Subjects: Biology >> Botany >> Applied botany submitted time 2019-08-26 Cooperative journals: 《广西植物》
Abstract： The chemical constituents of essential oil of needles from Abies. ziyuanensis and A. yuanbaoshanensis were compared to provide a theoretical basis for the development and utilization of this two firs. Essential oil of needles were extracted by steam distillation, and were determined by GC-MS analysis. The relative content of each component was calculated based on the peak area normalization method. The results show that 21 and 22 kinds of chemical substances were identified in the essential oil of needles from A. ziyuanensis and A. yuanbaoshanensis, respectively. Among which 15 kinds chemical substances were the common components of this two firs; The Olefinic substances were the highest content in essential oil of needles from both A. ziyuanensis and A. yuanbaoshanensis, the proportion were 95.94 % and 95.02 %, respectively. Among the common components of two firs, β-Pinene, l-Caryophyllene, Camphene, a-Caryophyllene and Terpinolene have high development and utilization value; in terms of non-common components, a-Terpineol and a-Pinene in A. ziyuanensis both a-Muurolene and Leaf alcohol in A. yuanbaoshanensis had great development and utilization value.
Subjects: Biology >> Botany >> Applied botany submitted time 2019-06-17 Cooperative journals: 《广西植物》
Abstract：为探究罗汉果(Siraitia grosvenorii) 自然授粉不良的形成原因，对其雌株和雄株的访花昆虫及叶片的挥发性成分进行了比较分析。采用定点定时方法调查罗汉果雌雄株访花昆虫，同时以GC-MS 分析雌雄株叶片的挥发性成分。结果表明：在雄株观察到访花昆虫102 种，分属8目29 科，其中包括了蜜蜂科、眼蝶科、夜蛾科和天蛾科等传粉昆虫类群；在雌株观察到访花昆虫69 种，分属7 目16 科，但未观察到上述传粉昆虫类群。雄株访花昆虫的物种丰富度、多度和Shannon-Wiener 多样性均显著高于雌株(P < 0.05)，Jaccard 相似性分析显示雌株和雄株访花昆虫达到中等不相似水平。在雄株叶片中鉴定出挥发性成分17 种，优势成分为萜烯类化合物，占总含量的67.31%；雌株叶片则鉴定出挥发性成分12 种，优势成分为烷烃类化合物，占总含量的44.27%。雄株具有较多特有成分，包括7 种萜烯类和3 种酯类成分，占总体成分的45.45%；雌株的特有成分较少，只包括4 种烷烃类和1 种酯类成分，占总体成分种类的22.72%。Jaccard相似性分析显示，雌株和雄株的挥发性成分总体上达到中等不相似水平，其中萜烯类和酯类的相似度更低，达到极不相似水平。进一步分析表明，在罗汉果雌雄株之间，由于挥发性化合物在优势成分上的重要差异，以及特有成分的大量存在，可能导致了它们访花昆虫类群的显著差异，并进而影响了罗汉果的自然传粉过程。
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