Subjects: Biology >> Botany >> Applied botany submitted time 2023-07-26 Cooperative journals: 《广西植物》
Abstract： Camellia perpetua is the only rare and endangered plant in the genus Camellia that blooms almost all year round. It provides reference for people to know the flower bud differentiation process and the changes of endogenous hormones in leaves at different flower development period and annual growth cycles, as well as clarify the continuous flowering mechanism of this species. In this study, the process of C. perpetua bud differentiation was observed by paraffin sections. The contents of abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellin (GA3) and zeatin nucleoside (ZR) in leaves at different flower development period and in leaves with flower bud and leaves without flower bud in annual growth cycle were determined by enzyme-linked immunosorbent assay (ELISA). The results were as follows: (1) The flower bud differentiation sequence of C. perpetua was from the outside to the inside, and it was divided into 6 period, which lasted 35 days in total, from the bud differentiation to flowering about 2 months. (2) The contents of ABA and GA3 and the ratios of GA3/ABA and (IAA + GA3) /ZR were higher in flower bud morphological differentiation period, while the content of IAA and ZR and the ratios of IAA/ABA and ZR/ABA were lower. (3)The contents of ABA, IAA and ZR in the leaves with flower bud were higher than those in the leaves without flower bud. The IAA/ZR and (IAA + GA3) /ZR ratios were lower than those of the leaves without flower bud. In conclusion, the flower bud differentiation to flowering time of C. perpetua is shorter. High levels of ABA and GA3 and low levels of IAA and ZR are beneficial to flower bud differentiation. Higher levels of ABA, IAA and ZR are beneficial to flower bud development.
Subjects: Biology >> Botany >> Applied botany submitted time 2022-07-05 Cooperative journals: 《广西植物》
Abstract： The investigation of the calcium speciation characteristics in leaf under different habitats can help to understand deeply the adaptation mechanisms of golden Camellia species to soil calcium and provide a reference for the development of their conservation measures. In this study, 10 species of golden Camellia from calcareous soil habitats and 4 from acidic soil habitats were employed. The calcium content (Soil-Ca) and pH (Soil-pH) of habitat soil, and the content of calcium nitrate and calcium hloride (AIC-Ca), water soluble calcium (H2O-Ca), calcium pectate (NaCl-Ca), calcium phosphate and calcium carbonate (HAC-Ca), calcium oxalate (HCl-Ca), sili-cate calcium (Res-Ca), and total calcium (Tot-Ca) of leaves were measured. The results were as follows: (1) the Soil-Ca and Soil-pH of calcareous soils were extremely significantly (P<0.01) higher than those of acidic soils. (2) The leaf calcium speciation of golden Camellia species from calcareous soil habitats was primarily HCl-Ca (41.17%), while species from acidic soil habitats was primarily NaCl-Ca (43.10%), and all calcium speciation and total calcium content in leaves of species from calcareous soil were extremely significantly (P<0.01) higher than that from acidic soil except for AIC-Ca and NaCl-Ca. (3) Correlation analysis showed that most of the content of calcium speciation in leaves was extremely significantly (P<0.01) and positively correlated with Soil-Ca and Soil-pH, indicating that soil environment had an important influence on the leaf calcium speciation characteristics of golden Camellia species. (4) According to ANOVA, the content of each leaf calcium speciation was extremely significant (P<0.01) difference among the species in golden Camellia revealing that the calcium speciation char-acteristics have a considerable variation during the species differentiation. (5) Cluster analysis based on calcium speciation characteristics showed that the 14 species of golden Camellia could be grouped into three categories. In summary, the differences in leaf calcium speciation of plants of golden Camellia species in different habitats may be the consequence of a combination of soil environmental and genetic factors.
Subjects: Biology >> Botany >> Applied botany submitted time 2022-03-30 Cooperative journals: 《广西植物》
Abstract： Keteleeria calcarea is a precious tree species in the mid-subtropical karst area, this species has a narrow distribution range and very few seedlings in wild populations, which severely limits the natural renewal of the population. In order to explore the causes for the endangerment of this species and provide reference for its protection and utilization, the cones and seeds morphological characteris-tics of K. calcarea were measured, and the effects of temperatures, light conditions, soil water con-tents, substrates, storage temperatures and time, and different geographical provenances on seed ger-mination were studied. The results were as follows: (1) The average seed setting rate of K. calcarea cone was 7.45%±6.54%, the seed wing length was 2.27±0.32 cm, the average seed length was 1.55±0.15 cm (excluding seed wing), the average width was 0.62±0.05 cm, the average thickness was 0.46±0.04 cm, and the average 1 000-seed weight was 214.81±14.76 g, the average water content of dried seeds was 15.28%±1.66%. (2) The optimum temperature for seed germination was 25 ℃, and the germination rate decreased significantly at 20 ℃ and 30 ℃ (P<0.05); Seed germination did not need light, but the germination rate under periodic light was significantly higher than that under con-tinuous light and continuous darkness (P<0.05); The seeds could germinate under the conditions of 10%-30% soil water contents, and could withstand a certain degree of drought; Peat soil and perlite with loose texture and good air permeability were suitable for germination; The seeds were not re-sistant to storage and should be sown in time after collection; The seed germination rate of Sanjiang population in Gongcheng County was the highest among the three geographical provenances. The seed setting rate of K. calcarea cone was low, the seed germination was strict with temperature, and the seed was easy to lose vigor, which may be an important reason for its endangerment.
Subjects: Biology >> Botany >> Applied botany submitted time 2020-01-12 Cooperative journals: 《广西植物》
Abstract：采用砂培法，分别以2 种喜钙型金花茶[直脉金花茶（Camellia multipetala）和柠檬金花茶（C. limonia）]和2 种嫌钙型金花茶[金花茶（Camellia nitidissima）和东兴金花茶（C.tunghinensis）]幼苗为试验材料，用不同浓度的Ca2+（设为5、25、50、100 mmol·L-1）营养液进行培养，研究其对两种不同类型金花茶的光合及生理指标的影响，以期探讨喜钙型金花茶对高钙环境的生理适应机制，分析嫌钙型金花茶的避钙机理。结果表明：两种嫌钙型金花茶的净光合速率Pn、气孔导度Gs、实际光化学效率ΦPSII、电子传递效率ETR、Chl a、Chlb、Chl 总量等均随Ca2+浓度的升高而降低，表明高钙环境降低了嫌钙型金花茶的光合能力；喜钙型金花茶的这些指标在不同Ca2+浓度下并无显著变化，高钙环境并未影响其光合作用的正常进行。嫌钙型金花茶叶片丙二醛含量和PSII 最大光化学效率Fv/Fm 在高钙环境下并未有显著变化，表明其光合膜系统还未受到伤害。随着Ca2+浓度的升高，嫌钙型金花茶叶片脯氨酸含量显著增加，而可溶性糖含量变化并不大；喜钙型金花茶在不同Ca2+浓度下脯氨酸和可溶性糖含量的变化均不明显，但其可溶性糖的含量明显高于嫌钙型金花茶。喜钙型金花茶对外界钙浓度的变化并不敏感，其适应高钙的生理机制可能与叶片较高的可溶性糖含量有关；而嫌钙型金花茶对高钙环境的适应性较差，这可能是其不能在钙质土上生长的主要原因。
Hits
Hits
Downloads
Comment