Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》
Abstract： The paper clarifies the molecular bioinformatics characteristics and tissue expression patterns of the COBRA gene family members of Huperzia serrata, to provide a basis for further research on COBRA genes. Based on the full-length transcriptome data of the Huperzia serrata, the physicochemical properties, domains, conserved motifs, cis-acting elements, and genes expression of the family members ( HsCOBRAs ) were analyzed by bioinformatics techniques. The results were as follows: (1) A total of 24 HsCOBRAs family members were screened in the full-length transcriptome of Huperzia serrata, including 9 acidic proteins, 11 stabilizing proteins, 5 hydrophobic proteins, 7 proteins with transmembrane structures, and 3 proteins with signal peptides. (2) Subcellular localization was found in the cell wall, chloroplast, nucleus, and cell membrane. (3) Structural analysis revealed that HsCOBRAs have 7 domains and 6 conserved motifs, and partial members have a highly conserved CCVS structure. (4) HsCOBRAs have 46 cis-acting elements such as CAAT-box and TATA-box. (5) HsCOBRA2 had the highest expressionin in leaves, spores, stems and gemma. The molecular bioinformatics and expression characterization of the COBRA gene family of Huperzia serrata can provide theoretical basis for further research and biological function verification of HsCOBRAs.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-12-24 Cooperative journals: 《广西植物》
Abstract： To explore the effects of nitrogen, phosphorus and potassium on the physiology of Pyrrosia petiolosa as well as the synthesis and accumulation of chlorogenic acid, tissue culture seedlings of P. petiolosa were used as materials, and three concentration gradients of low nutrient ( no fertilization : N0, P0, K0 ), normal fertilization ( N : 0.2 g·kg-1, P : 0.15g·kg-1, K : 0.15 g·kg-1 ) and high nutrient ( N1 : 0.4 g·kg-1, P1 : 0.3 g·kg-1, K1 : 0.3 g·kg-1) were set up. There were seven treatments set up: NPK、N0PK、N1PK、NP0K、NP1K、NPK0 and NPK1, and the resistance physiological indexes, chlorogenic acid content and key enzyme activities of P. petiolosa under different treatments were determined. The results were as follows: (1) Nitrogen, phosphorus and potassium fertilizers had a significant effect on the resistance physiology of P. petiolosa. The activity of superoxide dismutase(SOD) increased significantly under high nitrogen and low potassium treatments, but catalase (CAT) activity rose significantly under low and high treatments of the three nutrients. (2) The content of chlorogenic acid in P. petiolosa was significantly affected by different levels of nitrogen, phosphorus, and potassium. The chlorogenic acid level in normal fertilization was the highest, reaching 12.92 mg·g-1, while the chlorogenic acid content in high potassium fertilization was the lowest, 7.79 mg·g-1. Potassium fertilizer had the most significant effect on chlorogenic acid content. The content of chlorogenic acid was positively correlated with the activities of quinate o-hydroxycinnamoyl transferase (HQT) and coumaroyl coenzyme ligase (4CL), and negatively correlated with the activity of shikimate o-hydroxycinnamoyl transferase (HCT). HQT, 4CL and HCT were the key factors leading to the difference of chlorogenic acid content. The results of this study can provide a theoretical basis for the artificial cultivation of P. petiolosa.
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