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  • Stoichiometric characteristics of understory plant leaves and soil of three forest types in Maoershan

    Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》

    Abstract: Exploring the stoichiometric characteristics of understory plants’ leaves and soil in different forest types in Maoershan can reveal the adaptation strategies of understory plants in Maoershan, and provide data support for forest management. In this paper, coniferous and broad-leaved mixed forest (ZK), evergreen broad-leaved secondary forest (CLC) and evergreen broad-leaved forest (CL) in Maoershan were selected as three forest types, the leaf stoichiometry of main plants in herb layer and shrub layer, and the soil stoichiometry under three forest types were measured and analyzed. The results were as follows: (1) There was no significant difference in leaf C and N contents between herb layer and shrub layer, but P and K contents in herb layer were extremely significantly higher than that in shrub layer, and N∶P was significantly lower than that in shrub layer. Plants in herb layer was more likely to be restricted by N, plants in shrub layer was more likely to be restricted by P and the utilization efficiency of N and P were higher. There was no significant difference in leaf stoichiometry of plants in shrub layer among different forest types, but there were significant differences in leaf N content, C∶N, C∶P of plants in herb layer among different forest types. Plants in herb layer of coniferous and broad-leaved mixed forest had higher nutrient use efficiency. (2) The soil C and N contents of the three forest types showed that CL > CLC > ZK, and there were extremely significant differences among the three forest types. The soil P content of coniferous and broad-leaved mixed forest was the highest, while that of C∶P and N ∶ P was the lowest. (3) Soil in coniferous and broad-leaved mixed forest significantly affected some leaf stoichiometry of plants in herb layer and shrub layer, while the other two forest types had no significant effect on underforest plants. To sum up, there are significant or extremely significant differences in soil stoichiometry among different forest types in Maoershan. The nutrient requirements and environmental adaptation strategies of plants in herb layer and shrub layer are different. The soil of coniferous and broad-leaved mixed forest has a strong influence on the leaf stoichiometry of understory plants, and the soil with low decomposition efficiency of organic matter in this forest type, and the soil is limited by N due to the low decomposition efficiency of organic matter, so the management of N in the mixed forest should be strengthened.

  • 茎花山柚叶的化学成分研究

    Subjects: Biology >> Botany >> Applied botany submitted time 2022-05-21 Cooperative journals: 《广西植物》

    Abstract: In order to study the chemical constituents in the leaves of Champereia manillana var. longistaminea, the chemical composition was analyzed by the silica gel column chromatography (CC), thin-layer chromatography (TLC), dextran gel column chromatography (Sephadex LH-20), reversed-phase silica (RP-18) gel column chromatography and high performance liquid chromatography (HPLC). The ethyl acetate extraction part of the ethanol extraction was separated and 6 monomer compounds were obtained. The structures of these compounds were identified using nuclear magnetic resonance spectroscopy (NMR), high resolution mass spectrometry (HR-ESI-MS) and the data of the literature. Six compounds were elucidated as Taraxerol (1), 1H-indole-3-carboxylic acid (2), (24R)-cycloartane-3β,24,25-triol (3), (24R,S)-3β-24,31-Epoxy-24-methylcycloartane(4),1-O-linolenoyl-3-O-β-D-galactospyranosyl-snglycerol (5), and Hyloglyceride (6). All compounds 1-6 were isolated from this plant for the first time.

  • 重金属镍胁迫对向日葵幼苗生理生化特性的影响

    Subjects: Biology >> Botany >> Applied botany submitted time 2019-03-14 Cooperative journals: 《广西植物》

    Abstract:试验以向日葵幼苗为试材,采用营养液培养法探索重金属镍胁迫对其生理生化指标的影响,揭示向日葵对镍胁迫的响应机制和幼苗生长发育的影响研究,为镍污染环境修复提供一定的科学依据。结果显示:低浓度的镍胁迫(≤10 mg•L-1)有利于幼苗的生长,高浓度的镍胁迫(≥50 mg•L-1)对幼苗具有明显的抑制作用。随着镍胁迫浓度逐渐增加,向日葵幼苗各项生理生化指标的变化较大,其中叶绿素、可溶性糖(SS)、可溶性蛋白(SP)、过氧化物酶(POD)活性、游离脯氨酸(Pro)的含量均在1~10 mg•L-1呈先上升趋势,50~100 mg•L-1呈下降的趋势;丙二醛(MDA)含量则呈现持续上升趋势。研究表明:向日葵幼苗对低浓度(1~10 mg•L-1)镍胁迫能通过自身调节,增加POD、Pro和MDA等物质的含量来提高对生态环境的抗逆能力,说明向日葵幼苗对重金属镍具有一定抗性,而高浓度(50~100 mg•L-1)镍胁迫会破坏其自身防御系统,影响幼苗的生长发育。

  • 金槐和米槐主流品种花粉形态研究

    Subjects: Biology >> Botany >> Applied botany submitted time 2018-10-26 Cooperative journals: 《广西植物》

    Abstract:本研究以 6 个金槐品种和 2 个米槐品种的花粉为材料,采用美国卡尔蔡司 EVO18 扫描电镜对其极面、赤道面、外壁纹饰等形态特征进行了观察,并对金槐、米槐品种间的亲缘关系进行了分析。结果表明:金槐和米槐花粉的共有特征包括:极面观为三裂圆形,极面光滑;具 3 孔沟,沟较窄且达两极;沟缘中部外凸,具颗粒状突起;沟在极面上没有交会,形成宽大的沟界极区;金槐的花粉粒为长球形,金槐 J6 的极轴最长,金槐 J2 的极轴最短,极轴长/赤道轴长表现的规律与极轴长相一致;网眼直径最大的是金槐 J2,最小的是金槐 J5;网眼密度最大的是金槐 J6,最小的是金槐 J2;米槐的花粉粒为近圆球形,极轴长和极轴长/赤道轴长明显小于金槐,但其赤道轴长大于金槐;双季米槐的赤道轴长、极轴长、极轴长/赤道轴长、网眼密度都小于单季米槐;8 个品种可分为 3 组,其中金槐 J4、金槐 J1 和金槐 J6 3 个品种亲缘关系较近,金槐J5、金槐 J3 和金槐 J2 3 个品种亲缘关系较近,单季米槐和双季米槐 2 个品种亲缘关系较近;花粉形态是鉴别金槐和米槐两大类品种的重要根据,并可为进一步的品种划分提供重要依据。