany submitted time 2020-09-15 Cooperative journals: 《广西植物》
Abstract：建立HPLC法同时测定大果山楂果实中原儿茶酸、绿原酸、二氢咖啡酸、咖啡酸、根皮酸、p-香豆酸、阿魏酸和肉桂酸的方法，并分析这8种酚酸在不同产地大果山楂果实中的含量。采用ZORBAX SB-C18色谱柱，以甲醇/1.5%甲酸水溶液（v/v）为流动相，梯度洗脱，柱温35 ℃，流速1.0 mL•min-1，检测波长280 nm、320 nm。结果表明：在线性范围内8种酚酸质量浓度与色谱峰峰面积的线性关系良好，相关系数均＞0.997，检出限0.08~0.20 μg•mL-1，定量下限0.27~0.67 μg•mL-1，变异系数均<5.0%，加标回收率99.3%~103.3%；10个不同产地的大果山楂果实中酚酸含量丰富，均检出原儿茶酸、绿原酸、二氢咖啡酸、咖啡酸、根皮酸、阿魏酸和肉桂酸7种酚酸，其中二氢咖啡酸、根皮酸、阿魏酸首次检出，以绿原酸为主（8 410.2~13 826.7 μg•g-1），占总酚酸的80%以上，总酚酸的质量分数在10 187.8~15583.9 μg•g-1之间，其中广西百色靖西和桂林恭城产的果实总酚酸质量分数相对较高，均大于15 000 μg•g-1。本研究的HPLC法适用于大果山楂果实中酚酸含量的测定，能为大果山楂优良品种的选育、产品质量控制及深度开发利用提供方法和科学参考。
Subjects: Biology >> Botany >> Applied botany submitted time 2019-06-17 Cooperative journals: 《广西植物》
Abstract：植物内生真菌与宿主植物互惠共生，可提高宿主植物的营养吸收、抗逆境能力以及刺激宿主植物活性物质的产生，是植物病害生物防治领域的重要研究内容之一。植物内生真菌种类繁多，通过ITS 基因序列扩增和测序分析，结合传统的形态学鉴定方法，可快速、有效鉴定内生真菌。本文采用传统形态学方法结合rDNA-ITS 序列分析，对我国重要药用植物罗汉果中的内生真菌进行鉴定，并研究其多样性，以期为后期探究内生真菌与罗汉果互作关系奠定基础。采用组织培养法分离内生真菌，从罗汉果健康植株中共分离得到150 株内生真菌，包括罗汉果雌株中的内生真菌96 株和罗汉果雄株中的内生真菌54 株。其中，122 株内生真菌经形态学结合rDNA-ITS 序列分析鉴定为9 个属，均归属为子囊菌门，包含座囊菌纲（Dothideomycetes）和子囊菌纲（Sordariomycetes）。其中座囊菌纲（Dothideomycetes）真菌包含3 科、3 属；子囊菌纲（Sordariomycetes）真菌包含6 科、6 属。优势属为刺盘孢属（Colletotrichum）和镰刀菌属（Fusarium）。罗汉果雌、雄植株不同组织中内生真菌的定殖率及分离率的变化规律均不相同，雌株中以根中内生真菌的定殖率和分离率最高，叶片中的最低；而在雄株中以叶片中的定殖率和分离率最高，根中的最低。另外，不同菌株在雌、雄两种植株的不同组织中的分布均不同，结合内生真菌群落组成的相似性比较结果，表明部分内生真菌对罗汉果雌株和雄株，以及同一植株中的不同组织均具有偏好性。不同组织中内生真菌的多样性指数在0.11-0.69 的范围，其中雌株根部的内生真菌多样性指数最高。
Subjects: Biology >> Botany >> Applied botany submitted time 2019-03-14 Cooperative journals: 《广西植物》
Abstract：为了明确展毛野牡丹的化学成分，运用Diaion HP20SS、MCI、Sephadex LH-20柱层析和反相高效液相色谱等方法对展毛野牡丹根和茎的醇提物分别进行分离纯化，从中分离得到11个化合物，经波谱数据的分析，鉴定了它们的结构。它们是4-羟基-3-甲氧基苯酚1-O-β-D-(6'-O-没食子酰)-吡喃葡萄糖苷（1）、3,4-二羟基苯乙醇4-O-β-D-(6'-O-没食子酰基)-吡喃葡萄糖苷（2）、龙胆酸5-O-β-D-(6'-O-没食子酰基)-吡喃葡萄糖苷（3）、2,4,6-三甲氧基苯酚1-O-β-D-(6'-O-没食子酰)-吡喃葡萄糖苷 (4)、甲基6-O-没食子酰基-β-D-吡喃葡萄糖苷（5）、乙基6-O-没食子酰基-β-D-吡喃葡萄糖苷（6）、6′-O-没食子酰基黑樱苷（7）、没食子酸甲酯（8）、没食子酸乙酯（9）、2,6-二甲氧基对苯二酚4-O-β-D-吡喃葡萄糖苷（10）、 2-甲氧基对苯二酚4-O-β-D-吡喃葡萄糖苷（11）。所有化合物均为首次从展毛野牡丹中分离得到，化合物2-7、10和11为首次从该属植物中分离得到。
Subjects: Biology >> Botany >> Applied botany submitted time 2018-10-26 Cooperative journals: 《广西植物》
Abstract： Leersia hexandra Swartz is the chromium hyper-accumulative plant which was found firstly in China. The endophytic bacteria of L. hexandra Swartz and their removal capacity for Cr(VI) were studied in this paper. A Cr(VI)-resistant endophytic bacterium G04 was isolated from the roots of L. hexandra Swartz by the solid plate culture method using the media of beef extract peptone containing Cr(VI). Biological identification results showed the strain belonged to Enterobacter cloacae. Effects of culture conditions, such as initial pH, temperature, Cr(VI) concentration, liquid volume, inoculation amount, shaking speed and culture time, on the removal rate of Cr(VI), removal rate of total Cr and the growth of the strain were studied in detail using the method of shaking flask culture. The results showed that the optimal conditions for the removal of Cr(VI) by E. cloacae G04 were as followed: initial pH of 5.0, culture temperature of 37 ℃, substrate concentration of 100 mg·L-1, liquid volume of 80 mL in 250 mL conical flask, inoculum size of 15%, shaking speed of 100 r·min-1 and culture time of 48 h.Under these conditions, the removal rate of Cr(VI) and total chromium were about 84% and 8%, respectively. The results of this study show that the endophytic bacteria Enterobacter cloacae G04 has better application potential for removing chromium. It may be used directly for remediation of soil and water environment contaminated of chromium, and also may be used as alternative strain for promoting plant remediation of chromium pollution. Furthermore, the result has an important reference value to illuminate the mechanism of chromium hyper-accumulation of L. hexandra Swartz.
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