Subjects: Biology >> Botany >> Applied botany submitted time 2018-07-23 Cooperative journals: 《广西植物》
Abstract： In order to establish a high-frequency regeneration system for Lycium barbarum. The leaf of Lycium barbarum ‘Ningqi 8’ was selected as materials, to explore the crucial effects of hormone combinations and additives on the induction of somatic embryogenesis, germination and regeneration. The results showed that By orthogonal analysis of 6-BA, 2,4-D and IAA, the optimal hormone combination of ‘Ningqi 8’ somatic embryos was 6-BA 1.0 mg･L-1+2,4-D 0.3 mg･L-1+IAA 0.4 mg･L-1, the highest induction rate could get to 88.67%. Range analysis showed that 6-BA had the most significant effect on somatic embryogenesis. Using suitable proportion of hormone combination was possible to induce somatic embryogenesis of ‘Ningqi 8’, which had a large number of normal morphology. In the culture of somatic embryos, the high concentration of 6-BA was easy to cause vitrification, which was not conducive to the growth and proliferation of ‘Ningqi 8’ somatic embryos. The proliferation rate of somatic embryos increased with the increase of hormone concentration, but the vitrification rate increased too. Then the most efficient proliferation medium for ‘Ningqi 8’ was 6-BA 0.4 mg･L-1+NAA 0.6 mg･L-1, and the highest germination rate of ‘Ningqi 8’ somatic embryo was obtained when IBA 0.3 mg･L-1+GA3 0.4 mg･L-1+ sucrose 10g･L-1 was added. The highest germination rate could get to 89.17%, and the degree of influence on the germination of ‘Ningqi 8’ was IBA > sucrose > GA3. Under the condition of adding GA3 and low concentration sucrose could promote the mature somatic embryos germination. Activated carbon could effectively improve the regeneration rate of ‘Ningqi 8’ somatic embryo, and simultaneously promote the development of the root of germination embryo. The IBA 0.1 mg･L-1+KT 0.4 mg･L-1+ activated carbon 1g･L-1 was found to be suitable medium for regeneration in which ‘Ningqi 8’ demonstrated a highest multiplying capacity, and the regeneration rate could get to 91.67%. The research provides a new avenue for the improvement of Lycium barbarum varieties and breeding of new varieties.
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