Cloning and expression analysis of sugar transporter gene MeSWEET17b in cassava

Subjects: Biology >> Botany >> Applied botany submitted time 2024-04-17 Cooperative journals: 《广西植物》

XUE Jingjing WEI Zhuowen LUO Xiuqin AN Feifei

Abstract： Cassava (Manihot esculenta) is an important food crop in tropical and subtropical regions. Sugar transporter proteins SWEETs facilitate the flow of sugar between cells and play an important role in plant growth and development. In order to clarify the function of SWEET family genes in cassava, KU50 was used as material in this study, and the gene characteristics of MeSWEET17b were studied by gene cloning, bioinformatics analysis, subcellular localization, in vitro yeast detection and RT-qPCR. The results showed as follows: (1) The open reading frame of MeSWEET17b was 726 bp, encoding 242 amino acids, and located in the plasma membrane. MeSWEET17b had the closest genetic relationship with AtSWEET16 and AtSWEET17, containing 7 transmembrane domains, and belonging to hydrophobic protein. (2) MeSWEET17b mainly transport fructose. (3) The results of RT-qPCR showed that the expression profile of MeSWEET17b in stem was basically consistent with in petiole, and the expression level was the highest at maturity. The expression level of MeSWEET17b was relatively low in leaves, and highly expressed in the expansion stage of tuberous root, while decreased rapidly with the growth of tuberous roots. (4) The KU50 seedlings were subjected to abiotic stress such as high salt (8 g·L-1 NaCl), drought (100 mmol·L-1 mannitol), oxidation (10 % H2O2) and cold (15 ℃ for 24 h, then dropped to 4 ℃ for 24 h). RT-qPCR showed that the expression of MeSWEET17b in leaves and stems had the greatest difference under drought stress. The expression of MeSWEET17b in leaves and fibrous roots changed most significantly under salt stress. However, under oxidation and cold stress, the expression of MeSWEET17b in fibrous root showed a leap increase, while the expression in petiole increased significantly with treatment time. This study provides a reliable reference for further studying the molecular mechanism of sugar transporter SWEETs gene in cassava.

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