Subjects: Biology >> Botany >> Applied botany submitted time 2021-12-19 Cooperative journals: 《广西植物》
Abstract： In order to explore the response mechanism of AP2/ERF gene family in the water stress of Olea europaea, this study performed transcriptome sequencing on the roots and leaves of two cultivars 'Frantoio' and 'TYZ-1' that were under drought and flooding stress. And based on the whole genome data, the protein physicochemical properties, gene structure and system evolution of AP2/ERF transcription factor in O. europaea were analyzed. At the same time, the difference in gene expression of AP2/ERF transcription factor related to water stress in the two O. europaea cultivars was analyzed by transcriptome sequencing data and verified by fluorescence quantitative PCR. The results were as follows: (1) A total of 110 AP2/ERF gene family members were identified in O. europaea. The amino acid size of the 110 proteins is 173-717bp, there is no signal peptide and it is a non-secreted protein. The phylogenetic tree was constructed between O. europaea AP2/ERF and model plant Arabidopsis AP2/ERF protein. It was found that O. europaea AP2/ERF protein was divided into four categories: AP2, RAV, ERF and Solosist. Among them, ERF is divided into two subtypes: ERF and DREB. ERF includes six subtypes of ERF B1 to ERF B6, and DREB includes six subtypes of DREB A1 to DREB A6, which is consistent with the classification of the model plant Arabidopsis AP2/ERF. Each subfamily contains AP2/ERF proteins of O. europaea and Arabidopsis at the same time, indicating that the AP2/ERF family of Arabidopsis and O. europaea are similar in evolution. (2) The analysis of gene structure and conserved elements found that the proteins of the same subfamily of O. europaea AP2/ERF have the same gene structure and conserved elements. Combining gene expression with genes with known water regulation functions in the evolutionary tree, it is preliminarily speculated that OeAP2-75, OeAP2-97, OeAP2-101, OeAP2-23, OeAP2-13 are closely related to the water regulation of O. europaea, OeAP2-13, OeAP2-28, OeAP2-104, OeAP2-75, OeAP2-80, OeAP2-50 have different expression levels in the two varieties. It is speculated that this may be the reason for the different resistance of 'Frantoio' and 'TYZ-1'. The RT-qPCR technique was used to detect the expression changes of O. europaea AP2/ERF gene under different stresses. The results showed that OeAP2-101, OeAP2-28, and OeAP2-42 were significantly up-regulated by water stress, which was consistent with the results of transcriptome analysis. The results of this study can lay a foundation for the research on the stress resistance expression and gene function of the AP2/ERF family genes of O. europaea, and provide a method and theoretical basis for the selection of drought-resistant and flood-tolerant rootstock varieties of O. europaea.
Subjects: Biology >> Botany >> Applied botany submitted time 2019-08-26 Cooperative journals: 《广西植物》
Abstract： In order to reveal the molecular mechanism of terpenoid metabolism in Zanthoxylum armatum and the effect of grafting on its flavor, we designed specific primers based on transcriptome data and cloned a novel full-length cDNA sequence of geranylgeranyl pyrophosphate synthase (GGPPS) gene from Zanthoxylum armatum by RT-PCR. Named ZaGGPPS. The ZaGGPPS gene was analyzed by using NCBI, ProParam, SignalP 4.1 server, DNAMAN and MEGA7.0 software. The expression of ZaGGPPS gene in grafted and seedling trees was compared. The results showed that ZaGGPPS contains a complete open reading frame (OFR), consisting of 1 086 bp, encoding 361 amino acids. The relative molecular weight of the protein is 39 079.14 Da and the theoretical isoelectric point pI is 6.38. Blast comparison results showed that the protein belongs to the GGPPS family and contains two specific aspartic acid enrichment motifs, namely "DDXXXXD" and "DDXXD", and five characteristic functional domains. Phylogenetic tree results showed that Zanthoxylum armatum has close relationships with sweet orange (Citrus sinensis), clementine mandarin (C. clementina) and pomelo (C. maxima). Fluorescence quantitative PCR showed that the expression level of ZaGGPPS gene in Zanthoxylum armatum range from high to low as follows: leaf of seedling tree, leaf of grafted tree, stem of seedling tree and stem of grafted tree. Geranylgeranyl pyrophosphate synthase is a key enzyme in terpenoid biosynthesis pathway of Zanthoxylum armatum. Grafting can affect the expression of ZaGGPPS gene in leaves and stems. The ZaGGPPS gene of Zanthoxylum armatum was cloned and analyzed in order to provide theoretical basis for further study on the molecular mechanism of aroma formation of Zanthoxylum armatum and selection of excellent varieties by molecular biological means.
Hits
Hits
Downloads
Comment