Subjects: Biology >> Botany >> Applied botany submitted time 2022-03-30 Cooperative journals: 《广西植物》
Abstract： Marine plants and their associated microorganisms can produce many secondary metabolites with novel structures and unique activities. The Beibu Gulf is rich in marine plant species resources. According to statistics, there are 43 species of marine plants in 3 phyla. This paper reviews the research progress of the secondary metabolites of marine plants and their co-epiphytic microorganisms in the Beibu Gulf since 2002. 59 new compounds and 35 known active compounds were obtained from 11 species of mangrove plants and 7 species of associated microorganisms, and 3 new compound and 7 known active compounds were obtained from 3 species of seagrass plants. 25 new compounds and 8 known active compounds were obtained from 6 species of algae plants and 1 species of symphytic microorganisms, which mainly involved terpenoids, alkaloids, flavonoids and sterols, most of which had good anti-bacterial, anti-oxidation, anti-tumor, anti-inflammatory and enhancing the body immunity. Further research suggestions are put forward. The review provide reference for further study and utilization of marine plants and their associated microorganisms in Beibu Gulf.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-11-04 Cooperative journals: 《广西植物》
Abstract： Mangrove habitat is rich in microfloras and its secondary metabolites have novel structure, which is an important source for mining new drugs. This study preliminarily evaluated the culturable bacterial diversity of sediments, roots, leaves and hypocotyl of Bruguiera gymnorrhiza and the biological activity of bacterial metabolites, and looked for anti-HBV drug source strains. Pure culture technique and 16S rRNA molecular biology technique were employed to determine the species of bacteria and analyze the species diversity. Using HepG2.2.15 cell line as a model, the anti-HBV activity of bacterial metabolites was tested by MTT and PCR techniques. The secondary metabolites of active bacteria were preliminary analyzed by LC-HRMS. The results were as follows: (1) A total of 59 species of bacteria were obtained, which belonging to 4 phyla, 5 classes, 14 orders, 23 families, and 36 genera, among which Bacillus was the dominant genus. Strains GXIMD07402, GXIMD07665 and GXIMD07384 are potential new species of Pseudooceanicola, Thioclava, and Aestuariibaculum, respectively. (2) The results of anti-HBV activity showed that GXIMD07366, GXIMD07616, GXIMD07384, GXIMD07550 and GXIMD07445X could significantly reduce the level of HBV DNA in the supernatant of HepG2.2.15 cells, and the inhibition rates were 51%, 47%, 63%, 52%, and 47%. (3) Four main secondary metabolites of the highly active strain GXIMD07384 were identified as Adenosine, Cyclo(L-Pro-L-OMet), Acremine G, and 7,8-dimethylbenzo[g]pteridine -2,4(1H,3H)-dione. The results of the study confirm that the species diversity of culturable bacteria in the habitat of Bruguiera gymnorrhiza is rich and contains anti-HBV active strains, which provide a basis for the subsequent application of marine microbial resources.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-06-02 Cooperative journals: 《广西植物》
Abstract： This study aims to explore potential new bacterial species and bioactive substances in mangrove plants, which can enrich the diversity of mangrove microorganisms and provide strain resources for the development of new active products. 22 samples were collected from three true mangrove plants of Kandelia candel, Bruguiera gymnorrhiza, Rhizophora stylosa, and their habitats, in Guangxi coastal area. Then 22 samples were divided according to different parts such as roots, stems, leaves, flowers, fruits and muds. Eight different culture mediums were used to isolate culturable bacteria, and 16S rRNA gene sequences were used to identify bacteria and analyze diversity. For cultured bacteria, the antibacterial and enzyme activities of fermented crude extracts were screened with Kirby-Bauer method and spot planting method, respectively. The results were as follows:（1）Based on sequencing results of 16S rRNA gene, a total of 35 culturable bacteria were isolated, belonging to 28 genera and 23 families. Bacillus accounted for 14.3% of the total bacteria, which was the dominant bacteria. Meanwhile, 11 potential new bacterial species were found. （2）Four strains of bacteria showed antibacterial activities, 16 strains had enzyme activities, and Bacillus was the dominant strain in enzyme activities. The above results showed that Guangxi true mangrove plants were rich in bacterial diversity, and some bacteria showed antibacterial activities and enzyme activities. The new bacterial species and active strain had certain development potential in the new antibiotics and enzymes application.
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