拟南芥bHLH Ib 转录因子调控FIT 的转录

Subjects: Biology >> Botany >> Applied botany submitted time 2022-06-07 Cooperative journals: 《广西植物》

杨钰洁 梁岗

Abstract： FIT (FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR) is a key regulator of Fe homeostasis, which is up-regulated under Fe deficiency conditions, however,the underlying mechanism is still unclear. In this study, the transcript levels of FIT was determined in the single and dual overexpression plants for bHLH38 and FIT, as well as the bHLH Ib quadruple mutant. Under Fe sufficient conditions, FIT increased in the bHLH38 overexpression plants compared with in the wild type plants. The transcription of FIT did not respond to Fe deficiency in the bHLH Ib quadruple mutant. The overexpression of FIT could not activate the transcription of native FIT, and the dual overexpression of FIT and bHLH38 promoted the transcription of native FIT under Fe sufficient conditions. There was no difference for the expression of FIT between the transgenic or mutant plants and wild type plants under Fe deficiency conditions. Taken together, these data suggest that bHLH Ib transcription factors are necessary, but not sufficient, for the upregulation of FIT by Fe deficiency.

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