Subjects: Biology >> Botany >> Applied botany submitted time 2019-08-26 Cooperative journals: 《广西植物》
Abstract： To study the effect of cycloartenol synthase gene (CAS) in the sterol biosynthesis of Paeonia delavayi, using the RT-PCR technology, a complete open reading frame of PdCAS gene was cloned from Paeonia delavayi for the first time. The full-length cDNA of PdCAS gene was 2 274 bp, encodes 757 amino acids. The PdCAS protein sequence of P. delavayi had more than 86% similarty with the Prunus persica, Prunus yedoensis var. nudiflora and Vitis vinifera. Sequence alignment analysis showed that PdCAS had a typical conserved DCTAE motif of oxidosaualene cyclase and the marker sequence DGSWYGSWGVCFTYG of triterpenoid synthase．The phylogenetic analysis revealed that PdCAS and Malus domestica (XP 008391430.1), Pyrus bretschneideri (XP 009370034.1), Rosa chinensis(XP 024193310.1), Prunus yedoensis var. Nudiflora (PQQ11009.1), Prunus persica (XP 007225240.1)clustered together. Transcription pattern analysis showed that PdCAS expressed significantly in different tissues and had the highest transcript profile in flowers. PdCAS was closely related to the synthesis of sterols from Paeonia delavayi.
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