Subjects: Biology >> Botany >> Applied botany submitted time 2024-03-26 Cooperative journals: 《广西植物》
Abstract： Rosa praelucens is a critically endangered alpine wild flower endemic to Shangrila County of Yunnan Province. Rich in phenotypic diversity and with a high ploidy level of decaploid, R. praelucens is a very important rose germplasm resource. In order to clarify the genetic background of its phenotypic variation, the chloroplast genomes of 40 individual plants representing different phenotypes within the species were sequenced by using the Illumina HiSeq 2000 platform, and then assembled, annotated and compared. The results were as follows: (1) Chloroplast genomes of R. praelucens were 157 173-157 261 bp in length, with a size difference of 88 bp among different individual plants. The genomes encoded 132 genes, mainly related with photosynthesis and self-replication. 27 155 codons, preferring using codon ending of A or U, were found in all the coding sequences. (2) Thirty six repeats and 73 simple sequence repeats (SSRs) were detected in the chloroplast genome of R. praelucens. Most of the cpSSRs were mononucleotide type and located in the intergenic region of LSC region. (3) The haplotype diversity (Hd) among the 40 chloroplast genomes was 0.928±0.027, and the nucleotide diversity (Pi）was 0.00012. The intergenic region of petN-trnD and psaA-ycf3, gene rps16 and ycf1 were relatively more divergent. No reverse or loss of large DNA fragments and genes were found among the cp genomes of different individuals. These results suggested that the chloroplast genomes were highly conserved in size, sequence and structure within R. praelucens. The rich intraspecific phenotypic diversity was not directly caused by the variation of chloroplast genomes among different individual plants.
Hits
Hits
Downloads
Comment