Subjects: Biology >> Botany >> Applied botany submitted time 2021-05-27 Cooperative journals: 《广西植物》
Abstract： Pinus taiwanensis var. damingshanensis is a kind of endemic Alpine pine of Guangxi and Guizhou, which has high economic and ecological value. Its natural population has not been fully protected and utilized for a long time, which is not conducive to long-term stable development of this species. In order to protect and exploit the natural genetic resources of Pinus taiwanensis var. damingshanensis, 12 SSR markers were used to study the genetic diversity of three natural populations of Pinus taiwanensis var. damingshanensis, and to analyze the genetic differentiation and gene flow among populations, so as to provide reference for the protection strategy of this species. The results were as follows: 37 alleles were detected by 12 pairs of primers, and the percentage of polymorphic loci was 100%. For every site, the average number of observed alleles (Na) was 3.08, and the average number of effective alleles (Ne) was 1.68. The number of effective alleles varied greatly among different loci. The average observed heterozygosity (Ho) was 0.35, the average expected heterozygosity (He) was 0.40, and the average polymorphism information content (PIC) was 0.31 for every site. The Shannon's diversity index of the three populations ranged from0.48 to 0.65, and the Nei's gene diversity ranged from0.27 to 0.39. Compared with other related species of pines, the genetic diversity was low. For each population, the average observed heterozygosity was 0.40, and the average expected heterozygosity was 0.33 ， the average number of effective alleles was 1.58. The genetic differentiation coefficient (Gst) among populations was 0.10. Most of the variation existed in the population and the genetic differentiation level among populations was low. The range of gene flow (Nm) was 2.74, indicating that gene exchange between populations was sufficient of Pinus taiwanensis var. damingshanensis. This study can provide an important reference for the protection of biodiversity, and lay a foundation for the scientific utilization of Pinus taiwanensis var. damingshanensis.
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