Subjects: Biology >> Botany >> Applied botany submitted time 2021-12-19 Cooperative journals: 《广西植物》
Abstract： Calcineurin B-like calcium sensor (CBL) is a Ca2+-binding protein that mediates the Ca2+ signal transduction process by interacting with Calcineurin B-like calcium sensor interacting protein kinase (CIPK). CBL-CIPK signaling system is involved in the response of plants to a variety of stress conditions. For insight into the chilling mechanism of CBL and CIPK in Jatropha curcas, the CBL and CIPK gene families were identified from J.curcas based on the BLAST method, and then the phylogenetic relationship, gene structure, expression profile, and functional interaction were analyzed. The results were as follows: (1) A total of 8 CBL and 18 CIPK genes were identified from J.curcas genome. The protein length of CBLs and CIPKs ranged from 211 to 257 aa and 422 to 484 aa, respectively, and the putative isoelectric point ranged from 4.65 to 5.08 and 6.20 to 9.26, respectively. (2) Furthermore, the CBL family genes contained 8-10 exons, while the CIPK family genes were divided into 1-2 exons (11 genes) and 12-15 exons (7 genes). (3) Sequence alignment revealed that CBL proteins identified 1 atypical EF-hand motif consisting of 14 amino acid residues and 3 typical EF-hand motifs with different substitution, while CIPK proteins contained kinase domains in N-terminal and self-inhibiting FISL/NAF domains in C-terminal. (4) Chromosome mapping analysis indicated that 26 J.curcas CBL and CIPK genes were distributed with different densities on 9 chromosomes. (5) Transcriptome data analysis showed that most of the CBL and CIPK genes were highly expressed in J.curcas leaves, roots, and seeds. Among them, the up-regulated expression of JcCIPK14 and JcCIPK18 reached significant levels under cold stress, which was involved in the cold resistance of J.curcas. The results of this study might lay a significant foundation for further studies on the gene function and chilling signaling transduction mechanism of CBL and CIPK gene families in J.curcas.
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