Subjects: Biology >> Botany >> Applied botany submitted time 2022-05-21 Cooperative journals: 《广西植物》
Abstract： The theory and practice of artificial cultivation are based on the environmental regulation of the secondary metabolism of medicinal plants. However, so far, the research on the accumulation effect of short-term ultraviolet radiation on the medicinally active ingredients was still relatively weak. In this study, the light environment-sensitive plant Dictamnus dasycarpus was taken as the research object, and the inductive effect of short-term enhanced ultraviolet radiation (UV) on four active ingredients, obacunone, fraxinellone, dictamnine, and limonin in the roots, stems, and leaves of D. dasycarpus was explored. The results were as follows: (1) Under whether low- or medium-intensity UV-A and UV-B radiation conditions, the maximum quantum yield of photosystem II (Fv/Fm) of D. dasycarpus was always over 0.76 during the experimental period. Compared with the control (without ultraviolet radiation enhancement treatment), there was no significant differences in the actual photosynthetic quantum yield of photosystem II Y (II), regulated energy dissipation quantum yield Y(NPQ), photochemical quenching coefficient (qL) and the non-photochemical quenching coefficient (NPQ). Low- and medium-intensity UV-B radiation significantly promoted the increase of non-regulated energy dissipation quantum yield Y (NO) of D. dasycarpus. (2) An appropriate enhanced amount of short-term ultraviolet radiation could promote the accumulation of the active ingredients of D. dasycarpus, the four active ingredients in the roots could be increased by up to 51%, the accumulation is mainly in the D. dasycarpus roots. The effects of moderate radiation intensity UV-A radiation and lower intensity UV-B radiation were the most obvious, and it not only promoted the accumulation of four active ingredients of obacunone, fraxinellone, dictamnine, and limonin in the roots, but also promoted the accumulation of dictamnine in the stem and fraxinellone in the leaves. The results reveal that the effectiveness of the accumulation of active ingredients in D. dasycarpus is improved under short-term enhanced ultraviolet radiation conditions. It also reveals that an effective way to improve the tolerance of the D. dasycarpus to light intensity and promote the accumulation of active ingredients is to increase the non-photochemical efficiency of the D. dasycarpus photosynthetic system.
Hits
Hits
Downloads
Comment