Subjects: Biology >> Botany >> Applied botany submitted time 2023-06-05 Cooperative journals: 《广西植物》
Abstract： To investigate the effects of two different water sources, domestic sewage and nutrient solution, on the decomposition of residual roots and the transformation of chromium chemical forms in Cr6+ contaminated constructed wetlands, a micro Coix lachryma-jobi L constructed wetland was constructed to treat chromium containing wastewater [ using domestic sewage (DWS) and 1/2 Hoagland nutrient solution (HNS) as simulated solutions containing 0, 20, and 40mg· L-1Cr (VI), respectively]. By using the buried root decomposition method, we studied the decomposition dynamics of residual roots and the transformation pattern of chromium chemical forms.The results were as follows : (1) Under 20 and 40 mg· L-1 (Ⅵ) stress, the growth of Coix lacryma-jobi L was inhibited. The plant height and stem diameter of HNS treatment were greater than those of DWS treatment, but the plant height and stem diameter of HNS treatment were more inhibited by Cr (Ⅵ) than those of DWS treatment; (2) The decomposition rate of Coix lacryma-jobi L residual roots decreased with the increase of Cr (Ⅵ) concentration, and the decomposition rate of HNS treatment was higher than that of DWS treatment. After 60 days of decomposition, under DWS treatment, the residual root chromium content of 20 and 40 mg· L-1Cr (Ⅵ) treatment decreased by 11.7% and 8.09% respectively compared with that at the initial stage of root burial, while under HNS treatment, the residual root chromium content decreased by 15.8% and 18.42% respectively. The percentage of residual chromium in Coix lacryma-jobi L root residues at 20 and 40 mg· L-1 treatments decreased with the extension of root burial time, while the percentage of ethanol-extracted chromium and deionized water extracted chromium increased, and the percentage of acetic acid bound chromium increased significantly; (3) In the early stage of residual root decomposition, the COD, TN, NH4-N, and total chromium content in the effluent treated with HNS and DWS increased, and then decreased. The trend of change is consistent with the residual root decomposition process. HNS treated constructed wetlands are more efficient in removing chromium from wastewater. The research results indicate that after harvesting plants in constructed wetlands, root decomposition can quickly increase the chromium content in the effluent, and appropriately improve the nutritional status in wastewater, which can promote residual root decomposition and removal of chromium by wetlands.
Subjects: Biology >> Botany >> Botany research, experiment, plant evolution & development submitted time 2018-06-25 Cooperative journals: 《广西植物》
Abstract： In order to obtain high vitality and regeneration ability of sugarcane protoplast, this experiment was done to study the protoplast of frozen storage liquid combination, frozen storage temperature, frozen storage time and recovery temperature. The results showed that: （1） Different cryopreservation, different cryopreservation temperature and different sources of protoplast of different materials have significant difference on the vitality of the protoplast of sugarcane. Compared with the combination of three frozen liquid deposits, in combination 2 (70% medium +20% serum +10% DMSO), the recovery activity was the strongest after 30 days, as high as 72%. Cryopreserved recovery within 90 days, liquid nitrogen -80 oC and -196 oC freezer, sugarcane protoplast energy difference was not significant, and the vigor was above 75%. But after 90 days of frozen-storage, the protoplast dynamic at -196 oC was stronger than -80 oC after recovery. For different materials, the protoplast dynamic of the young leaves frozen stored for 30 days was up to 79.2%, and the protoplast dynamic of stem tip frozen for 30 days was only 42.7%.
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