Subjects: Biology >> Botany >> Applied botany submitted time 2022-08-05 Cooperative journals: 《广西植物》
Abstract： Sea buckthorn (Hippophae rhamnoides) is an important dioecious tree species of protective plantation with insufficiently documented on the sexual responses difference to environmental stress as well as the interaction between sexual competition and heavy metal stress. In order to provide the practical guidance for repairing heavy metal pollution in soil, the physiological responses and tolerance of male and female sea buckthorn plants under Mn stress and different gender interaction patterns were discussed. The physiological responses of sea buckthorn under Mn stress ( 4 000 mg·kg-1 ) and three different gender combinations ( female× female, male×male, female × male) were determined, including the contents of chlorophyll, peroxidase (POD), superoxide dismutase (SOD), malondialdehyde (MDA), total phenols, free proline (Pro), soluble sugar (SS), betaine and manganese in male and female leaves. The results were as follows： (1) Under Mn stress, the SOD of male plants (M/FM) was the highest in all competition combinations, while the MDA content was not significantly increased compared with the controls, indicating that the male plants had better antioxidant capacity and less membrane oxidative damage. (2) M/FM accumulated more proline and showed better osmotic adjustment ability under Mn stress, indicating that male plants under sexual competition showed better tolerance to Mn stress. (3) The study indicated that male-female interaction and Mn stress interaction significantly affected the physiological response characteristics of sea buckthorn leaves, such as chlorophyll content, antioxidant enzyme activity and osmotic adjustment. Principal component analysis showed that the contents of SOD, POD, MDA, chlorophyll b (Chlb), SS and Pro in leaves could be used as the main physiological response indicators. The results can be used as a reference for the remediation of soil heavy metal pollution by sea buckthorn plants.
Subjects: Biology >> Zoology submitted time 2018-12-24 Cooperative journals: 《动物营养学报》
Abstract：本试验旨在研究乳酸链球菌素（Nisin）对腹泻小鼠盲肠菌群结构和脂代谢的影响。选择7～9周龄无特定病原体（SPF）级50只小鼠（雌雄各占1/2），随机分成5组，分别为空白对照组、阴性对照组、环丙沙星组、氨苄青霉素组及Nisin组。除空白对照组小鼠腹腔注射等量灭菌生理盐水外，其余各组小鼠连续3 d腹腔注射致病性大肠杆菌（E.coli）O1悬液（2.50×1011 CFU/mL）0.2 mL/只构建小鼠腹泻模型。连续注射3 d后，空白对照组和阴性对照组灌胃灭菌生理盐水，其他组灌胃对应物质，每天2次，每次0.3 mL，连续灌胃15 d。第15天灌胃2 h后采样，采用高通量测序技术对小鼠盲肠内容物中细菌结构测序分析，通过酶联免疫吸附测定（ELISA）法对小鼠血清、空肠、回肠和脑组织中金属肽酶含血小板反应蛋白1(ADAMTS1)、总胆固醇（TC）和胰岛素(INS)的含量进行检测。结果显示：与阴性对照组相比，Nisin使小鼠血清、回肠和脑组织中ADAMTS1的含量和回肠中INS的含量显著降低（P<0.05），血清、空肠和脑组织中TC的含量显著增加（P<0.05），并使小鼠各阶段的体重显著增加（P<0.05）。Nisin组小鼠盲肠菌群丰富度最高(ACE指数=2 469.54、Chao1指数=3 340.29)且多样性最高(Shannon指数=7.56)，而阴性对照组则最低。Nisin组中疣微菌门(Verrucomicrobia)、蓝细菌门(Cyanobacteria)、拟杆菌门(Bacteroidetes)及厚壁菌门(Firmicutes)为优势菌门。阴性对照组中放线菌门(Actinobacteria)、芽单胞菌门(Gemmatimonadetes)、变形菌门(Proteobacteria)及拟杆菌门为优势菌门。由此得出，Nisin可提高盲肠菌群多样性，降低血清、回肠和脑组织中ADAMTS1的含量和回肠中INS的含量，增加血清、空肠和脑组织中TC的含量，从而影响腹泻小鼠盲肠菌群结构及脂代谢。
Subjects: Biology >> Zoology submitted time 2017-10-11 Cooperative journals: 《动物营养学报》
Abstract：本研究旨在探讨饲粮添加苹果果胶寡糖（apple pectic oligosaccharide,APOS）对断奶大鼠生长性能、抗氧化能力和肠道健康的影响。试验选取24只健康的断奶Wistar大鼠，按照体重相近的原则分为2个处理，分别饲喂基础饲粮和添加800 mg/kg APOS的饲粮，试验期为14 d。结果表明：饲粮添加APOS显著提高了断奶大鼠平均日增重和平均日采食量（P<0.05），显著降低了料重比（P<0.05）；饲粮添加APOS可显著提高断奶大鼠血清总抗氧化能力（P<0.05），降低血清中丙二醛的含量（P<0.05）；饲粮添加APOS还可显著提高空肠黏膜绒毛高度/隐窝深度值（P<0.05）；此外，饲粮添加APOS也提高了盲肠食糜中乳酸菌和双歧杆菌的数量（P<0.05），降低了盲肠食糜中大肠杆菌的数量（P<0.05）。综上所述，在断奶大鼠的饲粮中添加APOS可以提高其生长性能，而这可能源于其改善了大鼠抗氧化能力、空肠黏膜形态和肠道菌群结构。
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