Subjects: Biology >> Botany >> Applied botany submitted time 2024-03-26 Cooperative journals: 《广西植物》
Abstract： To promote the restoration and reconstruction of ecosystem in the water-land ecotone, based on typical sample investigation, the variation patterns of vegetation species composition and diversity, and soil nutrients under different vegetation types were studied using Pearson correlation coefficient method and redundancy analysis method. The results were as follows: (1) There were significant differences in plant community structure and species diversity of different vegetation types (gravel zone, grass zone, shrub-grass zone, trees zone). As the submersed duration decreased, the water-land ecotone gradually evolved from scattered herbaceous plant communities to grass, shrub, and tree plant communities, and the α diversity of vegetation species (Shannon-Wiener index, Pielou index and Simpson index ) and vegetation coverage showed a gradually increasing trend, which were lowest value on the gravel zone and the highest on the trees zone. (2) There were significant differences in soil nutrient content among different vegetation types. With the decreased of submersed duration, soil organic matter content gradually increased, while soil water content, available nitrogen, available phosphorus, and available potassium showed a trend of first increasing and then decreasing, the maximum values of these nutrients mostly occurred in shrub-grass zone or trees zone, followed by grass zone, and gravel zone was the lowest. (3) Correlation and redundancy analysis showed that soil available nitrogen, available potassium, available phosphorus and organic matter were significantly positively correlated with various indicators of vegetation species α diversity, among which soil available nitrogen and available potassium had the strongest correlation with vegetation species diversity. In conclusion: different vegetation types in the Lijiang water-land ecotone have heterogeneous patterns of vegetation species composition and diversity as well as soil nutrients. Moderate submergence is beneficial for vegetation community aggregation and promoting soil nutrient accumulation. Herbaceous plants have stronger adaptability to moderate submergence environments. During the ecological restoration process of the Lijiang water-land ecotone, it is necessary to design restoration plans for different vegetation types and fully consider the relationship between vegetation species diversity and soil available nutrients.
Subjects: Biology >> Botany >> Applied botany submitted time 2022-08-05 Cooperative journals: 《广西植物》
Abstract： Excentrodendron tonkinensis is a constructive species of karst seasonal rainforest and an obligate karst plant, which is also one of the national secondary key protected wild plant and an IUCN endangered plant, with high economic and ecological value. However, there is still a lack of understanding of how the potential suitable areas of Excentrodendron tonkinensis change in the context of global change and its key driving factors, which affect the scientific protection and utilization of Excentrodendron tonkinensis.To assess the impacts of climate change on the extents of the habitat of Excentrodendron tonkinensis, we used the Maximum-entropy model to analyze the potential changes in the geographical distribution in China of future climate scenarios SSP1-2.6 and SSP5-8.5), and tested the influence of the karst geological background distribution on predicting the suitable areas of karst obligate plants. The results show that: (1) in the case of adding karst geological background data, the average AUC of the prediction model for the suitable area is 0.997, which has a good prediction effect. And the model prediction results are strictly limited to the karst region, consistent with the characteristics of Excentrodendron tonkinensis which is karst obligate plant; (2) According to the fitting results of the model, the karst geological background, precipitation of warmest quarter(800-950 mm), and the min temperature of coldest month(7-11 ℃) are the key factors limiting the distribution of Excentrodendron tonkinensis; (3) with the increase of temperature in the future, the area of potential suitable areas for Excentrodendron tonkinensis would expand to higher latitudes karst areas. Large numbers of areas of stable habitats exist in parts of southwest Guangxi and southeastern Yunnan. These results suggest that the karst geological distribution is essential as predicting the potential geographic distribution of karst obligate plants such as Excentrodendron tonkinensis; if the temperature continues to rise in the future, its potential suitable areas will expand to high latitudes, and the degree of endangerment may be affected by climate, which means it is not obvious under the influence of climate changes; parts of southwestern Guangxi and southeastern Yunnan are suitable areas for the conservation and utilization of Excentrodendron tonkinensis under the climate changes in the future.
Subjects: Biology >> Botany >> Applied botany submitted time 2022-06-29 Cooperative journals: 《广西植物》
Abstract： In order to study the adaptive strategy of dominant tree species in a karst seasonal rain forest to heterogeneous habitat conditions, seven dominant tree species in a virgin forest in Nonggang, China, were taken as the research objects. The leaf micromorphological indexes of adult individuals of each tree species and the photosynthetic indexes of leaves in the growing season of these trees were measured. The ecological adaptability of these leaf structural and functional indexes to the habitat gradient in the karst peak cluster depression landform was tested. The results were as follows: (1) Along the karst habitat gradient from depression to peak, nine leaf micro-morphological indexes, such as leaf compactness, palisade tissue thickness, cuticle thickness, and upper epidermis thickness, showed a significant upward trend, while leaf looseness showed a significant downward trend. (2) Along the karst habitat gradient from depression to peak, the light compensation point, dark respiration efficiency, and maximum transpiration rate of these leaves all showed a significant upward trend. However, the maximum water use efficiency, the maximum intercellular CO2 concentration, and the apparent quantum efficiency showed a significant downward trend. (3) Leaf compactness was positively correlated with photosynthetic and transpiration ability indexes. Leaf looseness was negatively correlated with these two types of indexes. The above results show that there is a trade-off relationship between photosynthetic efficiency and drought tolerance in leaf functional traits of dominant tree species in the karst seasonal rain forest. Trees distributed in the depressions have obvious adaptive characteristics to weak light. Trees distributed on the middle slopes show extensive habitat adaptability. Due to the limitation of strong light, high temperature and exposed rock, the trees distributed on the mountain peaks show strong drought adaptability and conservative ecological adaptive strategies.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-12-19 Cooperative journals: 《广西植物》
Abstract： Different varieties have different resistances. In order to further explore the resistance differences in different varieties of pitaya fruit and provide a reference for further study on breeding of pitaya fruit resistance. Our study using Illumina HiSeq 2000 sequencing platform to sequencing the transcriptome of ‘Putongbairou’ （BR） and ‘Ecuador Yellow’ （EY）. Functional classification and enrichment analysis of differentially expressed genes （DEGs） were performed by reference to GO Ontology, KEGG and others databases. The results were as follows: (1) There were 14 248 DEGs between BR and EY, of which 5 446 genes were up-regulated and 8 802 genes were down-regulated. (2) GO functional analysis showed that these DEGs are mainly involved in enzyme catalytic activity, cell components, metabolic processes, etc. Among them, there were 349 differential genes involved in oxidoreductase activity. (3) KEGG pathway analysis showed that most of the DEGs were enriched in metabolism, biosynthesis, etc. , and 12 key genes such as CYP86 and CER1 involving in Cutin, suberine and wax biosynthesis. We found the expression of DEGs involved in oxidoreductase activity were higher in BR than that in EY, which significantly enriched, indicating that may be differences in growth and cell metabolism between BR and EY. DEGs involved in the biosynthesis of Cutin, suberine and wax biosynthesis were up-regulated in BR. Such genes have higher expression in BR, and significantly enriched, suggesting that BR may be superior drought and disease resistance than EY.
Subjects: Biology >> Bioengineering submitted time 2019-01-17 Cooperative journals: 《中国生物工程杂志》
Abstract：目的：基于GC-FID法，对狂犬病疫苗生产过程中采用的β-丙内酯灭活剂进行了含量及稳定性研究。方法：气相色谱条件：采用Agilent DB-624（30 m×0.530 mm×3.00 μm）毛细管柱；升温程序：初始温度为80 ℃，保持1 min，以20 ℃/min的速率升温至200 ℃，保持3 min；色谱柱流量：3 mL/min；检测器温度：250 ℃；进样口温度：150 ℃；载气：氮气，线速度：25 cm/sec；进样量：1 μL，分流比为2:1，进样方式：手动进样。结果：以乙腈作为稀释剂，BPL在1:100～1:32000范围内线性关系良好（R2≥0.999）。在1:200、1:1000、1:8000 三个浓度水平下，加标回收率在95.04%～116.86%之间，相对标准偏差（RSD）为2.6%～3.2%，检测限为0.112 μg/mL。结论：该方法简便、专属性强、稳定且在室温条件下操作，大大降低了对试验条件和技术操作的要求，能够满足灭活狂犬病病毒工艺中对BPL检测的需求。
Subjects: Biology >> Bioengineering submitted time 2018-07-15 Cooperative journals: 《中国生物工程杂志》
Abstract：星形胶质细胞上调基因-1（astrocyte elevated gene-1，AEG-1）在多种肿瘤中过表达，参与肿瘤的形成、转移等过程。本实验利用CRISPR/Cas9技术敲除AEG-1基因并研究其在胶质瘤细胞转移过程中的作用。首先设计构建sgRNA/Cas9二合一表达载体并转染到人胶质瘤U251细胞中，通过TA克隆测序鉴定sgRNA的活性；然后筛选建立稳定的AEG-1敲除U251细胞系，并利用Western blot实验检测AEG-1的敲除效率；最后利用Transwell小室、划痕实验评价AEG-1敲除后对肿瘤细胞迁移能力的影响。结果显示，成功构建靶向敲除AEG-1基因的sgRNA/Cas9二合一表达载体，所构建的载体与实验设计相一致，通过TA克隆测序鉴定sgRNA有活性；成功建立稳定的AEG-1敲除U251细胞系，Western blot实验结果表明敲除效率高达98％；Transwell小室实验、划痕实验结果表明AEG-1敲除U251细胞系的转移能力明显降低。
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