Subjects: Biology >> Botany >> Applied botany submitted time 2019-08-26 Cooperative journals: 《广西植物》
Abstract： In order to clear genetic background of different provenance Pinus armandii colnes from seed orchard in Zixishan Mountain of Chuxiong City, Yunnan Province, the needles of 60 clones were taken from six provenances which was used to extract total DNA by modified CTAB method, and the genetic diversity was analyzed by SRAP molecular markers. The results showed that 15 pairs of polymorphic SRAP primers were screened from 100 pairs of primer combinations. After SRAP-PCR were amplified, a total of 194 loci were obtained, and the percentage of polymorphic loci PPB=85.05%, Nei's gene diversity index H=0.233 7, Shannon's information index I=0.341 9, genetic differentiation coefficient between population Gst=0.355 5. The genetic diversity of 6 provenances of P. armandii was high, and the genetic variation was mainly existed within provenance of P. armandii. The genetic distance between the Huize (HZ) provenance and the Weishan (WS) provenance was the closest (D=0.050 1), that the Huize (HZ) provenance and Yiliang (YL) provenance was the farthest (D =0.361 8). The cluster analysis showed that six P. armandii provenances there were into three categories: Huize (HZ) provenance and Weishan (WS) provenance were in one category; Chuxiong (CX), Nanhua (NH) and Yiliang (YL) provenance were in the second category; Tengchong (TC) provenance is a separate category. The results indicated that genetic differentiation in P. armandii clones from seed orchard in Zixishan Mountain of Chuxiong City had a high level, and it could provide theoretical basis and practical guidance for the parents selection during the cross breeding and evaluation of germplasm resources of P. armandii as well.
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