Subjects: Biology >> Botany >> Applied botany submitted time 2020-05-28 Cooperative journals: 《广西植物》
Abstract： Calcium-dependent protein kinases (CDPKs) are important calcium sensors in higher plants, which play an important role in plant resistance to envirenment stress.In this study, a full-length cDNA of RgCDPK gene was cloned from Rehmannia glutinosa. Meanwhile, the bioinformatics analysis was used the online software, quantitative real-time PCR technique was used to detect RgCDPK expression level in different tissues. The results were as follows: (1)The full-length cDNA sequence of RgCDPK gene was 1 770 bp and encoded 589 amino acid residues; (2)Sequence alignments and structural analysis revealed that its protein contained serine/threonine protein kinase region and EF-hand region, which were typical domains of calcium-dependent protein kinase. Phylogenetic analysis showed the highest similarity with Arabidopsis AtCDPK28. Thus, the gene was defined as RgCDPK (Genbank accession No. MT024235); (3)The expression analysis of RgCDPK in different tissues revealed that high transcript levels occurred at leaves. In this study, the CDPK gene of Rehmannia glutinosa was successfully cloned, and the expression of the gene in different tissues was found to be different. The results of this study provide theoretical basis for further study on the function of CDPK in biotic, abiotic stresses and continuous cropping obstacles.
Subjects: Biology >> Botany >> Applied botany submitted time 2019-08-27 Cooperative journals: 《广西植物》
Abstract：为探究夏枯草中GGPPS基因的生物学特性及功能，本实验在夏枯草转录组测序的基础上设计特异性引物，采用逆转录 PCR 技术获得夏枯草中GGPPS基因的全长核苷酸序列，并进行生物信息学分析；采用 qPCR 法分析PvGGPPS基因在不同外源性物质诱导下在夏枯草果穗中的表达量以及该基因在夏枯草不同组织中的表达量。结果表明：PvGGPPS 基因开放阅读框1 092 bp，编码363个氨基酸，理论分子量为38 815.68 D，等电点为5.69。PvGGPPS蛋白具有异戊烯基焦磷酸合酶家族的特征结构域。系统进化树表明PvGGPPS蛋白与丹参、毛喉鞘蕊花GGPPS蛋白具有较高的亲缘关系。qPCR分析表明，PvGGPPS基因在叶中表达量高于果穗及茎。对果穗施加7种外源性物质处理24 h后，GA3 处理组该基因表达量升高。PvGGPPS 基因在夏枯草不同组织中表达量差异较大，且受外源物质诱导表达。该研究结果为进一步研究PvGGPPS 基因对夏枯草萜类成分合成途径中的功能及表达调控奠定基础。
Subjects: Biology >> Botany >> Applied botany submitted time 2019-02-25 Cooperative journals: 《广西植物》
Abstract：在夏枯草转录组测序的基础上设计特异引物，采用逆转录PCR技术获得该基因的全长核苷酸序列，并进行生物信息学分析，采用qRT-PCR法分析PvDXS在夏枯草不同组织及不同外源性物质诱导下的表达量。克隆得到的PvDXS基因开放阅读框2181 bp，编码726个氨基酸，理论分子量为78 040.47 D，等电点为6.75,PvDXS 蛋白具有Transketolase_C 结构域和Transket_pyr 结构域，系统进化树结果表明，PvDXS 蛋白与丹参、长春花的DXS（SmDXS2、CrDXS2）亲缘关系较近，推测PvDXS 属于第II 类DXS 蛋白。qRT-PCR 分析表明，PvDXS 基因在叶中表达量高于果穗及茎。对果穗施加7种外源性物质处理24 h后，GA3处理组该基因表达量升高，其它6种外源性物质处理后表达量均降低，其中CaCl2、SNP、SA处理后该基因的表达量显著降低。PvDXS基因在不同组织中表达量差异较大，且受外源物质诱导表达，该研究结果为进一步研究PvDXR基因对夏枯草萜类成分合成途径中的功能及表达调控奠定基础
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