Subjects: Biology >> Botany >> Applied botany submitted time 2023-08-04 Cooperative journals: 《广西植物》
Abstract： With the rapid development of biodiversity informatics, more and more open biological data can be used by researchers. Taking an open data platform as an example, analyzing the research hotspots and development trends in the field of biodiversity in China will help biodiversity researchers and policy-makers keep abreast of the current status and trends of biological research, and provide decision support for the construction of biodiversity in China. We searched the full text of the literature in CNKI and Google Scholar from 2013 to 2023 with the relevant search terms of “National Specimen Information Infrastructure (NSII)”, and retrieved a total of 1 070 NSII-supported literature, including journal articles (822), dissertations (233), popular science articles (5), conference articles (6) and reports (4).Through the means and methods of bibliometrics, the 822 journal articles supported by NSII are explored from the aspects of publication status, research topics and hot spots, research institutions, etc., to explore the current status, hot spots and trends of biodiversity research supported by NSII. According to the results obtained from the keyword co-occurrence network map, the research hotspots of biodiversity focus on the analysis and modeling of species distribution, climate change, taxonomy, biodiversity research, and platform construction. Biodiversity informatics is developing rapidly in China, in the future, it is still necessary to improve data source construction, resource integration, sharing capability, professional competence, and international cooperation to promote the development of biodiversity scientific research continuously.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-06-05 Cooperative journals: 《广西植物》
Abstract： Water content is a key factor influencing the seed cryopreservation, but its effecting mechanism is not fully understood. In order to provide theoretical basis for further improving the seeds viability after cryopreservation, the effect and way of water content on the viability of seed cryopreservation were explored. Malus � robusta seeds with different water contents were obtained by silica gel. The seed viability, sugar content and related enzyme indexes before and after cryopreservation were measured and correlation analysis were done. The results were as follows: (1) After 15 days of cryopreservation, the viability of seeds with different water contents was different, and the viability of seeds showed a trend of increasing and then decreasing as the water content of seeds decreased, and the seeds with 9.02% water content had the highest germination rate, at 53.33%. But after 120 days of cryopreservation, the viability of seeds increased as the water content decreased, and the seeds with 6.40%water content got the highest germination rate, at 27.78%. This indicates that the water content of Malus � robusta seeds has a significant effect on the viability after cryopreservation. It is affected by the storage time in liquid nitrogen, and the optimum water content decreases as the storage time extended in liquid nitrogen. (2) Correlation analysis showed that water content was significantly negatively correlated with viability, both water content and viability were correlated with sucrose and fructose content, acid invertase was correlated with water content, viability, sucrose and fructose, and fructokinase was correlated with viability, suggesting that water content affects the content of sucrose and fructose through effecting the activity of acid converting enzymes, which in turn affects sucrose metabolism to respond to low temperature and dehydration stress, ultimately leading to differences in viability. The seed viability is also affected by fructose metabolism through fructose kinase. Besides, alginate is one of the important regulatory substances of Malus � robusta seeds in response to water and low temperature stresses. In summary, water content has a significant effect on the viability of Malus � robusta seeds cryopreservation through the combined effect of sucrose and fructose metabolism. This study provides a theoretical reference to further explore the cryopreservation mechanism of seed.
Hits
Hits
Downloads
Comment