Subjects: Biology >> Botany >> Applied botany submitted time 2021-04-29 Cooperative journals: 《广西植物》
Abstract： It has been clarified that GeBP transcription factor regulates the growth and development of plant epidermal hair and participates in the control of plant leaf development. The bioinformatics methods were used to identify the GeBP gene family in the whole soybean genome, and from physicochemical properties of amino acids, as well as gene structure, physical distribution of chromosomes, phylogenetic tree, and multiple sequence comparison, the functional domain, tissues expression and other basic characteristics of GmGeBP gene family were analyzed. A total of nine members of GmGeBP transcription factor family were identified, of which only two genes contained introns and all had only one intron, indicating that the gene structure of the family members was relatively simple but stable. The molecular weight of GmGeBPs is 39.65-49.24 kD, and the theoretical isoelectric point is 4.65-9.08; these members are basically acidic amino acids, which are hydrophilic and unstable proteins.The chromosome physical distribution showed that 9 genes were unevenly distributed on 7 chromosomes, 2 GeBP genes on chromosome 10 and 20，respectively, and 1 gene on chromosome 3, 5, 13, 15 and 19 respectively. The phylogenetic analysis showed that GeBP members of soybean and Arabidopsis thaliana were closely related, clustered into four branches respectively, but far away from Oryza sativa. The analysis of domains showed that all the nine GmGeBP members contained DUF573 domain, which was probably the domain interacting with cis-acting elements of target genes in GeBP transcription factors. By analyzing the expression of GmGeBP transcription factor family, we found that the expression of different genes in different tissues is different, with a certain specificity. The analysis and identification of GmGeBP transcription factor gene family provided a theoretical basis for further studying the molecular role of soybean epidermal development.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-04-29 Cooperative journals: 《广西植物》
Abstract： Plant DUR3 homologous protein is a high affinity urea transporter which belongs to the family of sodium/solute symporter family, and plays an important role in the active absorption of exogenous urea and redistribution of endogenous urea by plants. The purpose of this study was to clarify the existence, structure and evolution situation of cotton DUR3 gene. Based on bioinformatics methods, DUR3 genes were identified from Gossypium hirsutum and Gossypium raimondii genomic sequences, and then the gene structure, transmembrane domain, motif location, Three DUR3 genes were identified from the A and D subgroup chromosomes of upland cotton and Gossypium raimondii genomic sequences. These 3 cotton DUR3 homologous proteins, like other plant DUR3 homologous proteins, have 15 transmembrane domains and 3 highly conserved motifs with consistent positions. The gene structure analysis showed that the number of exons of DUR3 genes in dicotyledons was significantly higher than that in monocotyledons, and so were the cotton DUR3 genes. Phylogenetic analysis revealed that the different species DUR3 proteins were classified according to species kinship, and cotton clustered in one branch with dicotyledons. The Ka/Ks values of orthologous and paralogous genes of DUR3 were generally more than 1, indicated that those genes mainly experienced positive selection among evolution. The results of this study will provide a theoretical basis for further research on cotton DUR3 homologous protein.
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