Subjects: Biology >> Botany >> Applied botany submitted time 2022-03-30 Cooperative journals: 《广西植物》
Abstract： Marine plants and their associated microorganisms can produce many secondary metabolites with novel structures and unique activities. The Beibu Gulf is rich in marine plant species resources. According to statistics, there are 43 species of marine plants in 3 phyla. This paper reviews the research progress of the secondary metabolites of marine plants and their co-epiphytic microorganisms in the Beibu Gulf since 2002. 59 new compounds and 35 known active compounds were obtained from 11 species of mangrove plants and 7 species of associated microorganisms, and 3 new compound and 7 known active compounds were obtained from 3 species of seagrass plants. 25 new compounds and 8 known active compounds were obtained from 6 species of algae plants and 1 species of symphytic microorganisms, which mainly involved terpenoids, alkaloids, flavonoids and sterols, most of which had good anti-bacterial, anti-oxidation, anti-tumor, anti-inflammatory and enhancing the body immunity. Further research suggestions are put forward. The review provide reference for further study and utilization of marine plants and their associated microorganisms in Beibu Gulf.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-11-04 Cooperative journals: 《广西植物》
Abstract： Mangrove habitat is rich in microfloras and its secondary metabolites have novel structure, which is an important source for mining new drugs. This study preliminarily evaluated the culturable bacterial diversity of sediments, roots, leaves and hypocotyl of Bruguiera gymnorrhiza and the biological activity of bacterial metabolites, and looked for anti-HBV drug source strains. Pure culture technique and 16S rRNA molecular biology technique were employed to determine the species of bacteria and analyze the species diversity. Using HepG2.2.15 cell line as a model, the anti-HBV activity of bacterial metabolites was tested by MTT and PCR techniques. The secondary metabolites of active bacteria were preliminary analyzed by LC-HRMS. The results were as follows: (1) A total of 59 species of bacteria were obtained, which belonging to 4 phyla, 5 classes, 14 orders, 23 families, and 36 genera, among which Bacillus was the dominant genus. Strains GXIMD07402, GXIMD07665 and GXIMD07384 are potential new species of Pseudooceanicola, Thioclava, and Aestuariibaculum, respectively. (2) The results of anti-HBV activity showed that GXIMD07366, GXIMD07616, GXIMD07384, GXIMD07550 and GXIMD07445X could significantly reduce the level of HBV DNA in the supernatant of HepG2.2.15 cells, and the inhibition rates were 51%, 47%, 63%, 52%, and 47%. (3) Four main secondary metabolites of the highly active strain GXIMD07384 were identified as Adenosine, Cyclo(L-Pro-L-OMet), Acremine G, and 7,8-dimethylbenzo[g]pteridine -2,4(1H,3H)-dione. The results of the study confirm that the species diversity of culturable bacteria in the habitat of Bruguiera gymnorrhiza is rich and contains anti-HBV active strains, which provide a basis for the subsequent application of marine microbial resources.
Subjects: Biology >> Botany >> Applied botany submitted time 2019-03-14 Cooperative journals: 《广西植物》
Abstract：设计9种分离培养基，采用稀释涂布法从14份真红树植物的46份组织样品中分离纯化内生细菌。基于菌株形态学特征和16S rRNA基因序列确定分离菌株的种属，并分析其物种多样性，采用秀丽隐杆线虫模型筛选菌株延缓衰老活性。结果表明：通过基因序列去重复后从46份真红树植物组织样品中获得32株海洋细菌，基于菌株16S rRNA基因序列信息分析，覆盖12科17属，其中芽孢杆菌属（Bacillus）为优势菌属，并获得1株疑似橙单胞菌属（Aurantimonas）新种，16S rRNA基因序列相似性低于97%。经过秀丽隐杆线虫粗筛发现3株海洋细菌具有显著延缓线虫衰老的活性（P<0.05）。研究结果表明海南西海岸真红树内生细菌具有物种多样性，部分菌株具有延缓线虫衰老活性。
Subjects: Biology >> Botany >> Applied botany submitted time 2018-10-26 Cooperative journals: 《广西植物》
Abstract：无瓣海桑果实为真红树无瓣海桑的果实。研究无瓣海桑果实不同提取物对 D-半乳糖所致衰老小鼠学习记忆能力影响及其作用机制。采用 Morris 水迷宫实验测量无瓣海桑果实不同提取物对小鼠的学习记忆能力影响，HE 染色观察各组小鼠脑部神经细胞的变化情况，并测定脑组织超氧化物歧化酶（SOD）活力、谷胱甘肽过氧化物酶（GSH-Px）活力、一氧化氮（NO）含量和单胺氧化酶（MAO）活力。结果表明与模型组相比，无瓣海桑果实不同提取物处理组小鼠在水迷宫实验中逃避潜伏期明显缩短（P＜0.05），目标象限停留时间明显增加（P＜0.05）。无瓣海桑果实不同提取物处理组小鼠脑部神经细胞损伤与模型组相比明显减少，小鼠脑部 SOD 酶活力和 GSH-Px 酶活力提高（P＜0.05），NO 含量和 MAO 活力在脑部显著降低（P＜0.05）。无瓣海桑果实不同提取物对 D-半乳糖致衰老小鼠学习记忆能力有改善作用，无瓣海桑果实不同提取物通过提高小鼠脑内源抗氧化酶（SOD、GSH-Px）活力，降低脑部 NO 含量和 MAO 活力来提高 D-半乳糖致衰老小鼠的学习记忆能力。
Subjects: Biology >> Botany >> Applied botany submitted time 2018-09-07 Cooperative journals: 《广西植物》
Abstract： The 95% ethanol extracts of the leaves of Rhizophora stylosa, stems and leaves of Kandelia candel, leaves of Pongamia pinnata and stems of Aegiceras corniculatum were extracted by ultrasonic extraction method. The cytotoxicity of 95% ethanol extracts from these 4 mangrove plants on prostate cancer cells DU145 and PC3 was tested by MTS assay. The results showed that the anti-proliferation on prostate cancer cell DU145 of 95% ethanol extract of A. corniculatum stems was the strongest among the tested samples with IC50 value of 75.23, 88.81 and 76.53 μg·mL-1 to DU145, respectively. On this basis, the ethanol extract of A. corniculatum stems was successively treated with petroleum ether, ethyl acetate and n-butanol to yield 4 fractions , then the ethyl acetate fraction of A. corniculatum stems (EACS) was tested for anticancer activities against 19 tumor cells (HT-29, SW480, DLD-1, COLO205, PC3, DU145, SKOV3-S, A2780, SGC-7901, Tca-8113, MDA-MB-231, HepG2, SMMC-7721, Bel-7402, MHCC-97H, Hela, PANC-1, EJ, A549) and antiproliferative effects of 3 normal cells (HUVEC, EC304, RWPE-1) by MTS assay, the effect of EACS on proliferation of HT-29, DLD-1, SW480, DU145, PC3 and SKOV3-S cells was detected by the colony formation assay. The results showed that EACS exhibited different degrees of inhibitory effect on proliferation of 16 tumor cells and 3 normal cells, and had the stronger inhibitory effect on the proliferation of RWPE-1 with IC50 value of 22.78 μg·mL-1 after 72 hours of drug treatment . EACS inhibited the colony formation of HT-29, DLD-1, SW480, DU145, PC3 and SKOV3-S cells in a dose-dependent manner. The results provided a scientific reference for the further development and utilization of the above 4 mangrove plants. At the same time, it was initially determined that A. corniculatum has significant anti-tumor activity and is expected to be further developed as a new anti-tumor drug.
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