Subjects: Biology >> Botany >> Applied botany submitted time 2023-07-26 Cooperative journals: 《广西植物》
Abstract： Based on the analysis of introduction and survival records, this paper reports the status of introduction, survival, and ex-situ conservation of plant species in the South China National Botanical Garden (SCNBG). The results show that: (1) A total of 19 154 species, 99 subspecies and 136 varieties (80 597 accession number) of vascular plants belonging to 325 families and 3 952 genera have been introduced since 1956, of which 11 581 species, 52 subspecies and 80 varieties (24 352 accession number) belonging to 290 families and 2 777 genera are surviving currently; the introduction records include 565 species of National Key Protected Wild Plants (421 species survived), covering 95% (36/38) of the first-class National Key Protected Wild Plants (29 species survived), 76.4%（269/352）of the second-class National Key Protected Wild Plants (269 species survived), and 54.3%（547/1 008）threatened species (414 species survived) in South China; (2) The most species are introduced from Guangdong Province (7 193 accession numbers, 2 523 species), followed by Hainan Province (3 658 accession numbers, 1 593 species), Guangxi Province (4 744 accession numbers, 1 559 species) and other surrounding provinces; the SCNBG has obtained some precious plant resources from the same latitude regions by introducing and exchanging species with 61 countries around the world; (3) 14 specialized gardens for ex-situ conservation based on plant taxa (e.g. Magnolia Garden) show a high level in terms of introduction quantity and survival rate; (4) The correlation between the number of surviving species and the frequency of introduction is extremely significant (r = 0.85***); (5) The survival rate of introduced species distributed naturally in South China is higher than that in other provinces. In the future, the SCNBG needs to strengthen the ex-situ conservation of plants through following points: (1) On the basis of investigation, cataloguing, evaluation and research, strengthen the introduction and collection of rare and endangered plants, native plants, and economically important plants in tropical and subtropical regions, further improving the quantity and quality of ex-situ conservation; (2) Establish an ex-situ conservation network system for plants in South China to effectively protect plant diversity in this region; (3) Improve the infrastructure and information management system of ex-situ conservation for higher efficiency; (4) Enhance international scientific research cooperation and species exchange.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-12-19 Cooperative journals: 《广西植物》
Abstract： C2H2 zinc finger proteins are a class of important transcription factors in eukaryotes, which play important roles in plant growth and development and in response to abiotic stresses. OsZAT12, a C2H2 zinc finger protein in rice, which cloned in our previous study, was only expressing in rice roots and localized in the nucleus. Overexpressing OsZAT12 in Arabidopsis exhibited dwarf phenotype. To further investigate the function of OsZAT12 in rice, qRT-PCR was used to analyze the response patterns of OsZAT12 under abiotic stresses and phytohormones treatment, and the promoter elements and transcriptional activity of OsZAT12 was analyzed as well. The results show that OsZAT12 contains two typical C2H2 zinc finger domains and one EAR motif, and has transcriptional repressive activity. The promoter of the OsZAT12 contains elements related to abiotic stresses and phytohormones. The results of abiotic stresses and phytohormones treatment in rice also revealed that low temperature stress (4癈) and phytohormone ABA treatment significantly down-regulated OsZAT12 expression, while osmotic stress (20% PEG 6000), phytohormone BR or IAA treatment significantly up-regulated the expression of OsZAT12. These results indicate that OsZAT12 involves in the changes in response to abiotic stresses and phytohormones in rice. Homozygous OsZAT12 overexpression plants and OsZAT12 knockout plants were obtained using overexpression vector with 35S promoter and CRISPR/Cas9 gene editing technology, respectively. Observation of the phenotype of OsZAT12 overexpression rice showed that compared with the wild type, the plant height of OsZAT12 overexpression plants was significantly shorter at tillering stage, heading stage and maturity stage. The plant height of OsZAT12 knockout plants did not change significantly comparing with the wild type, while the panicle number and seed setting rate of them were significantly lower than those of the wild type. These results indicated that OsZAT12 affected the establishment of agronomic traits such as rice plant type, panicle type and seed setting rate. The results in this study further showed that overexpression of OsZAT12 reduces the sensitivity of rice to exogenous ABA, while the opposite phenotype was observed in OsZAT12 knockout plants. Taken together, it is speculated that the effect of OsZAT12 on plant growth and development might be related to the regulation of this gene in response to abiotic stresses and hormonal signals. This study might provide an experimental basis for molecular design breeding of stress-tolerant and stable yield using OsZAT12 in rice.
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