Subjects: Biology >> Botany >> Applied botany submitted time 2022-07-05 Cooperative journals: 《广西植物》
Abstract： The purpose of this study is to investigate the main antioxidant active components in Olea europaea pomace. The antioxidant ability of different extracts from O. europaea pomace was determined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH·) free radical scavenging method. Furthermore, the antioxidant compounds were extracted with solvents, isolated by various column chromatography (silica gel, ODS and Sephadex LH-20), and identified by nuclear magnetic resonance spectrum (NMR). The results were as follows: The ethyl acetate extracts of O. europaea exhibited potent antioxidant activity, characterized with half-inhibitory concentration (IC50) at 119.11 μg·mL-1; Seventeen compounds, gallic acid (1), hydroxytyrosol (2), protocatechuic acid (3), tyrosol (4), catechin (5), vanillic acid (6), caffeic acid (7), vanillin (8), syringic acid (9), cynaroside (10), oleuropein (11), eugenol (12), quercetin (13), luteolin (14), rutin (15), maslinic acid (16), oleanolic acid (17), were isolated from the antioxidant part of O. europaea pomace. Among them, compounds 1, 6, 7, 9, and 15 were isolated from O. europaea pomace for the first time. The study identified the material basis of O. europaea pomace against oxidative stress, which provides a reference for its development and high-value application.
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