Subjects: Biology >> Botany >> Applied botany submitted time 2023-06-05 Cooperative journals: 《广西植物》
Abstract： Pitaya (Hylocereus undatus) is a typical tropical cash crop that is not chilling-tolerant. Low temperature stress seriously affects the quality and yield of pitaya. It is an efficient and safe method to apply exogenous natural substances to improve the chilling tolerance of plants. Salicylic acid (SA) not only regulates many physiological and biochemical processes such as seed germination, growth, fruiting and senescence, but also helps plants to cope with biotic and abiotic stresses. However, the role of exogenous SA in chilling tolerance of pitaya has not been reported. To explore the effect of exogenous SA on the morphology structure and physiology of pitaya seedlings under low temperature, the leaves of "Zihonglong" seedlings were sprayed with SA at four concentrations (0.1, 0.3, 0.5 and 0.7 mmol·L-1). 48 h after spraying, seedlings were incubated at 4 °C. The change in the morphology and leaf tissue structure of pitaya seedlings were observed at 0, 3, 6, and 9 d. Physiological indexes such as relative electrical conductivity, content of malondialdehyde, content of osmotic regulators (soluble sugar, soluble protein, proline) and antioxidant enzymes (superoxide dismutase, peroxidase, catalase, glutathione S-transferase) activity in leaves were measured. The results were as follows: (1) Under low temperature stress, pitaya seedlings showed chilling injury of grade 0, gradeⅠand gradeⅡ. The leaf shape, color and cell structure of pitaya with different chilling injury symptoms were quite different. The percentages of grade Ⅰand gradeⅡchilling injury of pitaya seedlings treated with SA were significantly reduced. (2) Compared with the low temperature control, SA treatment could reduce the relative conductivity and malondialdehyde content of pitaya leaves, and increase the content of osmotic regulators and antioxidant enzyme activities.(3) After comparing with different SA treatments, SA at 0.3 mmol·L-1 had the best effect on relieving chilling injury symptoms, reducing the relative conductivity and malondialdehyde content, increasing the soluble sugar and proline content, and increasing the activities of superoxide dismutase and glutathione S-transferase. The optimal SA concentrations for increasing soluble protein content, peroxidase activity and catalase activity were 0.7, 0.1 and 0.5 mmol·L-1, respectively. In summary, exogenous SA could alleviate chilling injury of pitaya seedlings under 4 °C, and had a positive physiological effect on improving chilling tolerance of seedlings. Exogenous SA at 0.3 mmol·L-1 was more suitable.
Subjects: Biology >> Zoology submitted time 2018-12-24 Cooperative journals: 《动物营养学报》
Abstract：牛磺酸（Tau）是一种存在于动物体内的游离氨基酸，具有抗氧化、增强免疫力及抗细胞凋亡等作用。作为饲料添加剂，牛磺酸在畜牧生产中的应用逐渐受到关注，具有提高生产性能、繁殖性能和降低应激损伤等作用。本文阐述了牛磺酸的生理功能及其作用机制，并综述了近年来牛磺酸在猪生产中的应用效果，以期为牛磺酸在猪生产中更好地应用提供参考。
Subjects: Biology >> Bioengineering submitted time 2018-05-16 Cooperative journals: 《中国生物工程杂志》
Abstract：目的借助实验室前期质谱分析技术和数据分析研究基础，采用免疫共沉淀(Co-IP)和蛋白沉降实验(GST pull-down)验证HBV X蛋白与Tab1蛋白的相互作用，为进一步研究HBx在HBV慢性感染致癌机制中的作用提供一定的实验依据。方法成功构建pGEX-2TK-GST-HBx质粒，对GST-HBx融合蛋白进行诱导表达，与GST-beads结合孵育，构建pcDNA3.1/myc-His(-)B-Tab1，转染293T细胞使其表达，然后GST pull-down体外试验验证二者的相互作用；构建pcDNA3.1/myc-His(-)B-Tab1和pcDNA3.1-3×flag-HBx真核表达质粒, 共转染293T和HepG2细胞使其表达，通过Co-IP实验验证抗Myc抗体可以将HBx从细胞裂解液中沉淀下来，证实了它们在两种细胞系中存在相互作用。 结果显示了HBx和Tab1在体内外条件下能够发生相互作用，为进一步明确HBV X蛋白功能及作用机制奠定基础。
Subjects: Biology >> Zoology submitted time 2017-10-23 Cooperative journals: 《动物营养学报》
Abstract：随着养猪业集约化、工厂化的发展，为了方便管理和降低成本，实际生产中常常扩大饲养密度，导致猪生存空间和食物等不足，诱发猪心理和行为方式等的改变。此外，高饲养密度加大了猪舍内环境负担，使舍内有害气体含量增多，增加了呼吸道和消化道疾病发生的概率，严重影响着猪群的健康并制约猪生长性能的发挥。本文就饲养密度对猪群健康和猪舍环境的影响做一论述。
Subjects: Biology >> Bioengineering submitted time 2017-07-24 Cooperative journals: 《中国生物工程杂志》
Abstract：旨在建立一种可同时检测猪、牛、羊、鸡、鸭、鹅六种动物源性成分的六重荧光定量PCR方法。根据猪、牛、羊、鸡、鸭、鹅细胞核基因组保守序列，参照序列比对结果选择差异位点设计6对特异性引物和6条以不同荧光素标记的Taqman 探针。通过对PCR 反应体系和反应条件的优化筛选，建立能同步定量检测猪、牛、羊、鸡、鸭、鹅源性成分的六重实时荧光定量PCR方法。应用此方法分别对18种不同源性动物DNA和200 份不同来源样品进行猪牛羊鸡鸭鹅源性成分检测，结果表明，所建立的六重荧光定量PCR方法灵敏度高，对六种动物的最低核酸检测量分别为0.049ng、0.048ng、0.085ng、0.13ng、0.162ng、0.074ng（50μl体系）；特异性强，对狗、兔、鼠、驴、马、骆驼等其他12种动物无特异性扩增；200份样品的检测结果表明六重qPCR检测方法敏感，同一反应体系下实现一次对6种动物快速定量检测，耗时短、效率高、适用性广，可用于肉品、奶品、皮毛和饲料等动物产品猪牛羊鸡鸭鹅源性成分单一或混合物种的鉴别诊断。
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